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Plasmid#179837PurposeDoxycycline-inducible expression of eGFPDepositorInserteGFP
UseCRISPR, Synthetic Biology, and TALEN ; Donor plas…TagsExpressionMammalianMutationPromoterTRE3GSAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_His2-GFP11
Plasmid#153511PurposeHis2-GFP11 C-terminal Tag Donor PlasmidDepositorInsertHis2-GFP11
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOC3 smFP-HA-Cacna1e KI
Plasmid#183436PurposeCreON FlpOFF knock-in for smFP-HA-CaV2.3 (amino acid position: G5)DepositorInsertgRNA and smFP HA donor
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_Kma-Inulinase SP
Plasmid#153492PurposeKluyveromyces marxianus Inulinase Signal Peptide Donor PlasmidDepositorInsertInulinase SP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-22:CETN2-mTagRFP-T
Plasmid#101781PurposeHomology arms and mTagRFPT-linker sequence for N-terminus tagging of human CETN2DepositorInsertCETN2 Homology Arms with mTagRFPT-linker (CETN2 Human)
UseCRISPR; Donor templateTagsmTagRFPT-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceOct. 31, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTF-ahpC-rfp
Plasmid#153035PurposeFor CRISPR-Cas12a genome editing in E. coli. Contains a constituitively expressed CRISPR array targeting the ahpC gene in E. coli and donor DNA to insert an rfp translational fusion in the ahpC gene.DepositorInsertCRISPR array
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-83:EZH2-mEGFP
Plasmid#164499PurposeHomology arms and mEGFP-linker sequence for N-terminus tagging of human EZH2DepositorInsertEZH2 Homology Arms with mEGFP-linker (EZH2 Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceFeb. 17, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCR1075
Plasmid#111093PurposeExpresses eGFP donor in pUC19DepositorInserteGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOC1 Gria1-HA KI
Plasmid#183427PurposeCreON knock-in for GluA1-HA (amino acid position: stop codon)DepositorInsertgRNA and HA donor
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOC2 Dlg4-Halo KI
Plasmid#183449PurposeCreOFF knock-in for PSD95-Halo (amino acid position: R721)DepositorInsertgRNA and Halo donor
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-eGFP-pA
Plasmid#107281Purposedonor vector backbone with constitutive eGFPDepositorInsertGFP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOC2 CRISPIE-Halo-Homer1 KI
Plasmid#183446PurposeCreON knock-in for Halo-Homer1 (amino acid position: G2)DepositorInsertgRNA and CRISPIE Halo donor
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJ204-loxP-100RPU.2-coEPO-lox2272
Plasmid#154828PurposeDonor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing one copy of erythropoietinDepositorInsertcoEPO
UseCre/LoxTagsExpressionMammalianMutationCodon-optimized (Cricetulus griseus)Promoter100RPU.2Available sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
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mMaroon-RTK-mMaroon
Plasmid#84359PurposeFRET acceptor for RhoA donor, flanked with the far-red FP mMaroonDepositorInsertmMaroon-Rtk--mMaroon
UseTagsmMaroon flanking Rtk domain on N and C terminusExpressionMammalianMutationPromoterCMVAvailable sinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_ONECUT2
Plasmid#135738PurposeDonor vector for 3' FLAG tag of human ONECUT2DepositorInsertONECUT2 Homology arms (ONECUT2 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceJan. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
LT2_14_GlyOx
Plasmid#177711PurposePlasmid for genome integration in X2 site expressing GlyRNA and OxPro (biosensors for glycolytic flux and oxidative stress)DepositorInsertspTEFmut8-mTurquoise2-RNA_2.6--tCYC1
pTEFmut8-mCherry-tADH1
pTRX2_5xUAS-ymYPET-tCYC1
UseUsed as donor dna in genome integration after lin…TagsExpressionYeastMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOC4 Actb-mRuby3 KI
Plasmid#183437PurposeFlpON knock-in for mRuby3-beta-actin (amino acid position: before start codon)DepositorInsertgRNA and mRuby3 donor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOC5 Tubb3-GFP KI
Plasmid#183440PurposeFlpOFF knock-in for beta3-tubulin GFP (amino acid position: stop codon)DepositorInsertgRNA and GFP donor
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_TwinStrep-GFP11
Plasmid#153514PurposeTwinStrep-GFP11 C-terminal Tag Donor PlasmidDepositorInsertTwinStrep-GFP11
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only