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Plasmid#115425PurposeExpress Sec7-Halox2 at the endogenous locus via pop-in pop-out mutagenesisDepositorInsertSec7-Halox2
UseTagsHaloTagExpressionYeastMutationPromoterEndogenousAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-vps10-104
Plasmid#115426PurposeGenerate the vps10-104 allele via pop-in pop-out mutagenesisDepositorInsertvps10-104
UseTagsExpressionYeastMutationdomain 1 deletionPromoterEndogenousAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pI12
Plasmid#24273DepositorInsertmodified adenovirus exons and introns
UseT7 for in vitro transcriptionTagsExpressionMammalianMutationPromoterAvailable sinceMarch 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
YIplac128-pOst1-ESCargo
Plasmid#115421PurposeExpresses a regulatable secretory cargo for yeast at the LEU2 locusDepositorInsertpOst1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnn1a
Plasmid#164956PurposeZebrafish kcnn1a gene CDSDepositorInsertkcnn1a (kcnn1a Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterAvailable sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS406-GFP-SNC1(L96V)
Plasmid#105278PurposeIntegrating construct for expressing GFP-Snc1(L96V) from the TPI1 promoter.DepositorInsertSnc1 (SNC1 Budding Yeast)
UseTagsGFPExpressionYeastMutationLeucine 96 changed to Valine, intron removedPromoterTPI1Available sinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUASt-ssBiP-ESCargo
Plasmid#140166PurposeExpresses a regulatable secretory cargo for Drosophila melanogasterDepositorInsertssBip-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionInsectMutationPromoterhs70Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSec7-iGFPx6
Plasmid#115424PurposeExpress Sec7-iGFPx6 at the endogenous locus via pop-in pop-out mutagenesisDepositorInsertSec7-iGFPx6
UseTagsiGFPExpressionYeastMutationPromoterEndogenousAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHYRSF236
Plasmid#46377DepositorInsertNpuDnaE intein delta variant, inactive
UseTagsH6-Smt3ExpressionBacterialMutationC1A, C+1A, deleted T76, V97, D98, L100 from NpuDn…PromoterT7Available sinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
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YIplac211-RPL13A-FKBPx4
Plasmid#104474PurposePlasmid for pop-in/pop-out replacement of RPL13A with an FKBPx4-tagged version.DepositorInsertRPL13Ap (RPL13A Budding Yeast)
UseTagsFKBPx4-HAExpressionYeastMutationincludes the last 354 bp of RPL13A onlyPromoterAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJBM2
Plasmid#225289PurposeE. coli-Campylobacter shuttle vector; KanRDepositorInsertReplication elements from pCC311 for pJBM2
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJBM3
Plasmid#225290PurposeE. coli-Campylobacter shuttle vector; KanRDepositorInsertReplication elements from pCC388 for pJBM3
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-EF1a_NKX2-1_W208L
Plasmid#221486PurposeNKX2-1 W208L DNA binding mutation lentiviral overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationW208LPromoterEF1aAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-EF1a_NKX2-1
Plasmid#221484PurposeNKX2-1 short isoform lentiviral overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-UBC_NKX2-1
Plasmid#221495PurposeNKX2-1 short isoform lentiviral overexpression using UBC promoterDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterUBCAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_NKX2-1_Long
Plasmid#221471PurposeGateway cloning of NKX2-1 long isoform overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_NKX2-1_W208L
Plasmid#221474PurposeGateway cloning of NKX2-1 W208L DNA binding mutation overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseGateway donor vectorTagsExpressionMutationW208LPromoterAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_NKX2-1_V205F
Plasmid#221473PurposeGateway cloning of NKX2-1 V205F DNA binding mutation overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseGateway donor vectorTagsExpressionMutationV205FPromoterAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-EF1a_NKX2-1_Long
Plasmid#221483PurposeNKX2-1 long isoform lentiviral overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only