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Plasmid#79194PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlac62 gIII, luxAB; Ptet rpoZ-p38_2_3
UseTagsExpressionBacterialMutationPromoterPlac62; PtetAvailable sinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pGEX4T-1-mMcl-1(S140A)
Plasmid#25381DepositorInsertmMCL1(S140A) (Mcl1 Mouse)
UseTagsGstExpressionBacterialMutationchanged serine 140 to alaninePromoterAvailable sinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAB043a
Plasmid#79222PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ lambda cI-MBP; PlacIq lacI
UseTagsExpressionBacterialMutationPromoterPlacZ; PlacIqAvailable sinceApril 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAB082f
Plasmid#79238PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPpro1 rpoZ-Cry1Ac-d23A
UseTagsExpressionBacterialMutationPromoterPpro1Available sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
VCDstub-GFP
Plasmid#33122DepositorInsertPcdh-γA3 (Pcdhga3 Mouse)
UseTagsEGFPExpressionMammalianMutationContains a signal sequence fused to a very small …PromoterCMVAvailable sinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pIL2R FAK Y397F
Plasmid#50525DepositorInsertprotein tyrosine kinase, mutated
UseTagsIL-2RExpressionMammalianMutationY397FPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
p201N 1514
Plasmid#47025PurposeContains soybean miRNA miR1514 recognition sequence to produce siRNAs from 3' target sequences and induce RNA silencingDepositorInsertsgma-miR1514 recognition sequence
nptII selectable marker
UseRNAiTagsExpressionMutationPromoterGmUbi and StUbi-3Available sinceAug. 30, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
Wnt8A STOP
Plasmid#35879DepositorInsertWnt8A (Wnt8a Human)
UseEntry vectorTagsExpressionMutationSilent Mutation A (600) --> GPromoterAvailable sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
ΔCT190-GFP
Plasmid#33118DepositorInsertPcdh-γA3 (Pcdhga3 Mouse)
UseTagsEGFPExpressionMammalianMutationDeletion of the last 190 aa of the variable cytop…PromoterCMVAvailable sinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pEN_TmiR_G13-L
Plasmid#25762PurposeEntry vector with TRE promoter driving mouse G alpha 13 miR30-based shRNA.DepositorInsertG alpha 13 miR-shRNA
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1-mMcl-1(T144A)
Plasmid#25382DepositorInsertmMCL1(T144A) (Mcl1 Mouse)
UseTagsGstExpressionBacterialMutationchanged threonine 144 to alaninePromoterAvailable sinceOct. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR v2-sgControl
Plasmid#230080PurposeCrispr knock out controlDepositorInsertnon-specific sequence control
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pWN_U6-TRAC-miniGag
Plasmid#228960PurposeminiEDV production plasmid. Expresses the miniGag polypeptide (CAG promoter) and sgRNA (human U6 promoter)DepositorInsertminiGag
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-fDIO-stGtACR2-FusionRed
Plasmid#217975PurposeAAV transfer plasmid to express stGtACR2-FusionRed in a frt-dependent mannerDepositorInsertstGtACR2-FusionRed
UseAAVTagsFusionRedExpressionMutationPromoterEF1aAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDF-Leup
Plasmid#213154PurposeExpresses the leupeptin protease inhibitor pathway for bolstered production of heterologously expressed proteins.DepositorInsertLeupeptin Protease Inhibitor Pathway
UseTagsExpressionBacterialMutationPromoterT7Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPath-pTac-integrase
Plasmid#199999PurposeEntry vector for biosynthetic pathway. pTac-phiC31 integrase. This material contains 2 plasmids. For more information look under the "Depositor Comments" section below.DepositorInsertPathways
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
Dox-SOScat
Plasmid#207329PurposePiggyBac vector backbone encoding doxycycline-inducible expression of membrane-localized catalytic domain of SOS (SOScat)DepositorInsertson of sevenless homolog 2 variant, catalytic domain
UseTagsmyristoylation tag - BFPExpressionBacterialMutationrange: 593 to 1078PromoterTet Response ElementAvailable sinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only