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Plasmid#80059PurposeMCS for cloning promoter to drive HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
mycBioID-pBabePuro-myc-BirA*HRASG12V
Plasmid#122214PurposeStably expresses myc-BirA* fused to HRASG12V in mammalian cellsDepositorInsertHRASG12V (HRAS Human)
UseRetroviralTagsmyc-BirA*ExpressionMammalianMutationchanged G12 to VPromoterAvailable sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3/flag-pBirA(1-G78)-FRB
Plasmid#154447Purposeexpresses pBirA(1-G78)-FRB/biochemical assayDepositorInsertflag-pBirA(1-G78)-FRB
UseTagsExpressionMammalianMutationPromoterT7 promoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMT-zic2a-NLS-BirA-2A-mCherry_Ras
Plasmid#80065PurposeZic2a promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialMutationPromoterZic2a enhancer driving c-fos promoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
R4pGWB601_UBQ10p-BirA(R118G)-NES-YFP
Plasmid#127363PurposeBinary vector for expressing cytosolic BirA* (R118G)-YFP under the UBQ10 promoter in plantsDepositorInsertBirA* (R118G mutant)
UseTagsGS linker, NES, V5, and YFPExpressionPlantMutationR118GPromoterArabidopsis UBQ10 promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MCS-BirA(R118G)-HA-MACROD1
Plasmid#172581PurposeFor transient expression of C-terminal BirA-tagged MACROD1DepositorInsertMACROD1 (MACROD1 Human)
UseTagsBirA(R118G)-HAExpressionMammalianMutationPromoterAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMT-myl7-NLS-BirA-2A-mCherry_Ras
Plasmid#80061PurposeMyl7 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialMutationPromoterMyl7 promoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLPC-N-Myc-Flag-BirA-hPOT1
Plasmid#166409Purposeexpress BirA-hPOT1 in mammalian cellsDepositorInserthPOT1 (POT1 Human)
UseTagsBirA, flag, and mycExpressionMammalianMutationPromotercmvAvailable sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXL-CAG-mBirA272-NRX3b D137A
Plasmid#43922DepositorInsertBirA272-NRX3b (birA Synthetic, Human)
UseTags2XFLAGExpressionMammalianMutationD137APromoterCAGAvailable sinceApril 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
mycBioID-pBabePuro-myc-BirA*NRASG12V
Plasmid#122212PurposeStably expresses myc-BirA* fused to NRASG12V in mammalian cellsDepositorInsertNRASG12V (NRAS Human)
UseRetroviralTagsmyc-BirA*ExpressionMammalianMutationchanged G12 to VPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS416-ADH1p-BirA-FRB-HA3
Plasmid#227984PurposeExpresses BirA-FRB fusion from ADH1 promoter; yeast BioIDDepositorInsertBirA-FRB
UseTagsHA tagExpressionYeastMutationPromoterADH1Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416-ADH1p-BirA-FKBP-HA3
Plasmid#227986PurposeExpresses BirA-FKBP fusion from ADH1 promoter; yeast BioIDDepositorInsertBirA-FKBP
UseTagsHA tagExpressionYeastMutationPromoterADH1Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-myc-BirA (JDW 1210)
Plasmid#224509PurposeGateway compatible middle entry clone containing a Myc tagged BirADepositorInsertMyc-BirA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Myc-BirA*-RNF8-C403S
Plasmid#221685PurposeMammalian expression of Myc-tagged BirA*-RNF8 (C403S) fusion protein for BioIDDepositorInsertRNF8 (RNF8 Human)
UseTagsExpressionMammalianMutationC403SPromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Myc-BirA*-RNF8-R42A
Plasmid#221684PurposeMammalian expression of Myc-tagged BirA*-RNF8 (R42A) fusion protein for BioIDDepositorInsertRNF8 (RNF8 Human)
UseTagsExpressionMammalianMutationR42APromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-N-FLAG-hBirA*-TLK1-WT
Plasmid#218834PurposeExpresses N-terminal BirA*-tagged human wild type TLK1 variant 5 for BioID experiments.DepositorInsertTLK1 (TLK1 Human)
UseTagsFLAG-BirA*ExpressionMammalianMutationPromoterCMVAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
3'-BirA*-FLAG minimal CMV pCDNA5
Plasmid#212047PurposeLR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locusDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
5'-BirA*-FLAG minimal CMV pCDNA5
Plasmid#212046PurposeLR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locusDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLIK BirA* 3xFLAG-NUP37 zeo
Plasmid#192333PurposeLentiviral expression vector for an inducible 3xFLAG-BirA 3xFLAG-NUP37DepositorInsertBirA*-3xFLAG-NUP37 (NUP37 Human)
UseLentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLIK BirA* 3xFLAG-CSE1L zeo
Plasmid#192334PurposeLentiviral expression vector for an inducible 3xFLAG-BirA 3xFLAG-CSE1LDepositorInsertBirA*-3xFLAG-CSE1L (CSE1L Human)
UseLentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only