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pCis mCherry-SS Himar1 A7

Plasmid

#84687

Purpose

Can be used for the transformation of rickettsia, anaplasma and ehrlichia. Uses the Himar1 transposon system, with the A7 allele and both transposase and transposon contained in one plasmid.

Depositor

Ulrike Munderloh

Article

Munderloh et al Intracellular Pathogens II: Rickettsiale

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pSP618

Plasmid

#169235

Purpose

Spear-ATAC lentiviral backbone - includes a mU6-sgRNA cassette with flanking Nextera sequencing adapters

Depositor

William Greenleaf

Article

Pierce et al Nat Commun. 2021 May 20;12(1):2969. doi:

Insert

mU6-sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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LHP306 - Ub - no Lys

Plasmid

#32150

Depositor

Linda Hicke

Article

Terrell et al Mol Cell. 1998 Jan;1(2):193-202.

Insert

synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

No Lys = K6R, K11R, K27R, K29R, K33A, K48R, K63R

Promoter

CUP1

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pJD427

Plasmid

#154371

Purpose

Expresses FKBP12-XB and SspBcore-FRB from constitutive promoters

Depositor

Joseph Davis

Article

Davis et al ACS Chem Biol. 2011 Nov 18;6(11):1205-13

Insert

proB-RBS(B0032)-SspBcore-FRB-terminator(B0011)-proC-RBS(B0032)-FKBP12-sspBXB-terminator(B0011)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pSW609 - N001

Plasmid

#115993

Purpose

intermediate vector for GreenGate cloning method, contains modules from A-F

Depositor

Sebastien Wolf

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

H-A adapter:pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR

Use

Unspecified

Tags

HDEL and SP(ER)

Expression

Mutation

Promoter

Available since

April 25, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-CrkII

Plasmid

#50024

Purpose

pENTR vector for mouse Crk-II

Depositor

Jamie Davies

Article

Cachat et al J Biol Eng. 2014 Nov 19;8(1):26. eCollec

Insert

Crk-II (Crk Mouse)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 10, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO-GRB2-GFP

Plasmid

#86873

Purpose

To express GRB2 with a GFP tag at C terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

GRB2 (GRB2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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BICDN-CIBN

Plasmid

#102253

Purpose

Expresses fusion of dynein adapter BICDN (1-594) and CIB1 (1-170)

Depositor

Bianxiao Cui

Article

Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

Insert

BICDN-CIB1 (CIB1 Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pEF-Bos TRIF Flag

Plasmid

#41550

Depositor

Tom Maniatis, Kate Fitzgerald

Article

Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

Insert

Trif (TICAM1 Human)

Use

Tags

6xHis and Flag

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 26, 2013

Availability

Academic Institutions and Nonprofits only
GFP-BICDN-CIBN

Plasmid

#102254

Purpose

Expresses fusion of dynein adapter BICDN (1-594) with GFP and CIB1 (1-170)

Depositor

Bianxiao Cui

Article

Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

Insert

GFP-BICDN-CIB1 (CIB1 Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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Pu2-3::tag::sl1(G16C)

Plasmid

#79002

Purpose

Expression of exogenous SL1 in multiple tissues of C. elegans

Depositor

Xiao Liu, Ge Zhan

Article

Ma et al Nucleic Acids Res. 2016 Aug 23. pii: gkw

Insert

sls-1.2 (sls-1.2 Nematode)

Use

Tags

illumina-adapter

Expression

Worm

Mutation

G16C

Promoter

F08G2.17

Available since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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Pmyo3::tag:sl1(G16C)

Plasmid

#79003

Purpose

Expression of exogenous SL1 in C. elegans muscle

Depositor

Xiao Liu, Ge Zhan

Article

Ma et al Nucleic Acids Res. 2016 Aug 23. pii: gkw

Insert

sls-1.2 (sls-1.2 Nematode)

Use

Tags

illumina-adapter

Expression

Worm

Mutation

G16C

Promoter

myo-3

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pPPC027.306

Plasmid

#171149

Purpose

Biopterin production pathway with J306 scRNA on pBBR1-GmR plasmid

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Inserts

J306 scRNA
J3-BBa_J23117-GTPCH_J3-BBa_J23117-PTPS_J3-BBa_J23117-SR

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23119 and J3-BBa_J23117

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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Pmyo-3::tag:SL1(G20A)

Plasmid

#81114

Purpose

Expression of exogenous SL1(G20A) in C. elegans muscle

Depositor

Xiao Liu

Article

Ma et al Nucleic Acids Res. 2016 Aug 23. pii: gkw

Insert

sls-1.2 (sls-1.2 Nematode)

Use

Tags

illumina-adapter

Expression

Worm

Mutation

G20A

Promoter

promoter of myo-3

Available since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Lhx2

Plasmid

#74337

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Lhx2. Not for gene expression.

Depositor

Peter Mombaerts

Article

Hirota et al Proc Natl Acad Sci U S A. 2004 Jun 8;101

Insert

LIM homeobox protein 2 (Lhx2 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

May 9, 2016

Availability

Academic Institutions and Nonprofits only
KHBD00176

Plasmid

#34329

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG8376 (ap Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pSAM_AraC

Plasmid

#91569

Purpose

Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.

Depositor

Harry Mobley

Article

Armbruster et al PLoS Pathog. 2017 Jun 14;13(6):e1006434.

Insert

AraC/PBAD

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-STAM1

Plasmid

#164628

Purpose

Expresses STAM1 in mammalian cells with a GFP tag

Depositor

Mark Cookson

Article

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

STAM1 (STAM Human)

Use

Tags

cycle 3 GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Zeo_3xSH2(GRB2)-CTEV-2xHA

Plasmid

#214614

Purpose

adapter for RTK split TEV recruitment assays

Depositor

Michael Wehr

Article

Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

Insert

3xSH2 domains of GRB2-CTEV (GRB2 Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-Lhx2

Plasmid

#61537

Purpose

Expresses Lhx2 in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

Lhx2 (Lhx2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only