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Plasmid#177925PurposeExpresses optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast MYO2 promoterDepositorArticleInsertOptimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast MYO2 promoter
UseTagsExpressionBacterial and YeastMutationV9I; F67S; G72P, F86Y; R171KPromoterMYO2Available sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 VDAC1-TC
Plasmid#211733PurposeExpresses tetracysteine tagged VDAC1 in mammalian cells. TC-tag is attached to the C-terminus of VDAC1.DepositorInsertVoltage-dependent anion channel 1 (VDAC1 )
UseTagsTetracysteineExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVY-16 TC-TEM1
Plasmid#194630Purposeantibiotic resistance enzyme with tetracysteine tag for visualization of enzymeDepositorInserttetracysteine-TEM1
UseTagsMGCCPGCCGGAExpressionBacterialMutationPromoterpBADAvailable sinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVY-14 TC-CAT
Plasmid#194628Purposeantibiotic resistance enzyme with tetracysteine tag for visualization of enzymeDepositorInserttetracysteine-CAT
UseTagsMGCCPGCCGGAExpressionBacterialMutationPromoterT7Available sinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVY-17 TC-TEM1-tag3
Plasmid#194631Purposeantibiotic resistance enzyme with cationic peptide tag and tetracysteine tagDepositorInserttetracysteine-TEM1-GGSKKRKKRGGSKKRKKRGGSKKRKKR
UseTagsGGSKKRKKRGGSKKRKKRGGSKKRKKR and MGCCPGCCGGAExpressionBacterialMutationPromoterpBADAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVY-19 TC-sfGFP-tag3
Plasmid#194633Purposefluorescent protein with cationic peptide tag and tetracysteine tagDepositorInserttetracysteine-sfGFP-K(GGSKKRKKR)3
UseTagsKGGSKKRKKRGGSKKRKKRGGSKKRKKR and MGCCPGCCGGAExpressionBacterialMutationPromoterT7Available sinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVY-15 TC-CAT-tag3
Plasmid#194629Purposeantibiotic resistance enzyme with cationic peptide tag and tetracysteine tagDepositorInserttetracysteine-CAT-(GGSKKRKKR)3
UseTagsGGSKKRKKRGGSKKRKKRGGSKKRKKR and MGCCPGCCGGAExpressionBacterialMutationPromoterT7Available sinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIN1_GGGSx3_mScarlet
Plasmid#182936PurposeC-terminal mScarlet with flexible linker (GGGGS)x3DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Notch1 ICv-6MT
Plasmid#41730DepositorInsertNotch-1 Intracellular Domain (Notch1 Mouse)
UseTags6xMycExpressionMammalianMutationContains murine Notch-1 protein beginning at Vali…PromoterAvailable sinceDec. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLY232
Plasmid#184149Purposeshort tracrRNA generator with WT anti-repeat regionDepositorInserts-tracrRNA-WT
UseSynthetic BiologyTagsExpressionBacterialMutationPromoteraraBAD promoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEK22
Plasmid#210588PurposeReporter gene expression in B. dendrobatidisDepositorInsertBdH3pro-Hshph-G4S-nanoluc-ScADH1ter
UseLuciferaseTagsNanoLuc luciferaseExpressionBacterial and YeastMutationPromoterBdH3proAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBC114
Plasmid#202276PurposeLevel 2 for strain-specific barcode DNA tag with bc141 through Tn7 bacterial insertion, Tetracyline selectable marker and far-red-FP fluorescent marker mPlumDepositorInsertbc141
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCOM-CD63-COM
Plasmid#138350PurposeExpressing COM-CD63-COM fusion protein in mammalian cellsDepositorInsertCOM-CD63-COM fusion protein
UseCRISPR and LentiviralTagsCOM is fused to the N and C termini of human CD63ExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CD81-(TEV)-Cre
Plasmid#167939Purposehuman CD81-TEV cleavage site-CreDepositorInserthuman CD81 (CD81 Human)
UseTagsCre recombinase and TEV protease cleavage siteExpressionMammalianMutationPromoterCMVAvailable sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CD81-(TEV)-tTA
Plasmid#162598Purposehuman CD81-TEV cleavage site-tTADepositorInserthuman CD81 (CD81 Human)
UseTagsTEV protease cleavage site and tTAExpressionMammalianMutationPromoterCMV promoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAOJ15
Plasmid#91567PurposeBroad host range allelic exchange suicide vector for bacteria - Tse2 toxin driven by anhydrotetracycline inducible promoterDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterTetracycline promoterAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CD9-(TEV)-tTA
Plasmid#162596Purposehuman CD9-TEV cleavage site-tTADepositorInserthuman CD9 (CD9 Human)
UseTagsTEV protease cleavage site and tTAExpressionMammalianMutationPromoterCMV promoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV/N3IC-HA
Plasmid#47618Purposein vitro transcription and translation of HA-tagged Notch3 Intracellular domainDepositorInsertNotch3 Intracellular domain (Notch3 Mouse)
UseIn vitro transcription and translationTagsHAExpressionMammalianMutationcodons 1664 (Met) to 2318 (Ala)PromoterCMVAvailable sinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEK19
Plasmid#210578PurposeReporter gene expression in B. dendrobatidisDepositorInsertBdBIP1pro-Hshph-G4S-nanoluc-ScADH1ter
UseLuciferaseTagsNanoLuc luciferaseExpressionBacterial and YeastMutationPromoterBdBIP1proAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CD63-(TEV)-tTA
Plasmid#162597Purposehuman CD63-TEV cleavage site-tTADepositorInserthuman CD63 (CD63 Human)
UseTagsTEV protease cleavage site and tTAExpressionMammalianMutationPromoterCMV promoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only