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Plasmid#124122PurposeBacterial expression plasmid for PURE system componentsDepositorInsertValyl-tRNA ligase (valS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a-LeuRS-His
Plasmid#124113PurposeBacterial expression plasmid for PURE system componentsDepositorInsertLeucyl-tRNA ligase (leuS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a-HisRS-His
Plasmid#124111PurposeBacterial expression plasmid for PURE system componentsDepositorInsertHistidyl-tRNA ligase (hisS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a-GluRS-His
Plasmid#124109PurposeBacterial expression plasmid for PURE system componentsDepositorInsertGlutamyl-tRNA ligase (gltX Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a-CysRS-His
Plasmid#124107PurposeBacterial expression plasmid for PURE system componentsDepositorInsertCysteinyl-tRNA ligase (cysS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ii-Str_neomycin
Plasmid#65308PurposeLuminal ER hook only (RUSH system)DepositorInsertInvariant chain (p33)
UseTagsExpressionMammalianMutationStreptavidin in its lumenal partPromoterCMVAvailable sinceAug. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
VSVGwt-SBP-EGFP_puromycin
Plasmid#65302PurposeVSVG reporter only (no hook) (RUSH system)DepositorInsertVSV-G
UseTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
Str-Ii_VSVG-SBP-EGFP
Plasmid#65300Purposesynchronize trafficking of VSVG from the ER (RUSH system)DepositorInsertVSV-G
UseTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST44-AP-1 core
Plasmid#184809PurposeBacterial expression of clathrin adaptor AP-1 core complexDepositorInsertHs AP1S3; Hs AP1B1 (1-584); Mm AP1G1 (1-595); Mm AP1M1
UseTagsGST tag and His6ExpressionBacterialMutationAP1S3: S148C; AP1B1: K359R, E476KPromoterT7Available sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_neomycin
Plasmid#65306PurposeLuminal ER hook only (RUSH system)DepositorInsertStreptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end
UseTagsKDEL motif and Signal SequenceExpressionMammalianMutationPromoterCMVAvailable sinceJune 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsTAB182_K
Plasmid#146777PurposeMammalian Expression of HsTAB182DepositorInsertHsTAB182 (TNKS1BP1 Human)
UseTagsExpressionMammalianMutation2 silent mutations compared to the sequence given…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJRA108
Plasmid#209341PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mCherry fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJRA107
Plasmid#209340PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mNeonGreen fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
C1QBP
Plasmid#32863PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertC1QBP (C1QBP Human)
UseTagsHisExpressionBacterialMutationamino acids E89-Q282 onlyPromoterAvailable sinceJan. 6, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLEX_307_ms2_VPR
Plasmid#79376PurposeRecruits VPR to compatible gRNAs for transcriptional activationDepositorInsertms2-VPR
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLEX_307_ms2_p65_hsf1
Plasmid#79374PurposeRecruits p65_hsf1 to compatible gRNAs for transcriptional activationDepositorInsertms2-p65-hsf1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDH_Str-Ii
Plasmid#65313PurposeCytoplasmic ER hook only (RUSH system)DepositorInsertInvariant chain (p33)
UseLentiviralTagsExpressionMutationStreptavidin in its cytoplasmic partPromoterCMVAvailable sinceAug. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJRA110
Plasmid#209343PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal StayGold fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Ii-Str_SBP-EGFP-Ecadherin
Plasmid#65288Purposesynchronize trafficking of Ecadherin from the ER (RUSH system)DepositorInsertEcadherin
UseTagsEGFP and IL-2 Signal Sequence and Streptavidin Bi…ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONRPEX18Km-SceI-pheS
Plasmid#111610PurposeGateway compatible allelic exchange vector for Burkholderia sp.DepositorTypeEmpty backboneUseAllelic exchange in burkholderia sp.TagsExpressionMutationPromoterAvailable sinceAug. 7, 2018AvailabilityAcademic Institutions and Nonprofits only