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Plasmid#74624Purposea cmlc2::mCherry (red heart) reporter gene was inserted at the 3’end of pKHR4 mcs, that resides within donor fragments produced by I-SceI digestion. Genbank accession number is KU144824.DepositorInsertcmlc2::mCherry
UseZebrafish expressionTagsExpressionMutationPromotercmlc2Available sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
PhOTO-N (pMTB:memb-Cerulean-2A-H2B-Dendra2)
Plasmid#92400PurposeConstitutive expression of nuclear green-to-red photoconvertible Dendra2 and the membrane blue fluorescent protein Cerulean.DepositorInsertsDendra2
Cerulean
UseZebrafish expressionTagsH2B tag and membrane tagExpressionMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST mfap4:tdTomato
Plasmid#135214PurposeRed tagged macrophagesDepositorInsertmfap4 promoter and tdTomato
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
PhOTO-M (pMTB:memb-Dendra2-2A-H2B-Cerulean)
Plasmid#92401PurposeConstitutive expression of membrane green-to-red photoconvertible Dendra2 and the nuclear blue fluorescent protein Cerulean.DepositorInsertsDendra2
Cerulean
UseZebrafish expressionTagsH2B tag and membrane tagExpressionMutationPromoterbactin2Available sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHD066
Plasmid#84032PurposeVector for creating transgenic zebrafish. Expresses mCherry-Cre fusion protein under the elastase promoter and Venus under the crystalin promoter in zebrafishDepositorInsertsCre
Venus
UseCre/Lox; Zebrafish transgenesisTagsmCherryExpressionMutationPromoterCrystalin and ElastaseAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT2-eab2-[GTR]
Plasmid#46144DepositorInsertEGFP/mCherry
UseCre/Lox; Tol2 zebrafish transgenic expressionTagsExpressionMutationPromoterAvailable sinceJuly 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTol-Promoter:NLS-tdMCP-eGFP
Plasmid#86248PurposeFor generating transgenic lines that ubiquitously expressing the fluorescent MCP reporterDepositorInsertbeta Actin promoter:NLS-tdMCPeGFP
UseUsed with transposase to make transient or stable…TagsNLS-tdMCP-eGFPExpressionMutationPromoterBeta ActinAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTol2-eab2-GG
Plasmid#182154PurposeExpression of artificial intron separated eGFPDepositorInsertGG
UseZebrafish expressionTagsExpressionMutationPromotereab2Available sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTol2-eab2-AA
Plasmid#182153PurposeExpression of artificial intron separated mAppleDepositorInsertAA
UseZebrafish expressionTagsExpressionMutationPromotereab2Available sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2+methylated-reporter
Plasmid#164491PurposeReporter to test methylated mRNA stability, for mRNA injectionDepositorInsertA-less CDS, HA tag, 12x m6A motif 3'UTR
UseProduction of mrna for zebrafish injection.TagsHA tagExpressionMutationPromoterSP6Available sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSP64T-ythdf2-3xflag
Plasmid#164489PurposeExpresses zebrafish ythdf2 with 3x flag tag, for mRNA injectionDepositorInsertythdf2 (ythdf2 Zebrafish)
UseProduction of mrna for zebrafish injection.Tags3x-flagtagExpressionMutationPromoterSP6Available sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2-OXER1-mKate2
Plasmid#226713Purposeoverexpression red fluorescent OXER1 protein in zebrafish larvaeDepositorInsertOXER1 (OXER1 Human)
UseUnspecified; Zebrafish expressionTagsmKate2ExpressionMutationPromoterSP6Available sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSP64T-ythdf3-3xflag
Plasmid#164490PurposeExpresses zebrafish ythdf3 with 3x flag tag, for mRNA injectionDepositorInsertythdf3 (ythdf3 Zebrafish)
UseProduction of mrna for zebrafish injection.Tags3x-flagtagExpressionMutationPromoterSP6Available sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
MiniCoopR mitfa:NRAS
Plasmid#118847PurposeExpresses human NRAS Q61R mutant and zebrafish mitfa specifically in zebrafish melanocytesDepositorInsertNRAS Q61R (NRAS Human, Zebrafish)
UseZebrafish expression, tol3TagsExpressionMutationNRAS Q61RPromoterAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
acta1-Cavin4b-Clover
Plasmid#163854PurposeZebrafish muscle specific expression of Cavin4b-Clover. Parton lab clone DQZDepositorInsertCavin4b (cavin4b Zebrafish)
UseZebrafish expressionTagsCloverExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT2h70CRET(#325)
Plasmid#184060Purposecyclofen-inducible CRE activation in zebrafish transient transgenicDepositorInsertCRE-ERT2
UseZebrafish transient transgenesisTagsExpressionMutationERT2: G400V, M543A, L544A, deleted 1-281;PromoterZf-Hsp70-4Available sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
tol2-mpx-Dendra2
Plasmid#29574DepositorInsertmpx-Dendra 2
UseTagsExpressionMutationPromoterAvailable sinceAug. 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-cxadr
Plasmid#192734PurposeGateway entry vector encoding zebrafish cxadrDepositorInsertcxadr (cxadr Zebrafish)
UseGateway entry vectorTagsExpressionMutationPromoterNoneAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-nrip1a
Plasmid#192731PurposeGateway entry vector encoding zebrafish nrip1aDepositorInsertnrip1a (LOC796407 Zebrafish)
UseGateway entry vectorTagsExpressionMutationE662Q; S895P; H967QPromoterNoneAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-btg3
Plasmid#192735PurposeGateway entry vector encoding zebrafish btg3DepositorInsertbtg3 (btg3 Zebrafish)
UseGateway entry vectorTagsExpressionMutationH211YPromoterNoneAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only