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Plasmid#84010Purposeexpresses a TEAD-binding mutant of YAP (YAPS94A) in mammalian cells under the control of a doxycycline-inducible promoterDepositorInsertYAP1 (siRNA insensitive) (YAP1 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationS94APromoterTRE-CMVAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFP.E222
Plasmid#138248PurposeThree input / three output logic circuit plasmid - Expresses different intein split ECFs from different inducible promotersDepositorInsertsaraC (araC )
ECF20.N.(VDA).M86.N2
Terminator
SspGyrB.C2.(SAK).ECF16.C
Terminator
rhaS
ECF17.N.(NPC).NrdJ-1.N2
Terminator
M86.C2.(SDL).ECF20.C
Terminator
luxR
Terminator
ECF16.N.(AGG).SspGyrB.N2
Terminator
NrdJ-1.C2.(SEI).ECF17.C
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23115, P(araBAD), P(lux2), P(rhaBAD), iJ23101, a…Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CAG-NOTCH2NL-delta EGF repeats (N2NL-delta EGF)-ires-EGFP
Plasmid#122955PurposeLentiviral expression of NOTCH2NL delta EGF repeats under CAG promoter with EGFP expressionDepositorInsertNOTCH2NL-delta EGF repeats (NOTCH2NLB Human)
UseLentiviralTags6xhis tagExpressionMutationdeletion of EGF repeats from full length NOTCH2NLBPromoterCAG promoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAB(EXPR-DS-HDAC5_HDAC3)
Plasmid#114399PurposeFor PYL-HDAC5 catalytic domain swap to HDAC3 catalytic domain expressionDepositorUseTags3xFlag-NLS (internal)ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-3nitroY-A7-RS
Plasmid#141173PurposeExpression of orthogonal tRNA and tRNA synthetase for incorporation of unnatural amino acidsDepositorInsertA7 3nY tRNA synthetase
UseLentiviralTagsExpressionMammalianMutationP186T L274R N311G C313NPromoterCMVAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-haloTyrRS C6
Plasmid#141174PurposeExpression of orthogonal tRNA and tRNA synthetase for incorporation of unnatural amino acidsDepositorInsertC6 HaloTyr tRNA synthetase
UseLentiviralTagsExpressionMammalianMutationL270S, Y271L, N311G, C313TPromoterCMVAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV CCTG 1200x
Plasmid#63088PurposeAdenoAssociated Virus plasmid containing expanded repeats--CCTG 1200x that correspond to Myotonic Dystrophy of type 2DepositorInsertCCUG 1200x
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCI_FLAG-GFP(Y39TAG)-6His
Plasmid#223507PurposeFluorescent reporter plasmid for genetic code expansion which consists of a modified GFP with a Amber stop codon on position Y39DepositorInsertGFP(Y39TAG)
UseTags6xHis and FLAGExpressionMammalianMutationY39TAGPromoterAvailable sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV CTG 700x
Plasmid#63087PurposeAdenoAssociated Virus plasmid containing expanded repeats--CTG 700x that is the mutation found in Myotonic Dystrophic type 1DepositorInsertCTG REPEATS
UseAAVTagsEGFPExpressionMammalianMutationCTG 700x that is the mutation found in Myotonic D…PromoterCMVAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pMAH2-CageCys
Plasmid#71404PurposeExpresses tRNA and synthetase for mammalian encoding of a photocaged cysteineDepositorInsertaminoacyl-tRNA synthetase for a caged cysteine
UseTagsExpressionMutationPromoterCMVAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAS_MmaPylT_EF1_NES-MmaPylRS WT
Plasmid#174901Purposeexpression of Mma PylRS with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integrationDepositorInsertMma PylRS (pylS Methanosarcina mazei)
UseTagsFLAG and NESExpressionMammalianMutationPromoterEF1Available sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-DsRed FP (pMIDsRed II)
Plasmid#52110Purposea bicistronic IRES-DsRed containing retroviral vector, MCS is expanded from pMSCV-IRES-GFPDepositorTypeEmpty backboneUseRetroviralTagsIRES-DsRedExpressionMammalianMutationPromoterAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-YFP II (pMIY II)
Plasmid#52108Purposea bicistronic IRES-YFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFPDepositorTypeEmpty backboneUseRetroviralTagsIRES-EYFPExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-CFP II (pMIC II)
Plasmid#52109Purposea bicistronic IRES-CFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFPDepositorTypeEmpty backboneUseRetroviralTagsIRES-ECFPExpressionMammalianMutationPromoterAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAS_MmaPylT_EF1_NES-MmaPylRS AF
Plasmid#174902Purposeexpression of Mma PylRS AF with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integrationDepositorInsertMma PylRS (pylS Methanosarcina mazei)
UseTagsFLAG and NESExpressionMammalianMutationY306A, Y384FPromoterEF1Available sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP II (pMIG II)
Plasmid#52107Purposea bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFPDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagsIRES-EGFPExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pULTRA-CNF
Plasmid#48215Purposehighly efficient suppressor plasmidDepositorInsertspolyspecific MJ tyrosyl synthetase
tRNA
UseTagsExpressionBacterialMutationY32L, L65V, F108W, Q109M, D158G, I159APromoterproK and tacIAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
iD20 LoxStopLox-Zim3-dCas9-mApple-hygro
Plasmid#204720PurposeCre-inducible CRISPRi by Zim3-dCas9InsertZim3-dCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3E-CMVmin:GFPmem pA
Plasmid#80823PurposeCMVmin-promoted GFPmem with pADepositorInsert3E-CMV-GFPCAAXpA spacer
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only