Addgene

We narrowed to 1,401 results for: loxP

Showing: 161 - 180 of 1401 results

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pMSCV-loxp-dsRed-loxp-3xHA-Puro-WPRE

Plasmid

#32703

Depositor

Hans Clevers

Article

Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

Type

Empty backbone

Use

Cre/Lox and Retroviral

Tags

3x HA tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pJFRC172-10XUAS-loxP>-dSTOP-loxP>-myr::GFP

Plasmid

#32145

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

loxP> STOP STOP loxP>

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

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ACTB-PQR-RFPnols-PQR-loxP-Zeo-loxP

Plasmid

#121448

Purpose

To make a stable human recombinant cell line; see right thumbnail map image for PQR annotations

Depositor

Brian Chen

Article

Lo et al Biotechniques. 2017 Apr 1;62(4):165-174.

Insert

RFPnols-PQR-ZeocinR (ACTB Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pDZ264 (pKAN tdtomato cyc loxP KAN loxP)

Plasmid

#35193

Depositor

Robert Singer

Article

Larson et al Science. 2011 Apr 22;332(6028):475-8.

Insert

tdtomato cyc loxP KAN loxP

Use

Tags

Expression

Mutation

Promoter

Available since

March 5, 2012

Availability

Academic Institutions and Nonprofits only

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pALS186 (ttTi5605-MCS loxP::C.br.unc-119(+)::loxP)

Plasmid

#129539

Purpose

modified pCFJ151 for C. elegans mosSCI at ttTi5605 or compatible sites; floxed C. br. unc-119(+) selection

Depositor

Arneet Saltzman

Article

Aram et al MicroPubl Biol. 2019 Aug 27;2019. doi: 1

Type

Empty backbone

Use

Cre/Lox; Mos1-mediated single copy insertion (mos…

Tags

Expression

Worm

Mutation

Promoter

Available since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pK029.CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE (Supernova)

Plasmid

#69138

Purpose

For the Supernova, a series of vector systems for single-cell labeling and gene function sudies in vivo

Depositor

Takuji Iwasato

Article

Mizuno et al Neuron. 2014 Apr 16;82(2):365-79. doi: 1

Insert

CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE

Use

Tags

none

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova)

Plasmid

#85006

Purpose

For Cre/loxP-based Supernova-GFP, pK038 should be used with pK031. The GFP fragment of pK038 can be replaced with another gene by using SalI and EcoRV sites.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

EGFP-ires-tTA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pK098.CAG-loxP-stop-loxP-nlstagRFP-ires-tTA-WPRE (Supernova)

Plasmid

#85179

Purpose

For nucleus-specific RFP labeling in single cells, pK098 should be used with pK031.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

nuclear localization signal (nls) tagRFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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TLCV2-LoxP

Plasmid

#127098

Purpose

LentiCRISPR v2 was modified into an all-in-one dox inducible system.

Depositor

Adam Karpf

Insert

Cas9-2A-eGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pX330.LoxP

Plasmid

#66586

Purpose

sgRNA for LSL

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

LoxP sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only
pAV113.loxP

Plasmid

#63186

Purpose

pRS413 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pAV115.loxP

Plasmid

#63187

Purpose

pRS415 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pAV116.loxP

Plasmid

#63188

Purpose

pRS416 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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loxP-T2A-Myr-DeadCasp8-ERT2-rox-FNF-loxP-T2A-Myr-Casp8-ERT2-rox

Plasmid

#182360

Purpose

Shuttle vector for T2A-Myr-DeadCasp8-ERT2 and T2A-Myr-Casp8-ERT2

Depositor

Mario Capecchi

Insert

Myr-DeadCasp8-ERT2 and Myr-Casp8-ERT2

Use

Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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CAG-loxP-3xpA-loxP-H2B-sfGFP+pCI syntron-T2A-tdTomato+RbG and HsbG syntrons

Plasmid

#172480

Purpose

H2B-sfGFP with a synthetic intron from the pCI expression vector, T2A, and tdTomato with synthetic introns derived from the rabbit beta-globin gene and the human beta-globin gene

Depositor

Mario Capecchi

Insert

H2B-sfGFP-T2A-tdTomato

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean

Plasmid

#24334

Depositor

Didier Stainier

Article

Hesselson et al Proc Natl Acad Sci U S A. 2009 Sep 1. 10

Insert

H2B

Use

Cre/Lox

Tags

GFP

Expression

Worm

Mutation

mCherry driven by an HSP70 promoterCerulean drive…

Promoter

Available since

April 27, 2010

Availability

Academic Institutions and Nonprofits only

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pK237.U6-Chimeric-CAG-loxP-stop-loxP-hspCas9 (Supernova)

Plasmid

#85041

Purpose

This plasmid should be used for Cre/loxP-based Supernova-CRISPR/Cas9.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

loxP-stop-loxP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pTol2p2A--3mnx1:loxP-mTagBFP2-stop-loxP-mCherry-NS-MCS

Plasmid

#78251

Purpose

Can be used to subclone in tagged gene of interest into inducible motor neuron expression vector.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Inserts

-3mnx1 (mnx1 Zebrafish)
-loxP-mTagBFP2-stop-
mCherry-NS-MCS

Use

Zebrafish motor neuron inducible expression

Tags

Expression

Mutation

Promoter

-3mnx1

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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PBRPyCAG-LoxP-ERAS-STOPcassette-Loxp-DsRed-IRES-PURO

Plasmid

#52419

Purpose

Constitutive mammalian expression of ERAS. Upon CRE mediated deletion of ERAS insert, dsRED expression markers is truned on due to concomittant removal of stop cassette.

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Insert

ERAS (ERAS Human)

Use

Tags

Expression

Mutation

Promoter

CAGGS

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pBeloBac11loxP2272

Plasmid

#60342

Purpose

This is a BAC vector that contains both wt and mutant loxP sites.

Depositor

Jon Coren

Article

Coren et al BMC Res Notes. 2017 Jul 28;10(1):344. do

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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