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Showing: 161 - 180 of 240 results

  10. pJEC581

    Plasmid
    #174369
    Purpose
    RFP reporter used to evaluate CRISPRa in bacteria, contains gRNA target sites every 10 bp upstream of the promoter.
    Depositor
    James Chappell
    Article
    Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793
    Insert
    RFP with PAM rich sequence upstream promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23117
    Available since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    174369-plasmid-map-sequence-id-345702

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  14. pJEC819

    Plasmid
    #190815
    Purpose
    CRISPRa backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.
    Depositor
    James Chappell
    Article
    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    dCas9-XTEN-rpoA
    Use
    CRISPR and Synthetic Biology
    Tags
    XTEN (linker), followed by the N-terminal domain …
    Expression
    Mutation
    Promoter
    SP30
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    190815-plasmid-map-sequence-id-373717

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  19. pJEC626

    Plasmid
    #174367
    Purpose
    Activation domain for modular CRISPRa. AD-SYNZIP. Expresses alphaNTD from the E coli RNAP with a SYNZIP17 domain fused to the N terminus.
    Depositor
    James Chappell
    Article
    Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793
    Insert
    Alpha N-terminal domain from the E. coli RNA polymerase fused to SYNZIP17
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23106
    Available since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    174367-plasmid-map-sequence-id-345703

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Showing: 161 - 180 of 240 results