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Plasmid#109158PurposeEncodes human full-length HDAC1 to be expressed in a baculovirus/insect cell expression systemDepositorInserthuman full-length HDAC1, sequence-optimized for insect cells (HDAC1 Human)
UseTagsExpressionInsectMutationPromoterp10Available sinceJan. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/DEST/hCx43-E2HA-stop
Plasmid#40909DepositorInsertConnexin 43 internal HA tag (GJA1 Human)
UseTagsExpressionMammalianMutationHA tag inserted in second extracellular loopPromoterCMVAvailable sinceNov. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pIDS-LSD1-C
Plasmid#109160PurposeEncodes a human C-terminal LSD1 fragment to be expressed in a baculovirus/insect cell expression systemDepositorInserthuman C-terminal LSD1, sequence-optimized for insect cells (KDM1A Human)
UseTagsExpressionInsectMutationPromoterp10Available sinceJan. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF1001
Plasmid#143253PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertREST (REST Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAGD335
Plasmid#219499PurposepAGD248 derivative with the retron Eco1 msr, sfGFP flanked by 2 BsaI sites , and the rest of the retron recombineering operon (Eco1 RT, CspRect, MutL-E32K) downstream of the pBad promoterDepositorInsertEco1 msr, sfGFP, Eco1 RT, CspRecT, MutLE32K
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-HA-NLGN4 D396*
Plasmid#59321PurposeExpression plasmid of HA-tagged human Neuroligin 4 harboring a D396* nonsense mutationDepositorInsertNLGN4 D396* (NLGN4X Human)
UseTagsHAExpressionMammalianMutationD396* nonsense mutation, rest of ORF deletedPromoterCAGAvailable sinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJLI-SUP35 (delta)2-5
Plasmid#1138DepositorInsertsup35 delta 2-5 mutant, 5' and 3' flanking regions (SUP35 Budding Yeast)
UseTagsExpressionYeastMutationlast four repeats of sup35 N domain deleted, but …PromoterAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCA-ATG-intron-tdT3Myc
Plasmid#36883DepositorInserttdTomato-3Myc
UseTags3 Myc tagsExpressionMammalianMutationbeta-globin intron containing a loxP site inserte…PromoterCAG (chicken beta actin promoter and CMV enhancer)Available sinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTK-enhNC1.1_M3-MCS-2A-eGFP-SV40pA
Plasmid#110203PurposeRecipient vector for Cranial Neural Crest-specific expression of protein of interestDepositorInsertenhNC1.1_M3-TF_MCS-2A-eGFP-SV40pA
UseTagseGFPExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
Rlucrarr3-Flash5
Plasmid#74133PurposeFlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 263 of Arrestin.DepositorInsertArrestin3
UseTagsRenilla luciferase (rLuc)ExpressionMammalianMutationInsertion of FlAsH motif (CCPGCC) following amino…PromoterAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Rlucrarr3-Flash6
Plasmid#74134PurposeFlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 410 of Arrestin3.DepositorInsertArrestin3
UseTagsRenilla luciferase (rLuc)ExpressionMammalianMutationInsertion of FlAsH motif (CCPGCC) following amino…PromoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Rlucrarr3-Flash1
Plasmid#74129PurposeFlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 40 of Arrestin3.DepositorInsertArrestin3
UseTagsRenilla luciferase (rLuc)ExpressionMammalianMutationInsertion of FlAsH motif (CCPGCC) following amino…PromoterAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
Rlucrarr3-Flash4
Plasmid#74132PurposeFlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 225 of Arrestin3.DepositorInsertArrestin3
UseTagsRenilla luciferase (rLuc)ExpressionMammalianMutationInsertion of FlAsH motif (CCPGCC) following amino…PromoterAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Rlucrarr3-Flash3
Plasmid#74131PurposeFlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 171 of Arrestin3.DepositorInsertArrestin3
UseTagsRenilla luciferase (rLuc)ExpressionMammalianMutationInsertion of FlAsH motif (CCPGCC) following amino…PromoterAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Rlucrarr3-Flash2
Plasmid#74130PurposeFlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 139 of Arrestin3.DepositorInsertArrestin3
UseTagsRenilla luciferase (rLuc)ExpressionMammalianMutationInsertion of FlAsH motif (CCPGCC) following amino…PromoterAvailable sinceApril 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 βarr1 S412D Flag
Plasmid#42196DepositorInsertβ-arrestin 1 S412D (Arrb1 Rat)
UseTagsFlagExpressionMammalianMutationS412DPromoterCMVAvailable sinceJan. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 βarr1 S412A Flag
Plasmid#42195DepositorInsertβ-arrestin 1 S412A (Arrb1 Rat)
UseTagsFlagExpressionMammalianMutationS412APromoterCMVAvailable sinceJan. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBabe.puro.CDK8.KD.flag
Plasmid#19759DepositorInsertcyclin-dependent kinase 8 (CDK8 Human)
UseRetroviralTagsFlagExpressionMammalianMutationD173A (kinase dead)PromoterAvailable sinceDec. 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBabe.puro.CDK8.flag
Plasmid#19758DepositorInsertcyclin-dependent kinase 8 (CDK8 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1.shCDK8
Plasmid#19760DepositorInsertsmall hairpin RNA against CDK8 (CDK8 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only