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Plasmid#104158PurposeBacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (1x Cysteine)DepositorInsertAnti-mouse IgG1 Fc specific nanobody TP1107 (1xCysteine)
UseTags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDawn
Plasmid#43796PurposeEmpty backbone for blue-light-regulated expression of a gene of interest. Expression is induced under blue illumination.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pN1-CMV-Aff6_V18FdN-IntC-mCherryC-T2A-EGFP (C2)
Plasmid#210725PurposeNIR optogenetic module for conditional protein splicing of split mCherry (C-part)DepositorInsertC2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-H2B_Halo_T2A_EGFP
Plasmid#135444PurposeMamalian cell expression of nuclear HaloTag7 and cytosolic EGFPDepositorInsertH2B-HaloTag7 and EGFP
UseTagsH2BExpressionMammalianMutationPromoterCMV/TetOAvailable sinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-PCNA-IRES-puro2b
Plasmid#26461DepositorInsertPCNA (PCNA Human)
UseTagspEGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
Ki67-mNeonGreen-IRESpuro2
Plasmid#183737PurposeExpresses Ki67-mNeonGreenDepositorInsertMKI67 (MKI67 Human)
UseTagsmNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET16better_H10_HRV3C_chitinase
Plasmid#175091PurposeExpresses B. licheniformis chitinase with an N terminal 10X His tag and HRV-3C cleavage site in E. coli.DepositorInsertchitinase
UseTags10X His, HRV-3CExpressionBacterialMutationPromoterAvailable sinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
H2B-mNeonGreen-IRESpuro2
Plasmid#183745PurposeExpresses H2B-mNeonGreenDepositorInsertH2B (H2BC16P Human)
UseTagsmNeonGreenExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-Sec31-yoHalo
Plasmid#115423PurposeExpress Sec31-yoHalo at the endogenous locus via pop-in pop-out mutagenesisDepositorInsertSec31-yoHalo
UseTagsyoHaloTagExpressionYeastMutationPromoterEndogenousAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmRFP_a_tubulin_C1
Plasmid#21040DepositorInsertalpha Tubulin (TUBA1B Human)
UseTagspmRFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCAG-HA-Nrxn1beta AS4(-)
Plasmid#59409PurposeExpression plasmid for HA-tagged mouse Neurexin 1beta AS4(-) [lacking the insert at alternatively spliced segment 4 (AS4-)]DepositorInsertNrxn1beta AS4(-) (Nrxn1 Mouse)
UseTagsHAExpressionMammalianMutationPromoterCAGAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLSLGFP.R
Plasmid#51501PurposeSingle component LSL vector with GFP. Rep is expressed from the complementary sense LIR promoter. GFP is between LIR and SIR and is driven from a 2X35S promoter. Rep is within the repliconDepositorInsertGFP
UsePlant t-dna plasmidTagsExpressionMutationPromoter2x35SAvailable sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDsEndoNA2
Plasmid#174728PurposeExpresses DsRed-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.DepositorInsertDsRed-Monomer
UseTagsHistagExpressionBacterialMutationPromoterT5Available sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnn2
Plasmid#164958PurposeZebrafish kcnn2 gene CDSDepositorInsertkcnn2 (kcnn2 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCW57-Cx32-IRES-GCaMP6s
Plasmid#216795PurposeInducible bicistronic lentiviral plasmid for the simultaneous expression of Connexin 32 and cytosolic GCaMP6sDepositorInsertConnexin 32-IRES-GCaMP6s (GJB1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
TR therapy reporter
Plasmid#179083PurposeFluorescent reporter for alternative splicingDepositorInsertsDsRed1
EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-TOM20_Halo_T2A_EGFP
Plasmid#135443PurposeMamalian cell expression of mictochondrial HaloTag7 and cytosolic EGFPDepositorInsertTOM20-HaloTag7 and EGFP
UseTagsTOM20ExpressionMammalianMutationPromoterCMV/TetOAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRegev_V1
Plasmid#204143PurposeSingle reporter randomly selected from library for biochemical validationDepositorInsertV1 exon in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTruncated CAGAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-MNN9-msGFP2
Plasmid#177105PurposeExpresses Mnn9-msGFP2 in yeast cellsDepositorInsertMNN9 (MNN9 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCin4-3xFLAG-MKL1
Plasmid#19838DepositorInsertMKL1 (MRTFA Human)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only