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Plasmid#206283PurposeBacterial expression of Cas9-His Δcys E532C variant under T7 promoterDepositorInsertSpCas9
UseCRISPRTags6x-His and SV40 NLSExpressionBacterialMutationC80S, E532C, C574SPromoterT7Available sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
CSTI.pJL1
Plasmid#199113PurposeIn vitro expression of glycosyltransferase from Campylobacter jejuni for elaboration of sialic acid onto galactose in α2,3 linkage using CMP-sialic acid as substrateDepositorInsertCSTI
UseTagsCAT linker, Strep-tagExpressionBacterialMutationPromoterT7Available sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Derp2.pJL1
Plasmid#199116PurposeIn vitro expression of Der p 2, a major common allergen from Dermatophagoides pteronyssinus (dust mite), with synthetic site for glycosylation encoded (GGNWTT)DepositorInsertDer p 2 dust mite allergen
UseTagsGlyctag, His-tagExpressionBacterialMutationPromoterT7Available sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-RLuc-HsAsb9-3UTR_X
Plasmid#147949PurposeMammalian Expression of HsAsb9-3UTRDepositorInsertHsAsb9-3UTR (ASB9 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmHPat_57-684_J
Plasmid#146694PurposeInsect Expression of DmHPat_57-684DepositorInsertDmHPat_57-684 (Patr-1 Fly)
UseTagsExpressionInsectMutationTwo silent mutations compared to the sequence giv…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmHPat-del57-499_H
Plasmid#146460PurposeInsect Expression of DmHPat-del57-499DepositorInsertDmHPat-del57-499 (Patr-1 Fly)
UseTagsExpressionInsectMutationone silent mutation C2340A compared to the sequen…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-HA-DmHPat_1-499_H
Plasmid#146461PurposeInsect Expression of DmHPat_1-499DepositorInsertDmHPat_1-499 (Patr-1 Fly)
UseTagsExpressionInsectMutationtwo silent mutations compared to the sequence giv…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-HA-DmHPat_1-684_H
Plasmid#146462PurposeInsect Expression of DmHPat_1-684DepositorInsertDmHPat_1-684 (Patr-1 Fly)
UseTagsExpressionInsectMutationTwo silent mutations compared to the sequence giv…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-HA-DmHPat_57-499_H
Plasmid#146463PurposeInsect Expression of DmHPat_57-499DepositorInsertDmHPat_57-499 (Patr-1 Fly)
UseTagsExpressionInsectMutationtwo silent mutations compared to the sequence giv…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-HA-DmHPat-del57-499-dsRNAres_H
Plasmid#146465PurposeInsect Expression of DmHPat-del57-499-dsRNAresDepositorInsertDmHPat-del57-499-dsRNAres (Patr-1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-HA-DmHPat-dsRNAres_H
Plasmid#146467PurposeInsect Expression of DmHPat-dsRNAresDepositorInsertDmHPat-dsRNAres (Patr-1 Fly)
UseTagsExpressionInsectMutationtwo silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmHPat_1-684_H
Plasmid#146468PurposeInsect Expression of DmHPat_1-684DepositorInsertDmHPat_1-684 (Patr-1 Fly)
UseTagsExpressionInsectMutationTwo silent mutations compared to the sequence giv…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmHPat_57-968_H
Plasmid#146469PurposeInsect Expression of DmHPat_57-968DepositorInsertDmHPat_57-968 (Patr-1 Fly)
UseTagsExpressionInsectMutationtwo silent mutations compared to the sequence giv…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmHPat-del500-684_H
Plasmid#146473PurposeInsect Expression of DmHPat-del500-684DepositorInsertDmHPat-del500-684 (Patr-1 Fly)
UseTagsExpressionInsectMutationthree silent mutations compared to the sequence g…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX601‐mhCMV‐miniABEmax‐N3‐ E53ogRNA
Plasmid#187064PurposeNpu Split N-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutationDepositorInsertminiABEmax-Npu
UseAAV and CRISPRTagsExpressionMammalianMutationPromotermhCMVAvailable sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmHPat_57-499_B
Plasmid#145979PurposeInsect Expression of DmHPat_57-499DepositorInsertDmHPat_57-499 (Patr-1 Fly)
UseTagsExpressionInsectMutationtwo silent mutations compared to the sequence giv…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmMe31B_1-267_D
Plasmid#146118PurposeInsect Expression of DmMe31B_1-267DepositorInsertDmMe31B_1-267 (me31B Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmHPat_1-225_A
Plasmid#145870PurposeInsect Expression of DmHPat_1-225DepositorInsertDmHPat_1-225 (Patr-1 Fly)
UseTagsExpressionInsectMutationone non silent mutation N218K compared to the seq…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmHPat_500-968_A
Plasmid#145873PurposeInsect Expression of DmHPat_500-968DepositorInsertDmHPat_500-968 (Patr-1 Fly)
UseTagsExpressionInsectMutationone silent mutation A1758G compared to the sequen…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmHPat_1-225_A
Plasmid#145875PurposeInsect Expression of DmHPat_1-225DepositorInsertDmHPat_1-225 (Patr-1 Fly)
UseTagsExpressionInsectMutationone non silent mutation N218K compared to the seq…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only