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Plasmid#80761PurposeExpress Nuf2 N-terminally labeled with mTurquoise2DepositorInsertNuf2 (NUF2 Human)
UseRetroviralTagsmTurquoise2ExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-puro-eGFP-HEC1-9A
Plasmid#114023PurposeInducible expression of eGFP-tagged HEC1-9A in FLPin Trex cell linesDepositorInserteGFP-HEC1 - 9A (NDC80) (NDC80 Human)
UseTagsExpressionMammalianMutationHec1 - 9APromoterAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z09
Plasmid#120052PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mVenusDepositorInsertmVenus
UsePcr templateTagsmVenusExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCherry-eDHFR
Plasmid#107266PurposemCherry-eDHFR in pIVT vector for in vitro transcriptionDepositorInsertmCherry-eDHFR (dhfr E.coli)
UseTagsmCherryExpressionMammalianMutationPromoterT7Available sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTubulin-BC2T
Plasmid#108970Purposeexpresses tubulin-BC2-tag in mammalian cellsDepositorInserta-tubulin
UseTagsBC2-tagExpressionMammalianMutationPromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-KG-WASP-GBD
Plasmid#30113DepositorUseTagsGSTExpressionBacterialMutationContains the Cdc42-interacting domain of WASP (aa…PromoterAvailable sinceSept. 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
KG#371
Plasmid#110882PurposeExpresses the lysosome marker CTNS-1-mCherry cDNA in a subset of ventral cord cholinergic motor neuronsDepositorInsertsunc-129 promoter
CTNS-1a-mCherry cDNA
unc-54 3' control region with 1 intron just upstream and 1 intron in control region
UseTagsintron-mCherryExpressionBacterialMutationPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-hygro-eGFP-HEC1
Plasmid#114027PurposeInducible expression of eGFP-tagged HEC1 in FLPin Trex cell linesDepositorInserteGFP-HEC1 - Wildtype (NDC80) (NDC80 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-optoB2-LYY-YFP
Plasmid#75271PurposeG-protein independent version of optoB2 with point mutations at L72F,Y136G,Y224ADepositorInsertG-protein independent version of optoB2
UseTagseYFPExpressionMammalianMutationpoint mutations at L72F,Y136G,Y224APromoterAvailable sinceOct. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P02
Plasmid#120013PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with TagBFPDepositorInsertTagBFP
UsePcr templateTagsTagBFPExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P25
Plasmid#120036PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with YFP-LIDDepositorInsertYFP-LID
UsePcr templateTagsYFP-LIDExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDGE496
Plasmid#153248PurposeM6 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE495
Plasmid#153247PurposeM5 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEX-A-U6-gRNA
Plasmid#65626PurposeEmpty vector for the expression U6 driven gRNADepositorInsertU6-gRNA
UseTagsExpressionMutationPromoterAvailable sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHO_pTEFmut7_CggR_R250A_ble
Plasmid#124585PurposePlasmid for genomic integration of CggR_R250A repressor regulated by the pTEFmut7 promoterDepositorInsertcggR_R250A (cggR Synthetic)
UseSynthetic BiologyTagsExpressionMutationchanged arginine 250 to alaninePromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPDS1-mEGFP-IRES-puro2b
Plasmid#26464DepositorInsertpPDS1 (PTTG1 Human)
UseTagsmEGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z11
Plasmid#120054PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mScarlet-iDepositorInsertmScarlet-i
UsePcr templateTagsmScarlet-iExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z17
Plasmid#120060PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mEos4bDepositorInsertmEos4b
UsePcr templateTagsmEos4bExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRL_yEGFP_hc
Plasmid#98746PurposeReporter plasmid for the PhiReX system. Encodes the reporter gene yEGFP under the control of the CYC1 minimal promoter with upstream synTALE-DBS.DepositorInsertYeast-enhanced green fluorescent protein
UseSynthetic BiologyTagsExpressionYeastMutationPromotersyntheticAvailable sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYZ_3WJrep_N20_switch
Plasmid#132722PurposeT7 RNAP-driven expression of 3WJ repressor index 20 switch RNA with a GFP-ASV reporterDepositorInsert3WJ_N20_switch
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only