Addgene

We narrowed to 11,983 results for: lic

Showing: 1841 - 1860 of 11983 results

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pSec7-Halox2

Plasmid

#115425

Purpose

Express Sec7-Halox2 at the endogenous locus via pop-in pop-out mutagenesis

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

Sec7-Halox2

Use

Tags

HaloTag

Expression

Yeast

Mutation

Promoter

Endogenous

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-vps10-104

Plasmid

#115426

Purpose

Generate the vps10-104 allele via pop-in pop-out mutagenesis

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

vps10-104

Use

Tags

Expression

Yeast

Mutation

domain 1 deletion

Promoter

Endogenous

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pI12

Plasmid

#24273

Depositor

Mariano Garcia-Blanco

Article

Baraniak et al Mol Cell Biol. 2003 Dec . 23(24):9327-37

Insert

modified adenovirus exons and introns

Use

T7 for in vitro transcription

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 30, 2010

Availability

Academic Institutions and Nonprofits only

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YIplac128-pOst1-ESCargo

Plasmid

#115421

Purpose

Expresses a regulatable secretory cargo for yeast at the LEU2 locus

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

pOst1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

Use

Tags

Expression

Yeast

Mutation

Promoter

TPI1

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-D-TOPO-kcnn1a

Plasmid

#164956

Purpose

Zebrafish kcnn1a gene CDS

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

Insert

kcnn1a (kcnn1a Zebrafish)

Use

Pcr cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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pRS406-GFP-SNC1(L96V)

Plasmid

#105278

Purpose

Integrating construct for expressing GFP-Snc1(L96V) from the TPI1 promoter.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Snc1 (SNC1 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

Leucine 96 changed to Valine, intron removed

Promoter

TPI1

Available since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pUASt-ssBiP-ESCargo

Plasmid

#140166

Purpose

Expresses a regulatable secretory cargo for Drosophila melanogaster

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

ssBip-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

Use

Tags

Expression

Insect

Mutation

Promoter

hs70

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pSec7-iGFPx6

Plasmid

#115424

Purpose

Express Sec7-iGFPx6 at the endogenous locus via pop-in pop-out mutagenesis

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

Sec7-iGFPx6

Use

Tags

iGFP

Expression

Yeast

Mutation

Promoter

Endogenous

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pHYRSF236

Plasmid

#46377

Depositor

Hideo Iwai

Article

Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

Insert

NpuDnaE intein delta variant, inactive

Use

Tags

H6-Smt3

Expression

Bacterial

Mutation

C1A, C+1A, deleted T76, V97, D98, L100 from NpuDn…

Promoter

T7

Available since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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psc2_md1

Plasmid

#61093

Purpose

splicing reporter construct containing MAG mini-gene

Depositor

Sean Ryder

Article

Zearfoss et al RNA. 2013 Jul;19(7):948-57. doi: 10.1261

Insert

Mag genomic sequence (Mag Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

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YIplac211-RPL13A-FKBPx4

Plasmid

#104474

Purpose

Plasmid for pop-in/pop-out replacement of RPL13A with an FKBPx4-tagged version.

Depositor

Benjamin Glick

Article

Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi:

Insert

RPL13Ap (RPL13A Budding Yeast)

Use

Tags

FKBPx4-HA

Expression

Yeast

Mutation

includes the last 354 bp of RPL13A only

Promoter

Available since

Feb. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pJBM2

Plasmid

#225289

Purpose

E. coli-Campylobacter shuttle vector; KanR

Depositor

Robert Moore

Article

McDonald et al mBio. 2024 Jul 29:e0110124. doi: 10.1128

Insert

Replication elements from pCC311 for pJBM2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pJBM3

Plasmid

#225290

Purpose

E. coli-Campylobacter shuttle vector; KanR

Depositor

Robert Moore

Article

McDonald et al mBio. 2024 Jul 29:e0110124. doi: 10.1128

Insert

Replication elements from pCC388 for pJBM3

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1_W208L

Plasmid

#221486

Purpose

NKX2-1 W208L DNA binding mutation lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

W208L

Promoter

EF1a

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1

Plasmid

#221484

Purpose

NKX2-1 short isoform lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-UBC_NKX2-1

Plasmid

#221495

Purpose

NKX2-1 short isoform lentiviral overexpression using UBC promoter

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

UBC

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_Long

Plasmid

#221471

Purpose

Gateway cloning of NKX2-1 long isoform overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_W208L

Plasmid

#221474

Purpose

Gateway cloning of NKX2-1 W208L DNA binding mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

W208L

Promoter

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_V205F

Plasmid

#221473

Purpose

Gateway cloning of NKX2-1 V205F DNA binding mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

V205F

Promoter

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1_Long

Plasmid

#221483

Purpose

NKX2-1 long isoform lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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