-
Plasmid#47050PurposeBglBrick plasmid (=pBbB8k-P450) coding for a cytochrome P450 and its associated reductases to oxidize limonene to perillyl alcohol in E. coliDepositorInsertahpG-ahpH-ahpI coding for cytochrome P450 (CYP153A6) and its associated ferredoxin and ferredoxin reductase
UseSynthetic Biology; BglbrickTagsExpressionBacterialMutationPromoterPbadAvailable sinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-VdC9BV
Plasmid#62195PurposeExpresses RNA-Guided, Nuclease-Inactive VP64:dCas9-BFP:VP64—VdC9BV—Fusion Protein to Enable Transactivation of Endogenous GenesDepositorInsertVP64dCas9BFPVP64
UseLentiviralTagsTwo VP64s tagged to dCas9 fused to BFPExpressionMammalianMutationD10A H840A (catalytically inactive) Cas9 (dCas9)PromoterAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSR357.24
Plasmid#125109PurposeExpression of narL(1-159aa)-ydfI(153-213aa) chimera under PLtetO-1, output Promoter PydfJ115DepositorInsertsnarL(REC)-ydfI(DBD)159
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR348
Plasmid#124713PurposeExpression of narX under PtacDepositorUseSynthetic BiologyTagsExpressionBacterialMutationS193* - LacI is truncated and likely deactivated.…PromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRB117.2
Plasmid#181949PurposeaTc-inducible expression of NarL with N-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuSDepositorInsertUseTagsmNeonGreenExpressionBacterialMutationSee Depositor Comments BelowPromotermNG-NarL-PLtetO-1; mCherry-PdcuS; tetR-J23106Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSR358.4
Plasmid#125114PurposeExpression of narL(1-159aa)-liaR(154-211aa) chimera under PLtetO-1, output Promoter PliaI121DepositorInsertsnarL(REC)-liaR(DBD)159
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR357.10
Plasmid#125106PurposeExpression of narL(1-131aa)-ydfI(126-213aa) chimera under PLtetO-1, output Promoter PydfJ115DepositorInsertsnarL(REC)-ydfI(DBD)131
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
pSR284
Plasmid#125093PurposeExpression of ompR(1-122aa)-manR(120-234aa) chimera under PLtetO-1, output Promoter PmntCAB143DepositorInsertsompR(REC)-manR(DBD)122
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR241
Plasmid#125091PurposeExpression of ompR(1-137aa)-yxdJ(132-229aa) chimera under PLtetO-1, output Promoter PyxdL181DepositorInsertsompR(REC)-yxdJ(DBD)137
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD279
Plasmid#125099PurposeExpression of SO_4388(1-130aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110DepositorInsertsSO_4388(REC)-psdR(DBD)137
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR357.9
Plasmid#125105PurposeExpression of narL(1-129aa)-ydfI(124-213aa) chimera under PLtetO-1, output Promoter PydfJ115DepositorInsertsnarL(REC)-ydfI(DBD)129
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRB113.2
Plasmid#181948PurposeaTc-inducible expression of NarL with C-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuSDepositorInsertUseTagsmNeonGreenExpressionBacterialMutationPromoterNarL-mNG-PLtetO-1; mCherry-PdcuS; tetR-J23106Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEG03
Plasmid#73841PurposeEncodes PhyB917 with a C-terminal histidine tag, for protein expression in Dicty.DepositorInsertPhyB(1-917) with C-term his tag (PIL2 Mustard Weed)
UseDictyostelium discoideum expressionTags6x his tagExpressionMutationPromoterAvailable sinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKD265
Plasmid#125095PurposeExpression of torR(1-134aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110DepositorInsertstorR(REC)-psdR(DBD)137
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR322
Plasmid#125116PurposeExpression of uhpA(1-123aa)-ydfI(126-213aa), narL131 chimera under PLtetO-1, output Promoter PydfJ115DepositorInsertsuhpA(REC)-ydfI(DBD)131
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR40.15
Plasmid#125082PurposeExpression of ompR(1-137aa)-ccaR(127-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172DepositorInsertsompR(REC)-ccaR(DBD)137
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR40.29
Plasmid#125088PurposeExpression of ompR(1-139aa)-ccaR(129-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172DepositorInsertsompR(REC)-ccaR(DBD)139
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRB116.2
Plasmid#181946PurposeaTc-inducible expression of TorR with C-terminal mNeonGreen fusion. Also contains mCherry under TorR-controlled promoter PtorCAD129DepositorInsertUseTagsmNeonGreenExpressionBacterialMutationPromoterTorR-mNG-PLtetO-1; mCherry-PtorCAD129; tetR-J23106Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRB120.2
Plasmid#181947PurposeaTc-inducible expression of TorR with N-terminal mNeonGreen fusion. Also contains mCherry under TorR-controlled promoter PtorCAD129DepositorInsertUseTagsmNeonGreenExpressionBacterialMutationPromotermNG-TorR-PLtetO-1; mCherry-PtorCAD129; tetR-J23106Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRB133.2
Plasmid#181950PurposeaTc-inducible expression of PhoP with C-terminal mNeonGreen fusion. Also contains mCherry under PhoP-controlled promoter PvirKDepositorInsertPhoP-Salmonella enterica subsp. enterica serovar Typhimurium (AX04_RS23485 Synthetic, PhoP-Salmonella enterica subsp. enterica serovar Typhimurium)
UseTagsmNeonGreenExpressionBacterialMutationPromoterPhoP-mNG-PLtetO-1; mCherry-PvirK; tetR-J23106Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_HLA-cmyc-GRM3 C127A TMD Y616M
Plasmid#140454PurposeMammalian expression plasmid for c-myc-tagged mGluR3 C127A and Y616M mutantDepositorInsertGRM3 (GRM3 Human)
UseTagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationCysteine 127 to alanine, tyrosine 616 to methioni…PromoterAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_HLA-cmyc-GRM3 C127A TMD L624N
Plasmid#140455PurposeMammalian expression plasmid for c-myc-tagged mGluR3 C127A and L624N mutantDepositorInsertGRM3 (GRM3 Human)
UseTagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationCysteine 127 to alanine, leucine 624 to asparaginePromoterAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_HLA-cmyc-GRM3 C127A TMD A634N
Plasmid#140456PurposeMammalian expression plasmid for c-myc-tagged mGluR3 C127A and A634N mutantDepositorInsertGRM3 (GRM3 Human)
UseTagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationCysteine 127 to alanine, alanine 634 to asparaginePromoterAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4_HLA-flag-GRM3 C127A-VN
Plasmid#140449PurposeMammalian expression plasmid for FLAG-tagged mGluR3 fused to the N-terminal half of split fluorescent Venus (VN)DepositorInsertGRM3 (GRM3 Human)
UseTagsFLAG tag, Signal/leader sequence from HLA class I…ExpressionMammalianMutationCysteine 127 to alaninePromoterAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4_HLA-myc-GRM3 C127A-VC
Plasmid#140450PurposeMammalian expression plasmid for c-myc-tagged mGluR3 fused to the C-terminal half of split fluorescent Venus (VC)DepositorInsertGRM3 (GRM3 Human)
UseTagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationCysteine 127 to alaninePromoterAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_HLA-cmyc-GRM3 C127A TMD I578A
Plasmid#140452PurposeMammalian expression plasmid for c-myc-tagged mGluR3 C127A and I578A mutantDepositorInsertGRM3 (GRM3 Human)
UseTagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationCysteine 127 to alanine, isoluecine 578 to alaninePromoterAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_HLA-cmyc-GRM3 C127A TMD F588V
Plasmid#140453PurposeMammalian expression plasmid for c-myc-tagged mGluR3 C127A and F588V mutantDepositorInsertGRM3 (GRM3 Human)
UseTagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationCysteine 127 to alanine, phenylalanine 588 to val…PromoterAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKD277
Plasmid#125098PurposeExpression of SO_2193(1-134aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110DepositorInsertsSO_2193(REC)-psdR(DBD)137
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR357.20
Plasmid#125108PurposeExpression of narL(1-154aa)-ydfI(148-213aa) chimera under PLtetO-1, output Promoter PydfJ115DepositorInsertsnarL(REC)-ydfI(DBD)154
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR322.2
Plasmid#125117PurposeExpression of uhpA(1-118aa)-ydfI(121-213aa), narL126 chimera under PLtetO-1, output Promoter PydfJ115DepositorInsertsuhpA(REC)-ydfI(DBD)126
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR359.4
Plasmid#125115PurposeExpression of narL(1-159aa)-uhpA(142-129aa) chimera under PLtetO-1, output Promoter PuhpT99DepositorInsertsnarL(REC)-uhpA(DBD)159
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR40.16
Plasmid#125083PurposeExpression of ompR(1-140aa)-ccaR(130-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172DepositorInsertsompR(REC)-ccaR(DBD)140
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD287
Plasmid#125103PurposeExpression of SO_4637(1-127aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110DepositorInsertsSO_4637(REC)-psdR(DBD)137
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD284
Plasmid#125073PurposeExpression of SO_4488 under PtacDepositorUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD278
Plasmid#125070PurposeExpression of SO_2192 under PtacDepositorUseSynthetic BiologyTagsExpressionBacterialMutationMutation in LacI S286L- Please see depositor comm…PromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD263
Plasmid#125094PurposeExpression of torR(1-134aa)-ccaR(127-234aa), ompR137 chimera under PLtetO-1, output Promoter PcpcG2-172DepositorInsertstorR(REC)-ccaR(DBD)137
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD266
Plasmid#125096PurposeExpression of torR(1-134aa)-yxdJ(132-229aa), ompR137 chimera under PLtetO-1, output Promoter PyxdJ181DepositorInsertstorR(REC)-yxdJ(DBD)137
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD281
Plasmid#125100PurposeExpression of SO_4428(1-127aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110DepositorInsertsSO_4428(REC)-psdR(DBD)137
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD285
Plasmid#125102PurposeExpression of SO_4623(1-124aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110DepositorInsertsSO_4623(REC)-psdR(DBD)137
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only