Addgene

We narrowed to 2,510 results for: fas

Showing: 1841 - 1860 of 2510 results

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LifeAct-mGreenLantern

Plasmid

#164459

Purpose

Localization of mGreenLantern fluorescent protein to actin

Depositor

Gregory Petsko

Article

Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

mGreenLantern

Use

Tags

LifeAct

Expression

Mammalian

Mutation

Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

Promoter

CMV

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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p416-TEF-membrane localization signal-tagBFP-wrmScarlet11-TEF terminator

Plasmid

#158585

Purpose

Expresses membrane localized tagBFP with wrmScarlet11 C-terminal fusion in S. cerevisiae

Depositor

Cynthia Kenyon

Article

Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

Insert

membrane localization signal-tagBFP-wrmScarlet11

Use

Tags

tagBFP

Expression

Yeast

Mutation

GGGS linker

Promoter

TEF

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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ER-mGreenLantern

Plasmid

#164463

Purpose

Localization and retention of mGreenLantern fluorescent protein in the ER

Depositor

Gregory Petsko

Article

Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

mGreenLantern

Use

Tags

Calreticulin and KDEL

Expression

Mammalian

Mutation

Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

Promoter

CMV

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLPCX2-HA-GATA3 FL

Plasmid

#89835

Purpose

Express GATA3 in mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

Insert

Gata3 (Gata3 Mouse)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

CMV

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pTol2 hsp70l loxP-DsRed-loxP-nCas9n-GFP

Plasmid

#158962

Purpose

Cre-dependet Cas9-GFP expression

Depositor

Michael Brand

Article

Hans et al Nat Commun. 2021 Feb 18;12(1):1125. doi:

Inserts

hsp70l promoter
floxed DsRed cassette
Cas9-GFP

Use

CRISPR and Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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H2B-mGreenLantern

Plasmid

#164464

Purpose

Localization of mGreenLantern fluorescent protein to histone 2B

Depositor

Gregory Petsko

Article

Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

mGreenLantern

Use

Tags

Human Histone 2B (D26G and V119I mutations)

Expression

Mammalian

Mutation

Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

Promoter

CMV

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pET-45b-POLR2A-CTD-mCherry

Plasmid

#185011

Purpose

Bacterial Expression of 6xHis C-terminal domain of POLR2A mCherry Fusion Protein

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

POLR2A (Polr2a Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-dLight1.3b

Plasmid

#135762

Purpose

For expression of the genetically encoded dopamine sensor dLight1.3b

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Has Service

AAV9

Insert

dLight1.3b

Use

AAV

Tags

Flag tag

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-Smad4

Plasmid

#89829

Purpose

Knockdown human Smad4 expression in human mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2011 Nov 11;286(45):38865-7

Insert

Smad4 shRNA (SMAD4 Mouse, Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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NLS-Laconic/pCMV-myc-nuc

Plasmid

#46307

Purpose

a nuclear-targeted, genetically-encoded sensor for lactate

Depositor

Luis Felipe Barros

Article

Barros et al Front Cell Neurosci. 2013;7:27. doi: 10.

Insert

NLS-Laconic

Use

Lactate biosensor

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

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Mito-mGreenLantern

Plasmid

#164461

Purpose

Localization of mGreenLantern fluorescent protein to mitochondria

Depositor

Gregory Petsko

Article

Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

mGreenLantern

Use

Tags

Human COX8a first 29 amino acids

Expression

Mammalian

Mutation

Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

Promoter

CMV

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-dLight1.2

Plasmid

#111068

Purpose

To generate Adeno-Associated viruses for expression of dLight1.2 under synapsin promoter

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Has Service

AAV1 and AAV5

Insert

dLight1.2

Use

AAV

Tags

Flag tag

Expression

Mutation

Promoter

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-CMV_TetR-HA-NLS-P2A-BSD-Myc

Plasmid

#113899

Purpose

Mammalian constitutive expression of HA-tagged Tetracyclin repressor protein and Myc-tagged blasticidin deaminase

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Insert

TetR-HA-NLS-P2A-BSD-Myc

Use

Lentiviral

Tags

HA-Myc

Expression

Mutation

Promoter

CMV-MIE

Available since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-dLight1.1

Plasmid

#111067

Purpose

To generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoter

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Has Service

AAV1 and AAV5

Insert

dLight1.1

Use

AAV

Tags

Flag tag

Expression

Mutation

Promoter

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only
pAAV-CAG-dLight1.3b

Plasmid

#125560

Purpose

For expression of the genetically encoded dopamine sensor dLight1.3b

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Has Service

AAV1, AAV5, and AAV9

Insert

dLight1.3b

Use

AAV

Tags

Flag tag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-dLight1.1

Plasmid

#111066

Purpose

To generate Adeno-Associated viruses for expression of dLight1.1 under synapsin promoter

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Has Service

AAV1

Insert

dLight1.1

Use

AAV

Tags

Flag tag

Expression

Mutation

Promoter

Available since

July 23, 2019

Availability

Academic Institutions and Nonprofits only
pcDNA3.1/Puro-CAG-ASAP2s

Plasmid

#101274

Purpose

Genetically encoded voltage indicator ASAP2s expressed under strong mammalian promoter (CAG)

Depositor

Francois St-Pierre

Article

Chamberland et al Elife. 2017 Jul 27;6. pii: e25690. doi:

Insert

ASAP2s

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pCSN068

Plasmid

#101749

Purpose

Single copy yeast plasmid expressing Cpf1 from Francisella novicida U112 (FnCpf1), codon optimized for expression in Saccharomyces cerevisiae.

Depositor

Rene Verwaal

Article

Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

Inserts

Cpf1 from Francisella novicida U112 (FnCpf1) codon optimized for expression in S. cerevisiae.
KanMX marker expression cassette.

Use

CRISPR

Tags

SV40 NLS

Expression

Yeast

Mutation

Promoter

Heterologous TEF1 promoter from A. gossypii. and …

Available since

Jan. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2+8 Flag2AUF1(p45)

Plasmid

#135947

Purpose

Expresses AUF1p45 in mammalian cells. Can also be used to generate mRNA for zebrafish expression.

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)

Use

Tags

Flag2

Expression

Mammalian

Mutation

Promoter

CMV and SP6

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2+8 Flag3HuR

Plasmid

#135950

Purpose

Expresses HuR in mammalian cells. Can also be used to generate mRNA for zebrafish expression.

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

ELAV like RNA binding protein 1 (ELAVL1 Human)

Use

Tags

Flag3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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