-
Plasmid#79722PurposeThis plasmid encodes the kinase domain of STK10. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertSTK10 (STK10 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
MK03_HUMAN_D0
Plasmid#79688PurposeThis plasmid encodes the kinase domain of MK03. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertMK03
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
PAK4_HUMAN_D0
Plasmid#79698PurposeThis plasmid encodes the kinase domain of PAK4. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertPAK4 (PAK4 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
PAK6_HUMAN_D0
Plasmid#79703PurposeThis plasmid encodes the kinase domain of PAK6. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertPAK6 (PAK6 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/ TO myc-cCE-bio (cCE-bio)
Plasmid#82473PurposeExpresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal biotin tagged mRNA capping enzyme, inactive formDepositorInsertMyc-NES-mCE (Wt, NLS deletion)-TEV-Bio (Rngtt Synthetic, Mouse)
UseTagsTEV-Biotin and mycExpressionMammalianMutationPromoterCMVAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMal-Halo
Plasmid#209349PurposeProtein expression vector with a TEV cleavable N-terminal 6xHis-MBP and a HRV 3C cleavable C-terminal HaloTag-StepTagIIDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21a-LIC
Plasmid#62303PurposeE. coli expression vector with N-terminal Hexa-HIS and TEV cleavageDepositorTypeEmpty backboneUseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGTvL1-SGC
Plasmid#39188PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneUseTagsGST TEVExpressionBacterialMutationPromoterTacAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMal-mRuby3
Plasmid#209348PurposeProtein expression vector with a TEV cleavable N-terminal 6xHis-MBP and a HRV 3C cleavable C-terminal mRuby3-StepTagIIDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOP5GT
Plasmid#112611PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and GST tag fusions and with C-terminal Avi-tagDepositorTypeEmpty backboneUseTags8His-GST and AviExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLIC-SGC1
Plasmid#39187PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneUseTagsHis TevExpressionBacterialMutationPromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOP5BT
Plasmid#112609PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and GB1 tag fusions and with C-terminal Avi-tagDepositorTypeEmpty backboneUseTags8His-GB1 and AviExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGGsH6-MCS_SHORT_inFrame
Plasmid#211438PurposeVector backbone for random polymerase chain reaction screenDepositorInsertMCS-tev cleavage site-EGFP-6xHis-Tag
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOP5TT
Plasmid#112613PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and thioredoxin tag fusions and with C-terminal Avi-tagDepositorTypeEmpty backboneUseTags8His-thioredoxin and AviExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
Ubiquiton In Vitro Kit
Plasmid Kit#1000000242PurposeThe Ubiquiton system allows the inducible, linkage-specific polyubiquitylation of a protein of interest, accommodating for linear (M1-), K48-, and K63-linkage.DepositorAvailable sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
CLM24 ∆lpxM
Bacterial Strain#132780PurposeCLM24 strain with lpxM deletionDepositorBacterial ResistanceNoneAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK793
Plasmid#8827DepositorInsertTEV protease, S219V mutant
UseTagsHis, MBP (with TEV), and polyarginineExpressionBacterialMutationS219V mutation (improves stability)PromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
Ubp8/PET32a
Plasmid#26965DepositorInsertUbp8 (UBP8 Budding Yeast)
UseTagsHis6 and TrxExpressionBacterialMutationTEV site is added t the 5' end and 4 alanine…PromoterAvailable sinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
L3MBTL3 (3UT1)
Plasmid#36901PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertL3MBTL3 (L3MBTL3 Human)
UseTagsHis tag and Streptavidin-Binding Peptide (SBP)-Ta…ExpressionBacterialMutationcontains amino acid residues 228-551 onlyPromoterAvailable sinceJune 21, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKM263-USP7
Plasmid#196365Purposeprotein expression of the GST-tagged catalytic domainDepositorInsertUSP7 catalytic domain
UseTagsHis6-GST-TEVExpressionBacterialMutationcontains only S207-E560PromoterT7Available sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only