Addgene

We narrowed to 2,325 results for: gem

Showing: 1801 - 1820 of 2325 results

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pLITMUS-rpoN-cIopt-J23106-geneIII

Plasmid

#80852

Purpose

Phagemid (encodes M13 gene III and lambda cIopt).

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
Lambda cIopt

Use

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23106 and rpoN

Available since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pRU45

Plasmid

#167667

Purpose

ENTRY vector for gRNA5 cloning under pAtU6 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU6

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pISH-Tshz1

Plasmid

#74360

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Tshz1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

teashirt zinc finger family member 1 (Tshz1 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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YIplac211-ANP1-msGFP2

Plasmid

#177100

Purpose

Expresses Anp1-msGFP2 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

ANP1 (ANP1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pRU46

Plasmid

#167668

Purpose

ENTRY vector for gRNA6 cloning under pAtU3 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU3

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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p1xNLS-FRB-mCherry-hsp86-BSD

Plasmid

#85795

Purpose

Expression of 1xNLS mislocaliser in P. falciparum

Depositor

Tobias Spielmann

Article

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

1xNLS Mislocaliser

Use

P. falciparum expression

Tags

FRB*, mCherry

Expression

Mutation

Promoter

Pfhsp86

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pLyn-FRB-mCherry-nmd3-BSD

Plasmid

#85796

Purpose

Expression of PPM mislocaliser in P. falciparum

Depositor

Tobias Spielmann

Article

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

PPM Mislocaliser

Use

P. falciparum expression

Tags

FRB*, mCherry

Expression

Mutation

Promoter

Pfnmd3

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pMB1-A-pBAD-dCas9

Plasmid

#190132

Purpose

Level 1 MoClo plasmid containing an arabinose inducible dCas9 for CRISPRi

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Insert

dCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pSLI-TGD(1463000)

Plasmid

#85791

Purpose

P. falciparum integration plasmid to disrupt genes using SLI

Depositor

Tobias Spielmann

Article

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

PF3D7146300 targeting region, GFP-2TA-Neo-R

Use

P. falciparum genomic integration (gene disruptio…

Tags

GFP

Expression

Mutation

Promoter

none

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pISH-Pde4a

Plasmid

#105463

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Omura et al Mol Cell Neurosci. 2015 Mar;65:114-24. d

Insert

Pde4a (Pde4a Mouse)

Use

To ganerate riboprobe

Tags

Expression

Mutation

Promoter

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pBSKΔB

Plasmid

#177268

Purpose

Backbone vector for STREAMING-tag knockin vector

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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DT035A

Plasmid

#159397

Purpose

Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli tac promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.tac-sfGFP

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Mutation

Promoter

tac promoter

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pTH-MYCN

Plasmid

#35416

Depositor

William A. Weiss

Article

Weiss et al EMBO J. 1997 Jun 2;16(11):2985-95.

Insert

v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) (MYCN Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Tryosine Hydroxlyase (TH)

Available since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only

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pBluescriptKS(-)-FRB

Plasmid

#104475

Purpose

Plasmid carrying FRB with C-terminal HA tag.

Depositor

Benjamin Glick

Article

Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi:

Insert

FRB (MTOR Human)

Use

Phagemid

Tags

HA tag

Expression

Mutation

includes only the FRB domain

Promoter

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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KFS2MT

Plasmid

#44624

Depositor

Robert Oshima

Article

Cecena et al Am J Pathol. 2006 Jan;168(1):310-20.

Insert

keratin 18 gene (KRT18 Human)

Use

Mouse Targeting

Tags

polyomavirus middle T antigen (PyMT)

Expression

Mutation

Promoter

Available since

June 19, 2013

Availability

Academic Institutions and Nonprofits only

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pLITMUS-rpoN-cI5C6A,P-J23106-geneIII

Plasmid

#80860

Purpose

Phagemid (encodes evolved cI5C6A,P and M13 gene III)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
cI5C6A,P

Use

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23106 and rpoN

Available since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pRU48

Plasmid

#167670

Purpose

ENTRY vector for gRNA8 cloning under pAtU3 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU3

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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K18iresCreER-T2

Plasmid

#44580

Depositor

Robert Oshima

Article

Wen et al Genesis. 2003 Feb;35(2):100-6.

Insert

keratin 18 gene (KRT18 Human)

Use

Mouse Targeting

Tags

IRES_CreER-T2

Expression

Mutation

Promoter

Available since

June 18, 2013

Availability

Academic Institutions and Nonprofits only
DT037A

Plasmid

#159398

Purpose

Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli J23101 (strong) promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.J23101-sfGFP

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Mutation

Promoter

J23101 (strong) promoter

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pBP-TL5

Plasmid

#72989

Purpose

RBS_M2

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

M2 RBS

Use

Synthetic Biology

Tags

Expression

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Promoter

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only