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pCDNA5-LAP-MPS1-dTPR
Plasmid#114048PurposeExpression of exogenous LAP tagged MPS1 delta TPR (TPR is deleted)DepositorInsertMPS1-dTPR (TTK Human)
UseTagsExpressionMammalianMutationdelta TPR (deletion of TPR domain 63-191)PromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5-LAP-MPS1-d200
Plasmid#114047PurposeExpression of exogenous LAP tagged MPS1 delta 200 (NTE and TPR are deleted)DepositorInsertMPS1-d200 (TTK Human)
UseTagsExpressionMammalianMutationdelta 200 (deletion of NTE and TPR domains)PromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5-LAP-MPS1-d60
Plasmid#114046PurposeExpression of exogenous LAP tagged MPS1 delta 60 (NTE is deleted)DepositorInsertMPS1-d60 (TTK Human)
UseTagsExpressionMammalianMutationdelta 60 (deletion of NTE domain)PromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 - human TUBB3 Cterminal tail delta K
Plasmid#60404PurposeExpression of chimeric yeast beta tubulin (TUB2) - human beta3 tubulin Cterminal tail (TUBB3) without last 4 amino acids--QGPKDepositorInsertTUB2
UseTagsExpressionYeastMutationTUB2 amino acids 429 - end, replaced with human T…PromoterGALAvailable sinceMay 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EngPDm
Plasmid#64040PurposeMammalian expression of mutant paired domain of Pax8 fused to the N-terminal repressor domain of the drosophila engrailed protein (Eng)DepositorInsertPax8 paired domain (Pax8 Mouse)
UseTagsEng (N-terminal repressor domain of the drosophi…ExpressionMammalianMutationpaired domain, aa 1-147, with aa 30 to 32 changed…PromoterCMVAvailable sinceApril 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pALOD4
Plasmid#111026PurposeExpresses domain 4 of anthrolysin O cholesterol sensor, has N-terminal His6-tag and two point mutations, S404C and C472ADepositorInsertDomain 4 of Anthrolysin O
UseTagsHis6 tagExpressionBacterialMutationMutations: S404C, C472APromoterT7Available sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-LaminA-C-18
Plasmid#55068PurposeLocalization: Nuclear Envelope, Excitation: 587, Emission: 610DepositorInsertLamin A (LMNA Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
Rc/CMV cyclin D2 HA
Plasmid#8950DepositorInsertcyclin D2 (CCND2 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
mRFP-Rab7
Plasmid#14436PurposeMammalian expression of Rab7 fused to mRFP. Used for localization to the late endosome, and other early endosomal vacuoles.DepositorInsertRab 7 (RAB7A Canis familialis)
UseTagsmRFPExpressionMammalianMutation3 silent mutations near the C-terminusPromoterAvailable sinceMarch 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGL-FLKif3B
Plasmid#13743DepositorInsertmouse Kif3B (Kif3b Mouse)
UseCre/LoxTagsGFPExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
Lamin A (R377H)-mRFP
Plasmid#124270PurposeLamin A mutant (R377H), C-terminally tagged with mRFP. Mutation associated with EDMD myopathy.DepositorInsertLMNA (R377H) (LMNA Human)
UseTagsmRFPExpressionMammalianMutationLMNA (R377H)PromoterAvailable sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pMXs-mRFP1
Plasmid#21315PurposeMoMLV-based Retroviral vector, expresses mRFP1DepositorInsertmonomeric Red Fluorescence Protein 1
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
TRPML3-YFP
Plasmid#18835DepositorInsertTRPML3 (Mcoln3 Mouse)
UseTagsYFPExpressionMammalianMutationQ165R, F210YPromoterAvailable sinceJuly 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLEX_307_ms2_VPR
Plasmid#79376PurposeRecruits VPR to compatible gRNAs for transcriptional activationDepositorInsertms2-VPR
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLEX_307_ms2_p65_hsf1
Plasmid#79374PurposeRecruits p65_hsf1 to compatible gRNAs for transcriptional activationDepositorInsertms2-p65-hsf1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
p-dCas9-HDAC1-Hygro
Plasmid#104409Purposetransient expression of dCas9-HDAC1 fusion proteinDepositorInsertHDAC1 (HDAC1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGFP-E p27 (CC#631)
Plasmid#15192DepositorInsertp27 (Cdkn1b Mouse)
UseRetroviralTagsFlag and HisExpressionMammalianMutationPromoterAvailable sinceJune 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1-GST-eIF4G1-His6
Plasmid#37231DepositorInsertTIF4631 (TIF4631 Budding Yeast)
UseTagsGST and His6ExpressionBacterialMutationPromotertacAvailable sinceSept. 4, 2012AvailabilityAcademic Institutions and Nonprofits only
