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Plasmid#166834PurposeExpression and purification of NLRP1 (without the PYD and CARD) from mammalian cellsDepositorInsertNLRP1 (NLRP1 Human)
UseTagsTEV-GFP-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-deltaFlincG
Plasmid#19734DepositorInsertdelta-FlincG
UseTagsHisExpressionMammalianMutationaa 77-356PromoterAvailable sinceMarch 26, 2009AvailabilityAcademic Institutions and Nonprofits only -
pF151 pcDNA3.1(+)SOD1WT
Plasmid#26397DepositorInsertSOD1 (SOD1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 23, 2010AvailabilityAcademic Institutions and Nonprofits only -
pF155 pcDNA3.1(+)SOD1G93A
Plasmid#26401DepositorInsertSOD1 (SOD1 Human)
UseTagsExpressionMammalianMutationG93APromoterAvailable sinceNov. 23, 2010AvailabilityAcademic Institutions and Nonprofits only -
pF152 pcDNA3.1(+)SOD1A4V
Plasmid#26398DepositorInsertSOD1 (SOD1 Human)
UseTagsExpressionMammalianMutationA4VPromoterAvailable sinceNov. 23, 2010AvailabilityAcademic Institutions and Nonprofits only -
pF154 pcDNA3.1(+)SOD1G85R
Plasmid#26400DepositorInsertSOD1 (SOD1 Human)
UseTagsExpressionMammalianMutationG85RPromoterAvailable sinceNov. 23, 2010AvailabilityAcademic Institutions and Nonprofits only -
pF153 pcDNA3.1(+)SOD1G37R
Plasmid#26399DepositorInsertSOD1 (SOD1 Human)
UseTagsExpressionMammalianMutationG37RPromoterAvailable sinceNov. 23, 2010AvailabilityAcademic Institutions and Nonprofits only -
PyronicSF/pcDNA3.1(-)
Plasmid#124812PurposeHighly resposive GFP-based pyruvate nanosensor.DepositorInsertPyronicSF
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_UG7
Plasmid#135990PurposeExpresses UG7 in mammalian cells.DepositorInsertUtrCH
UseTagshelical linker-mEGFPExpressionMammalianMutationPromoterCMV promoterAvailable sinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_UGControl
Plasmid#133875PurposeExpresses UGControl in mammalian cellsDepositorInsertUtrCH (UTRN Human)
UseTagsflexible linker - mEGFPExpressionMammalianMutationPromoterCMV promoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_UG3
Plasmid#133874PurposeExpresses UG3 in mammalian cells.DepositorInsertUtrCH (UTRN Human)
UseTagshelical linker - mEGFPExpressionMammalianMutationPromoterCMV promoterAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
Kv7.1/pcDNA3.1
Plasmid#111452PurposeElectrophysiology in HEK cellDepositorInsertpotassium voltage-gated channel subfamily KQT member 1 isoform 1 [Homo sapiens] (KCNQ1 Human)
UseTags8xHis and EGFPExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-R/G-ICUE
Plasmid#181849PurposeICUE cAMP sensor using sfGFP and mRuby2 as the FRET donor and acceptor.DepositorInsertR/G-ICUE (RAPGEF3 Human)
UseTagsmRuby2 and sfGFPExpressionMammalianMutationGlutamine 122 in Epac1 domain mutated to glutamat…PromoterCMVAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-FluoSTEP-JNKAR
Plasmid#181857PurposeGenetically encoded FRET-based sensor for monitoring JNK kinase activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.DepositorInsertFluoSTEP-JNKAR
UseTagsmRuby2 and sfGFP(1-10)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-FluoSTEP-AktAR
Plasmid#181856PurposeGenetically encoded FRET-based sensor for monitoring Akt activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.DepositorInsertFluoSTEP-AktAR
UseTagsmRuby2 and sfGFP(1-10)ExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-FluoSTEP-EKAR
Plasmid#181858PurposeGenetically encoded FRET-based sensor for monitoring ERK activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.DepositorInsertFluoSTEP-EKAR
UseTagsmRuby2 and sfGFP(1-10)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-FluoSTEP-AKAR
Plasmid#181844PurposeGenetically encoded FRET-based sensor for monitoring PKA activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.DepositorInsertFluoSTEP-AKAR
UseTagsmRuby2 and sfGFP(1-10)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-FluoSTEP-ICUE
Plasmid#181845PurposeGenetically encoded FRET-based sensor for monitoring cAMP dynamics near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.DepositorInsertFluoSTEP-ICUE (RAPGEF3 Human)
UseTagsmRuby2 and sfGFP(1-10)ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MYO10-HMM-Nanotrap
Plasmid#87255PurposeExpression plasmid to perform NanoSPD 2.0 assays in mammalian cells.DepositorInsertMYO10 (Myo10 Mouse)
UseTagsGFP-nanotrapExpressionMammalianMutationMyo10 is truncated to contain aa.1-941PromoterCMVAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
(302) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5)
Plasmid#168192PurposeHA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGT of 4 TPRsDepositorInsertHA-LaG6-(EAAAK)4-OGT(4)
UseTagsHA-TagExpressionMammalianMutationPromoterT7/CMVAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only