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Plasmid#103833PurposeLive cell reader for MS2 loop RNA productionDepositorInsertmKate2-MS2 coat protein (cp )
UseTagsExpressionMammalianMutationMCP: nt 202-240 are missing (13 aa), C242G (A81G)…PromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-2NLS-Cas9-6XMS2
Plasmid#166033PurposeExpression of 6X MS2 stem loops fused SpCas9 mRNA for packaging of SpCas9 mRNA within lentiviral particles.DepositorInsertSpCas9
UseCRISPR and LentiviralTagsExpressionMutationPromoterCMVAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-2NLS-Cas9-6XMS2
Plasmid#166033PurposeExpression of 6X MS2 stem loops fused SpCas9 mRNA for packaging of SpCas9 mRNA within lentiviral particles.DepositorInsertSpCas9
UseCRISPR and LentiviralTagsExpressionMutationPromoterCMVAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pREDIT_Cas9n-MS2-BB_BbsI
Plasmid#164804PurposeREDIT backbone for nickase Cas9n, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9n(D10A)-T2A-EGFPDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS2M-PH-gagpol-D64V
Plasmid#166031PurposeExpression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at N terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol.DepositorInsertMS2-Gag-Pol
UseLentiviralTagsExpressionMutationD64V mutation within the integrase within PolPromoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pREDIT_Cas9n-MS2-BB_BbsI
Plasmid#164804PurposeREDIT backbone for nickase Cas9n, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9n(D10A)-T2A-EGFPDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS2M-PH-gagpol-D64V
Plasmid#166031PurposeExpression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at N terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol.DepositorInsertMS2-Gag-Pol
UseLentiviralTagsExpressionMutationD64V mutation within the integrase within PolPromoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmax pona 12xTRICK 24xMS2SL
Plasmid#64542PurposeTRICK translation biosensor with 12x copies of modified PP7 stem loops and 24x MS2 stem loops.DepositorInsert12x PP7 stem-loops
UseTags24xMS2 stem loops, HA, NLS, and dNP799ExpressionMammalianMutationPromoterponAAvailable sinceMay 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pmax pona 12xTRICK 24xMS2SL
Plasmid#64542PurposeTRICK translation biosensor with 12x copies of modified PP7 stem loops and 24x MS2 stem loops.DepositorInsert12x PP7 stem-loops
UseTags24xMS2 stem loops, HA, NLS, and dNP799ExpressionMammalianMutationPromoterponAAvailable sinceMay 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUBC_stdMCP_serinemod_E488QADAR_p2A_yGFP
Plasmid#154787PurposeExpresses synonymized MS2 coat protein dimer (MCP) fused to hyperactive ADAR catalytic domainDepositorInsertAdenosine deaminse (Adar Fly, Synthetic)
UseLentiviralTagsGFP, V5, and stdMCPExpressionMammalianMutationSynonymized serine to prevent autoediting of ADAR…PromoterUBCAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUBC_stdMCP_serinemod_E488QADAR_p2A_yGFP
Plasmid#154787PurposeExpresses synonymized MS2 coat protein dimer (MCP) fused to hyperactive ADAR catalytic domainDepositorInsertAdenosine deaminse (Adar Fly, Synthetic)
UseLentiviralTagsGFP, V5, and stdMCPExpressionMammalianMutationSynonymized serine to prevent autoediting of ADAR…PromoterUBCAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
phage - ubc - nls - mcp - egfp - BirA*
Plasmid#131134PurposeBirA* expressed as a protein fusion with MS2 coat protein (MCP) monomer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.DepositorInsertNLS-HA-MCP-EGFP-BirA*
UseLentiviralTagsNLS, HAExpressionMammalianMutationBirA* = BIrA >R118GPromoterhuman ubiquitin C (UBC)Available sinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
phage - ubc - nls - mcp - egfp - BirA*
Plasmid#131134PurposeBirA* expressed as a protein fusion with MS2 coat protein (MCP) monomer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.DepositorInsertNLS-HA-MCP-EGFP-BirA*
UseLentiviralTagsNLS, HAExpressionMammalianMutationBirA* = BIrA >R118GPromoterhuman ubiquitin C (UBC)Available sinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
phage - ubc - nls - 2xmcp - egfp - cMycBirA*
Plasmid#131133PurposeBirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.DepositorInsertNLS-HA-2xMCP-EGFP-cMycBirA*
UseLentiviralTagsNLS, HA, cMyc (N-terminal of BirA*)ExpressionMammalianMutationBirA* = BIrA >R118GPromoterhuman ubiquitin C (UBC)Available sinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
phage - ubc - nls - 2xmcp - egfp - cMycBirA*
Plasmid#131133PurposeBirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.DepositorInsertNLS-HA-2xMCP-EGFP-cMycBirA*
UseLentiviralTagsNLS, HA, cMyc (N-terminal of BirA*)ExpressionMammalianMutationBirA* = BIrA >R118GPromoterhuman ubiquitin C (UBC)Available sinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWL078 MCP-2xGFP
Plasmid#197047PurposepCYC-NLS-MCP-2xGFP on pRS305DepositorInsertMCP-2xGFP
UseTagsExpressionYeastMutationPromoterCYC1Available sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWL078 MCP-2xGFP
Plasmid#197047PurposepCYC-NLS-MCP-2xGFP on pRS305DepositorInsertMCP-2xGFP
UseTagsExpressionYeastMutationPromoterCYC1Available sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
yWL268 MCP-GFP
Plasmid#197052PurposepUBC-NLS-HA-2xMCP-2xGFPDepositorInsertpubc-nls-ha-MCP-2xGFP
UseLentiviralTagsExpressionMammalianMutationPromoterubcAvailable sinceMarch 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
yWL268 MCP-GFP
Plasmid#197052PurposepUBC-NLS-HA-2xMCP-2xGFPDepositorInsertpubc-nls-ha-MCP-2xGFP
UseLentiviralTagsExpressionMammalianMutationPromoterubcAvailable sinceMarch 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
yWL234 MCP-GFP-PABPC1*
Plasmid#197054PurposepUBC-NLS-HA-2xMCP-1xGFP-PABPC1*DepositorInsertpubc-nls-ha-MCP-GFP-PABPC1*
UseLentiviralTagsExpressionMammalianMutationPromoterubcAvailable sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only