Addgene

153,751 results

Showing: 1801 - 1820 of 153751 results

Results per page:

Sort results by:

AAV_CAG_V5-LOV-Turbo-NES

Plasmid

#199670

Purpose

expresses V5-LOV-Turbo-NES in mammalian cells, AAV vector

Depositor

Alice Ting

Article

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo

Use

AAV

Tags

NES and V5

Expression

Mutation

Promoter

CAG

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
459 p3E-2A-nlsEGFPpA

Plasmid

#195969

Purpose

Gateway p3E 2A fluorophore

Depositor

Christian Mosimann, Kristen Kwan

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

2A-nlsEGFP (nuclear)

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDRM18 LTN (Luciferase tdTomato Neo)

Plasmid

#174721

Purpose

Expresses the firefly luciferase gene, E2A, tdTomato, P2A, and the neomycin resistance gene.

Depositor

Wayne Tilley

Article

Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

Inserts

Firefly Luciferase
tdTomato
aph

Use

Lentiviral and Luciferase

Tags

E2A linker and P2A linker

Expression

Mammalian

Mutation

Promoter

MND, MND (off Luc-E2A), and MND (off Luc-E2A-tdTo…

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-UCOE-EF1a-Zim3-dCas9-loxP-P2A-EGFP-loxP

Plasmid

#188773

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9

Use

Lentiviral

Tags

HA-2xNLS and Zim3 KRAB-NLS fusion

Expression

Mutation

Promoter

EF1a

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
JunD

Plasmid

#187904

Purpose

Expresses JunD in transfected cells

Depositor

Jennifer Mitchell

Article

Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

Insert

JunD (Jund Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
RINS1

Plasmid

#107290

Purpose

Plasmid for the expression of the RINS1 sensor

Depositor

Dmytro Yushchenko

Article

Schifferer et al Cell Chem Biol. 2017 Apr 20;24(4):525-53

Insert

RINS1

Use

Tags

sfGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pK27Sumo_His-SUMO-nsp10-nsp14 fusion (SARS-CoV-2)

Plasmid

#169161

Purpose

To express nsp10-nsp14 fusion protein in E. coli

Depositor

John Diffley

Article

Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

Insert

14His-SUMO-nsp10-GGSGGS-nsp14 (ORF1ab SARS-CoV-2, Synthetic)

Use

Tags

14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…

Expression

Bacterial

Mutation

Codon optimised for E. coli

Promoter

T5

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_KEAP1_p.R470S

Plasmid

#82781

Purpose

Gateway Donor vector containing KEAP1, part of the Target Accelerator Plasmid Collection.

Depositor

Matthew Meyerson, Jesse Boehm, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

KEAP1 (KEAP1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

R470S; C624S

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO/Strep-HA-ZAK beta

Plasmid

#141195

Purpose

Expresses Strep-HA-ZAK beta in mammalian cells, can be used to make inducible cell line

Depositor

Simon Bekker-Jensen

Article

Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

Insert

MAP3K20 isoform 2 (MAP3K20 Human)

Use

Tags

Strep, HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-4T1-14-3-3 epsilon GST

Plasmid

#13279

Depositor

Michael Yaffe

Article

Yaffe et al Cell. 1997 Dec 26. 91(7):961-71.

Insert

14-3-3 epsilon (Ywhae Rat)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-TCF4

Plasmid

#109144

Purpose

For the expression of GFP-TCF in mammalian cells

Depositor

Mariann Bienz

Article

Townsley et al Nat Cell Biol. 2004 Jul;6(7):626-33. doi

Insert

TCF7L2 (TCF7L2 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
M51 Super 8x FOPFlash (TOPFlash mutant)

Plasmid

#12457

Purpose

Control for beta-catenin reporter. Contains mutated TCF/LEF binding sites upstream of a luciferase reporter.

Depositor

Randall Moon

Article

Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

Insert

mutant TCF/LEF binding sites (Ctnnb1 )

Use

Luciferase

Tags

Expression

Mammalian

Mutation

mutant TCF/LEF binding sites

Promoter

Available since

March 13, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pSGAb-spe

Plasmid

#122000

Purpose

A sgRNA expression plasmid for genome editing in Acinetobacter baumannii

Depositor

Quanjiang Ji

Article

Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

Insert

none

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Geminin-mStrawberry reporter

Plasmid

#158690

Purpose

This plasmid is a Geminin cell cycle reporter. It has CMV promoter driving the expression of Geminin fused to mStrawberry-pGK-BSD

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mStrawberry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-24a-ELP[V-150]

Plasmid

#67015

Purpose

Expresses ELP[V-150] in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

ELP[V-150]

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pICherryNeo

Plasmid

#52119

Purpose

a bicistronic IRES-mCherry containing vector for mammalian expression (CMV promoter)

Depositor

Dario Vignali

Type

Empty backbone

Use

Tags

IRES-mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE80L-IL2 C125S

Plasmid

#104348

Purpose

Expresses human IL-2 (C125S)

Depositor

Alexander McLellan

Insert

Human IL-2 (C125S) (IL2 Human)

Use

Tags

MRGSHHHHHGS-tag

Expression

Bacterial

Mutation

C125S mutation (TGT -> TCC)

Promoter

T5

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBITE-EGFRvIII-F271-OKT3

Plasmid

#190682

Purpose

Encodes an EGFRvIII-CD3 BITE protein (F271 clone)

Depositor

Scott McComb

Article

Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

Insert

Anti-EGFRvIII-scFv-F271-modularized-OKT3-HisTag

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_KEAP1_p.G333C

Plasmid

#82835

Purpose

Gateway Donor vector containing KEAP1, part of the Target Accelerator Plasmid Collection.

Depositor

Matthew Meyerson, Jesse Boehm, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

KEAP1 (KEAP1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

G333C; C624S

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Fra-2

Plasmid

#187907

Purpose

Expresses Fra-2 in transfected cells

Depositor

Jennifer Mitchell

Article

Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

Insert

Fra2 (Fosl2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge