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Plasmid#101165PurposeE. coli/S. cerevisiae shuttle vector carrying amd S marker and Spcas9D147Y P411T allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterScTDH3Available sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only
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pSin wPGK-Cre
Plasmid#101242Purposelow-efficiency Cre used to turn on dio YFP/ PSD95-mCherry/ Teal-gephyrin expressionDepositorInsertCre recombinase
UseLentiviralTagsExpressionMutationsee attached filePromoterAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCSN061
Plasmid#101725PurposeSingle copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.DepositorInsertsS. pyogenes Cas9 codon optimized for expression in S. cerevisiae. (NEWENTRY )
KanMX marker expression cassette.
UseCRISPRTagsSV40 NLSExpressionYeastMutationPromoterHeterologous TEF1 promoter from A. gossypii. and …Available sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDD2023
Plasmid#101367PurposeName: pCBR-RBPjkΔB. RBPjk binding site B mutation in pCBR-K14 (pDD2005).DepositorInsertRBPjk binding site B mutation in pCBR-K14 (pDD2005).
UseLuciferaseTagsExpressionMutationRBPjk binding site B mutation in pCBR-K14 (pDD200…PromoterAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDD2022
Plasmid#101366PurposeName: pCBG68-RBPjkΔB. RBPjk binding site B mutation in pCBG68-LANApi (pDD2002).DepositorInsertRBPjk binding site B mutation in pCBG68-LANApi (pDD2002).
UseLuciferaseTagsExpressionMutationRBPjk binding site B mutation in pCBG68-LANApi (p…PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pCSN067
Plasmid#101748PurposeSingle copy yeast plasmid expressing Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1), codon optimized for expression in Saccharomyces cerevisiae.DepositorInsertsCpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1) codon optimized for expression in S. cerevisiae.
KanMX marker expression cassette.
UseCRISPRTagsSV40 NLSExpressionYeastMutationPromoterHeterologous TEF1 promoter from A. gossypii. and …Available sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
EGFP-BAF_L58R
Plasmid#101776PurposeExpresses EGFP tagged mutant BAF (L58R) in human cellsDepositorInsertBAF (BANF1) (BANF1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationL58R mutationPromoterEF1aAvailable sinceJan. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCSN068
Plasmid#101749PurposeSingle copy yeast plasmid expressing Cpf1 from Francisella novicida U112 (FnCpf1), codon optimized for expression in Saccharomyces cerevisiae.DepositorInsertsCpf1 from Francisella novicida U112 (FnCpf1) codon optimized for expression in S. cerevisiae.
KanMX marker expression cassette.
UseCRISPRTagsSV40 NLSExpressionYeastMutationPromoterHeterologous TEF1 promoter from A. gossypii. and …Available sinceJan. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_AHR
Plasmid#101071PurposeDonor vector for 3' FLAG tag of human AHRDepositorInsertAHR homology arms (AHR Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_AHR_1
Plasmid#101076PurposeEncodes gRNA for 3' target of human AHRDepositorInsertgRNA against AHR (AHR Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_AHR_2
Plasmid#101077PurposeEncodes gRNA for 3' target of human AHRDepositorInsertgRNA against AHR (AHR Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDD2000
Plasmid#101197PurposeName: pBluescript-LANApi,K14. Bidirectional reporter driving LANApi side [green luciferase isoform from pCBG68] and K14 side [red luciferase isoform from pCBR] in pBluescript backbone.DepositorInsertLANApi side [green luciferase isoform of pCBG68], K14 side [red luciferase isoform of pCBR]
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
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EGFP-P2A_BAF_G47E
Plasmid#101774PurposeExpresses mutant BAF (G47E) in human cells (with EGFP produced from the same transcript as expression control)DepositorInsertBAF (BANF1) (BANF1 Human)
UseLentiviralTagsEGFP-P2AExpressionMammalianMutationG47E mutationPromoterEF1aAvailable sinceDec. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
EGFP-P2A_BAF
Plasmid#101773PurposeExpresses BAF in human cells (with EGFP produced from the same transcript as expression control)DepositorInsertBAF (BANF1) (BANF1 Human)
UseLentiviralTagsEGFP-P2AExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
EGFP-P2A_BAF_L58R
Plasmid#101775PurposeExpresses mutant BAF (L58R) in human cells (with EGFP produced from the same transcript as expression control)DepositorInsertBAF (BANF1) (BANF1 Human)
UseLentiviralTagsEGFP-P2AExpressionMammalianMutationL58R mutationPromoterEF1aAvailable sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pH3mCHSH2
Plasmid#101053PurposeThis is a Cryptococcus neoformans mCherry expression vector with G418 drug selection marker and C. neoformans SH2 flanking sequences for genome integration.DepositorInsertCryptococcus mCherry expression plasmid
UseTagsExpressionYeastMutationPromoterCryptococcus Histone3 promoterAvailable sinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn1 YFP-CaRhAC
Plasmid#101721PurposeYellow fluorescent protein (YFP) – tagged rhodopsin adenylyl cyclase created from the rhodopsin guanylyl cyclase of Catenaria anguillulae with a neuron-specific promoter. Useful for raising intracelluDepositorInsertYFP CaRhAC
UseAAVTagsYFPExpressionMammalianMutationPromoterhSyn1Available sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn1 ChR2 ET-TC 2A tDimer
Plasmid#101361PurposeHigh efficiency channelrhodopsin, neuron specific promoter, red fluorescent proteinDepositorInsertsChannelrhodopsin-2
synaptophysin-red fluorescent protein
UseAAVTagsExpressionMammalianMutationE123T , T159CPromoterhuman SynapsinAvailable sinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_KMT2B
Plasmid#101070PurposeDonor vector for 3' FLAG tag of human KMT2BDepositorInsertKMT2B homology arms (KMT2B Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only