Skip to main content
Addgene
Showing: 161 - 180 of 385 results

  6. pAAV-CMV-FRT-myrSNAP-2A-H2BeGFP-WPRE

    Plasmid
    #102986
    Purpose
    AAV expressing membrane-targeted SNAP protein and nuclear-targeted eGFP after FLP-mediated recombination
    Depositor
    Marco Tripodi
    Article
    Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306
    Inserts
    membrane-targeted SNAP protein after FLP-mediated recombination
    Nuclear-targeted eGFP after FLP-mediated recombination
    Use
    AAV; Adeno associated viral vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    102986-plasmid-map-sequence-id-200006

    Enlarge

  9. CAG-loxP-3xpA-H2BsfGFP-tdTomato - T2A V XE

    Plasmid
    #113843
    Purpose
    Histone H2B-superfolder GFP and tdTomato linked by T2A (V linker)
    Depositor
    Mario Capecchi
    Insert
    H2B-sfGFP-T2A-tdTomato
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113843-plasmid-map-sequence-id-223854

    Enlarge

  10. CAG-loxP-3xpA-H2BsfGFP-tdTomato - T2A Q XE

    Plasmid
    #113842
    Purpose
    Histone H2B-superfolder GFP and tdTomato linked by T2A (Q linker)
    Depositor
    Mario Capecchi
    Insert
    H2B-sfGFP-T2A-tdTomato
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113842-plasmid-map-sequence-id-223654

    Enlarge

  14. pX458-Ef1a-dCas9-VPR-H2B-GFP (CRISPRa)

    Plasmid
    #175571
    Purpose
    Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation
    Depositor
    Joseph Brzezinski
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    175571-plasmid-map-sequence-id-344994

    Enlarge

  15. hEF1α-H2B-mOrange2-IRES-EGFP PGK-Puromycin

    Plasmid
    #99277
    Purpose
    Dual fluorescent reporter - constitutive expression of mOrange2 (549/565) localized in the nucleus, EGFP (488/507) untargeted and visualized in the cytoplasm, and Puromycin resistance
    Depositor
    Jordan Miller
    Article
    Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/
    Inserts
    H2B-mOrange2-IRES-EGFP
    Puro
    Use
    Lentiviral
    Tags
    EGFP, H2B, IRES, PGK, and mOrange2
    Expression
    Mutation
    Promoter
    EF1 alpha-human and PGK-mouse
    Available since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99277-plasmid-map-sequence-id-197594

    Enlarge

Showing: 161 - 180 of 385 results