Addgene

We narrowed to 3,077 results for: zebrafish

Showing: 161 - 180 of 3077 results

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pKHR7

Plasmid

#74624

Purpose

a cmlc2::mCherry (red heart) reporter gene was inserted at the 3’end of pKHR4 mcs, that resides within donor fragments produced by I-SceI digestion. Genbank accession number is KU144824.

Depositor

David Grunwald

Article

Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:

Insert

cmlc2::mCherry

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

cmlc2

Availability

Academic Institutions and Nonprofits only

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pHD066

Plasmid

#84032

Purpose

Vector for creating transgenic zebrafish. Expresses mCherry-Cre fusion protein under the elastase promoter and Venus under the crystalin promoter in zebrafish

Depositor

Didier Stainier, Daniel Hesselson

Article

Hesselson et al Curr Biol. 2011 Apr 26;21(8):712-7. doi:

Inserts

Cre
Venus

Use

Cre/Lox; Zebrafish transgenesis

Tags

mCherry

Expression

Mutation

Promoter

Crystalin and Elastase

Availability

Academic Institutions and Nonprofits only

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pTol-Promoter:NLS-tdMCP-eGFP

Plasmid

#86248

Purpose

For generating transgenic lines that ubiquitously expressing the fluorescent MCP reporter

Depositor

Florence Marlow

Article

Campbell et al Development. 2015 Apr 1;142(7):1368-74.

Insert

beta Actin promoter:NLS-tdMCPeGFP

Use

Used with transposase to make transient or stable…

Tags

NLS-tdMCP-eGFP

Expression

Mutation

Promoter

Beta Actin

Availability

Academic Institutions and Nonprofits only

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pDEST mfap4:tdTomato

Plasmid

#135214

Purpose

Red tagged macrophages

Depositor

David Tobin

Article

Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

Insert

mfap4 promoter and tdTomato

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pDEST mfap4:turquoise2

Plasmid

#135218

Purpose

Cyan tagged macrophages

Depositor

David Tobin

Article

Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

Insert

mfap4 promoter and mTurquoise2

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pSP64T-ythdf2-3xflag

Plasmid

#164489

Purpose

Expresses zebrafish ythdf2 with 3x flag tag, for mRNA injection

Depositor

Antonio Giraldez

Article

Kontur et al Cell Rep. 2020 Dec 29;33(13):108598. doi

Insert

ythdf2 (ythdf2 Zebrafish)

Use

Production of mrna for zebrafish injection.

Tags

3x-flagtag

Expression

Mutation

Promoter

SP6

Availability

Academic Institutions and Nonprofits only

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pSP64T-ythdf3-3xflag

Plasmid

#164490

Purpose

Expresses zebrafish ythdf3 with 3x flag tag, for mRNA injection

Depositor

Antonio Giraldez

Article

Kontur et al Cell Rep. 2020 Dec 29;33(13):108598. doi

Insert

ythdf3 (ythdf3 Zebrafish)

Use

Production of mrna for zebrafish injection.

Tags

3x-flagtag

Expression

Mutation

Promoter

SP6

Availability

Academic Institutions and Nonprofits only

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pCS2+methylated-reporter

Plasmid

#164491

Purpose

Reporter to test methylated mRNA stability, for mRNA injection

Depositor

Antonio Giraldez

Article

Kontur et al Cell Rep. 2020 Dec 29;33(13):108598. doi

Insert

A-less CDS, HA tag, 12x m6A motif 3'UTR

Use

Production of mrna for zebrafish injection.

Tags

HA tag

Expression

Mutation

Promoter

SP6

Availability

Academic Institutions and Nonprofits only

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pTol2-eab2-AA

Plasmid

#182153

Purpose

Expression of artificial intron separated mApple

Depositor

Hui Zong

Article

Xu et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

AA

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

eab2

Availability

Academic Institutions and Nonprofits only

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pTol2-eab2-GG

Plasmid

#182154

Purpose

Expression of artificial intron separated eGFP

Depositor

Hui Zong

Article

Xu et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

GG

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

eab2

Availability

Academic Institutions and Nonprofits only

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MiniCoopR mitfa:NRAS

Plasmid

#118847

Purpose

Expresses human NRAS Q61R mutant and zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

NRAS Q61R (NRAS Human, Zebrafish)

Use

Zebrafish expression, tol3

Tags

Expression

Mutation

NRAS Q61R

Promoter

Availability

Academic Institutions and Nonprofits only

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acta1-Cavin4b-Clover

Plasmid

#163854

Purpose

Zebrafish muscle specific expression of Cavin4b-Clover. Parton lab clone DQZ

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

Cavin4b (cavin4b Zebrafish)

Use

Zebrafish expression

Tags

Clover

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pT2h70CRET(#325)

Plasmid

#184060

Purpose

cyclofen-inducible CRE activation in zebrafish transient transgenic

Depositor

Sophie Vriz

Article

Feng et al Sci Rep. 2017 Aug 23;7(1):9195. doi: 10.

Insert

CRE-ERT2

Use

Zebrafish transient transgenesis

Tags

Expression

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Zf-Hsp70-4

Availability

Academic Institutions and Nonprofits only

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tol2-mpx-Dendra2

Plasmid

#29574

Depositor

Anna Huttenlocher

Article

Yoo et al J Leukoc Biol. 2011 Jan 19. ():.

Insert

mpx-Dendra 2

Use

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pDONR221-erg

Plasmid

#192728

Purpose

Gateway entry vector encoding zebrafish erg

Depositor

Summer Thyme

Insert

erg (erg Zebrafish)

Use

Gateway entry vector

Tags

Expression

Mutation

G199D

Promoter

None

Availability

Academic Institutions and Nonprofits only

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pDONR221-nrip1a

Plasmid

#192731

Purpose

Gateway entry vector encoding zebrafish nrip1a

Depositor

Summer Thyme

Insert

nrip1a (LOC796407 Zebrafish)

Use

Gateway entry vector

Tags

Expression

Mutation

E662Q; S895P; H967Q

Promoter

None

Availability

Academic Institutions and Nonprofits only

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pDONR221-cxadr

Plasmid

#192734

Purpose

Gateway entry vector encoding zebrafish cxadr

Depositor

Summer Thyme

Insert

cxadr (cxadr Zebrafish)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

None

Availability

Academic Institutions and Nonprofits only

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pDONR221-btg3

Plasmid

#192735

Purpose

Gateway entry vector encoding zebrafish btg3

Depositor

Summer Thyme

Insert

btg3 (btg3 Zebrafish)

Use

Gateway entry vector

Tags

Expression

Mutation

H211Y

Promoter

None

Availability

Academic Institutions and Nonprofits only

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gfap-EGFP donor

Plasmid

#65564

Purpose

Intron targeting-mediated EGFP knockin at the zebrafish gfap locus

Depositor

Jiu-lin Du

Article

Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

Insert

gfap-EGFP (gfap Zebrafish)

Use

Zebrafish injection

Tags

Expression

Mutation

Promoter

none

Availability

Academic Institutions and Nonprofits only

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MiniCoopR mitfa:BRAF

Plasmid

#118846

Purpose

Expresses human BRAF V600E mutant and zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

BRAF V600E (BRAF Human, Zebrafish)

Use

Zebrafish expression, tol2

Tags

Expression

Mutation

BRAF V600E

Promoter

Availability

Academic Institutions and Nonprofits only

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