Addgene

We narrowed to 16,158 results for: IGH@;

Showing: 161 - 180 of 16158 results

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pNL-EGFP/TRETightdU3

Plasmid

#18660

Depositor

Jakob Reiser

Article

Pluta et al J Gene Med. 2005 Jun . 7(6):803-17.

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 8, 2008

Availability

Academic Institutions and Nonprofits only

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pRIV-Gal4DBD:2xnMagHigh1

Plasmid

#182031

Purpose

Donor plasmid for conversion of KO attP Drosophila lines into ShineGal4 drivers

Depositor

Jean-Paul Vincent

Article

di Pietro et al Dev Cell. 2021 Dec 20;56(24):3393-3404.e

Insert

Gal4DBD2xHighMag1-p103'UTR

Use

Donor plasmid

Tags

Expression

Insect

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-Tight-Puro_Myt1

Plasmid

#64845

Purpose

A lentiviral vector for the expression of mouse Myt1 under doxycyline control

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

Myt1 (Myt1 Mouse)

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

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pJL1-TTlight-4xDQNAT

Plasmid

#128394

Purpose

In vitro expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Tetanus toxin light chain domain

Use

Tags

4xDQNAT glycosylation tag and 6xHis tag

Expression

Bacterial

Mutation

Inactivating E234A mutation

Promoter

T7

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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TALED_Right-stuffer-1397C-8e

Plasmid

#187170

Purpose

original vector to make all constructs

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

Right-stuffer-1397C-8e

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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TALED_Right-stuffer-8e-E1347A

Plasmid

#187166

Purpose

original vector to make all constructs

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

Right-stuffer-8e-E1347A

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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TALED_Right-stuffer-E1347A

Plasmid

#187164

Purpose

original vector to make all constructs

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

Right-stuffer-E1347A

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/hpa5(aIIB_thigh)

Plasmid

#59971

Purpose

integrin a5 chimeric headpiece expression (a5 beta-propeller domain and aIIB thigh domain)

Depositor

Timothy Springer

Insert

human integrin a5 beta-propeller domain fused with human integrin aIIB thigh domain (ITGA5, ITGA2B Human)

Use

Tags

Acid coiled-coil and StrepII tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2014

Availability

Academic Institutions and Nonprofits only
Chicken ArcLight S174

Plasmid

#53567

Purpose

Genetically encoded voltage sensor ArcLight with improved kinetics

Depositor

Vincent Pieribone

Article

Han et al PLoS One. 2013 Nov 27;8(11):e81295. doi:

Insert

Chicken ArcLight-S174

Use

Tags

Expression

Mammalian

Mutation

Gg-VSP contains an R153Q mutation and an amino ac…

Promoter

CMV

Available since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-H2-18 Light chain

Plasmid

#216308

Purpose

Expresses anti-HER2 H2-18 Light chain in mammalian cells. To be paired with H2-18 heavy chain to form the anti-HER2 H2-18 Fab

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

H2-18 Light chain

Use

Affinity Reagent/ Antibody

Tags

Signal peptide

Expression

Mammalian

Mutation

Promoter

T7 promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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T7RNAP Cyslight V597C

Plasmid

#205080

Purpose

T7RNAP protein expression

Depositor

Smita Patel

Insert

T7RNAP Cyslight V597C (T7p07 Bacteriophage T7)

Use

Tags

Expression

Bacterial

Mutation

C125S, C347S, C510S, C515S, C530S, C723S V597C

Promoter

Available since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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T7RNAP Cyslight V166C

Plasmid

#205079

Purpose

T7RNAP protein expression

Depositor

Smita Patel

Insert

T7RNAP Cyslight V166C (T7p07 Bacteriophage T7)

Use

Tags

Expression

Bacterial

Mutation

C125S, C347S, C510S, C515S, C530S, C723S V166C

Promoter

Available since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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Cox1-FusXTBE-RightTALE

Plasmid

#196503

Purpose

Expresses right-side TALE–DddAtox C term half cox1-FusXTBE in zebrafish

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

IDH2 MTS-cox1 right TALE-G1397 DddA-C-UGI-SV40 polyA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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Cox3-FusXTBE-RightTALE

Plasmid

#196505

Purpose

Expresses right-side TALE–DddAtox C term half cox3-FusXTBE in zebrafish

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

IDH2 MTS-cox3 right TALE-G1397 DddA-C-UGI-SV40 polyA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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tl1-FusXTBE-RightTALE

Plasmid

#196507

Purpose

Expresses left-side TALE–DddAtox N term half tl1-FusXTBE in zebrafish

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

IDH2 MTS-tl1 right TALE-G1397 DddA-C-UGI-SV40 polyA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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ND6-L1397-N_Q1310A_right

Plasmid

#187849

Purpose

expresses the right-side half ND6-Q1310A in mammalian cells

Depositor

Chengqi Yi

Article

Lei et al Nature. 2022 May 12. pii: 10.1038/s41586

Insert

COX8A MTS–3xFLAG–ND6 right TALE–G1397 DddA-C–UGI–ATP5B 3'UTR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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hp-50%GC-Right-half-120bp

Plasmid

#169205

Purpose

Force-activating hairpin for 3'-to-5' helicase

Depositor

Tom Perkins

Article

Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

Insert

50%GC-Right-half-120bp-hairpin

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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hp-50-0-50%GC-Right-half-120bp

Plasmid

#169207

Purpose

50-0-50%GC-Right-half-120bp-hairpin

Depositor

Tom Perkins

Article

Taylor et al Methods Mol Biol. 2022;2478:273-312. doi

Insert

50-0-50%GC-Right-half-120bp-hairpin

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD/His-D-iLight

Plasmid

#170270

Purpose

Expression of iLight with 6xHis N-terminal tag in bacteria.

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Insert

iLight

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

See Comments

Promoter

araBAD

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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IgH intron enhancer

Plasmid

#127247

Purpose

To maximize gene expression in B cells

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

heavy chain enhancer

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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