Skip to main content
Addgene
Showing: 161 - 180 of 5211 results

  5. pCapV

    Plasmid
    #183028
    Purpose
    the patatin-like phosphodiesterase CapV from the animal commensal strain Escherichia coli ECOR31 cloned in the L-arabinose inducible vector pBAD28
    Depositor
    Ute Romling
    Article
    Li et al NPJ Biofilms Microbiomes. 2022 May 11;8(
    Insert
    patatin-like phospholipase CapV
    Use
    Tags
    No
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD promoter
    Available since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    183028-plasmid-map-sequence-id-361615

    Enlarge

  12. HeFSpCas9

    Plasmid
    #92355
    Purpose
    Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity Cas9-NLS
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A
    Promoter
    Cbh
    Available since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92355-plasmid-map-sequence-id-197617

    Enlarge

  13. pCAH01SpR

    Plasmid
    #128159
    Purpose
    pCAH01 derivative (Ptet inducible promoter) with ahp (kanamycin resistance) CDS replaced with the aadA (streptomycin/spectinomycin resistance) CDS
    Depositor
    Calvin Henard
    Article
    Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Ptet
    Available since
    July 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    128159-plasmid-map-sequence-id-248974

    Enlarge

  17. HeFm2SpCas9

    Plasmid
    #92111
    Purpose
    Expression plasmid for human codon-optimized increased fidelity HeFm2SpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    “Highly enhanced Fidelity” mut2 SpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, K848A, Q926A, R1060A
    Promoter
    Cbh
    Available since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92111-plasmid-map-sequence-id-197616

    Enlarge

Showing: 161 - 180 of 5211 results