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Plasmid#144632PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertETV5 (ETV5 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_Granulin 2+linker3
Plasmid#176920Purposeexpress Granulin 2 with linker 3 in mammalian cellsDepositorInsertGranulin 2+linker3 (GRN Human)
UseTagsTwin-Strep and FLAG tags encoded after Signal Pep…ExpressionMammalianMutationPromoterAvailable sinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
FLINC-AKAR1-CAAX
Plasmid#87705PurposeFluorescent biosensor for super-resolution imaging of PKA activity; targeted to plasma membrane.DepositorInsertFLINC-AKAR1-CAAX
UseTagsC-terminal targeting sequence from KrasExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_Paragranulin-no linker
Plasmid#176917Purposeexpress Paragranulin without linker 1 in mammalian cellsDepositorInsertParagranulin (GRN Human)
UseTagsTwin-Strep and FLAG tags encoded after Signal Pep…ExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+_FH-AGO2-5xA
Plasmid#92007PurposeExpresses FLAG-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]DepositorInsertAGO2 (AGO2 Human)
UseTagsFLAG-HAExpressionMammalianMutationS824A, S828A, T830A, S831A, S834A (5xA)PromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF0636
Plasmid#142282PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertHOXC5 (HOXC5 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+_FH-AGO2-5xE
Plasmid#92008PurposeExpresses FLAG-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]DepositorInsertAGO2 (AGO2 Human)
UseTagsFLAG-HAExpressionMammalianMutationS824E, S828E, T830E, S831E, S834E (5xE)PromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_Granulin 2-no linker
Plasmid#176921Purposeexpress Granulin 2 without linker 3 in mammalian cellsDepositorInsertGranulin 2 (GRN Human)
UseTagsTwin-Strep and FLAG tags encoded after Signal Pep…ExpressionMammalianMutationPromoterAvailable sinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_Granulin 1+linker2
Plasmid#176918Purposeexpress Granulin 1 with linker 2 in mammalian cellsDepositorInsertGranulin 1+linker2 (GRN Human)
UseTagsTwin-Strep and FLAG tags encoded after Signal Pep…ExpressionMammalianMutationPromoterAvailable sinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNAI-GAL4-CREB-S133A
Plasmid#46770PurposeExpression of GAL4 fusion to CREB-S133ADepositorUseTagsGAL4ExpressionMammalianMutationChanged CREB serine 133 to AlaninePromoterCMVAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNAI-GAL4-CREB-S142A
Plasmid#46771PurposeExpression of GAL4 fusion to CREB-S142ADepositorUseTagsGAL4ExpressionMammalianMutationChanged CREB serine 142 to AlaninePromoterCMVAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
Lyn-FLINC-EKAR1
Plasmid#87707PurposeFluorescent biosensor for super-resolution imaging of ERK activity; targeted to plasma membrane.DepositorInsertLyn-FLINC-EKAR1
UseTagsN-terminal targeting sequence from Lyn kinaseExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Exp-pcDNA3.2delCMV(EF1α-tTA/TetO-mCh-Rs1)
Plasmid#26797DepositorInsertEF1a-tTA/TetO-mCherry-P2A-Rs1 (HTR4 Human)
UseTagsExpressionMammalianMutationHuman 5HT4B receptor with D100A mutation, generat…PromoterAvailable sinceFeb. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
FN3K gRNA (BRDN0001148311)
Plasmid#77432Purpose3rd generation lentiviral gRNA plasmid targeting human FN3KDepositorInsertFN3K (FN3K Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
FN3K gRNA (BRDN0001148047)
Plasmid#77433Purpose3rd generation lentiviral gRNA plasmid targeting human FN3KDepositorInsertFN3K (FN3K Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
FN3K gRNA (BRDN0001145506)
Plasmid#77431Purpose3rd generation lentiviral gRNA plasmid targeting human FN3KDepositorInsertFN3K (FN3K Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_Granulin 4+linker5
Plasmid#176924Purposeexpress Granulin 4 with linker 5 in mammalian cellsDepositorInsertGranulin 4+linker5 (GRN Human)
UseTagsTwin-Strep and FLAG tags encoded after Signal Pep…ExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lyn-FLINC-EKAR1(TA)
Plasmid#87708PurposeNegative-control mutant of Lyn-FLINC-EKAR1.DepositorInsertLyn-FLINC-EKAR1(TA)
UseTagsN-terminal targeting sequence from Lyn kinaseExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pLCHKO_intergenic-intergenic_1
Plasmid#155077PurposeLentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases (CHyMErA system), respectivelyDepositorInsert(hg)RNA targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only