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Showing: 321 - 360 of 4109 results
  1. pJBEI-6411

    Plasmid
    #47050
    Purpose
    BglBrick plasmid (=pBbB8k-P450) coding for a cytochrome P450 and its associated reductases to oxidize limonene to perillyl alcohol in E. coli
    Depositor
    Insert
    ahpG-ahpH-ahpI coding for cytochrome P450 (CYP153A6) and its associated ferredoxin and ferredoxin reductase
    Use
    Synthetic Biology; Bglbrick
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pbad
    Available since
    Sept. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
  2. TetO-FUW-VdC9BV

    Plasmid
    #62195
    Purpose
    Expresses RNA-Guided, Nuclease-Inactive VP64:dCas9-BFP:VP64—VdC9BV—Fusion Protein to Enable Transactivation of Endogenous Genes
    Depositor
    Insert
    VP64dCas9BFPVP64
    Use
    Lentiviral
    Tags
    Two VP64s tagged to dCas9 fused to BFP
    Expression
    Mammalian
    Mutation
    D10A H840A (catalytically inactive) Cas9 (dCas9)
    Promoter
    Available since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only
  3. pRB117.2

    Plasmid
    #181949
    Purpose
    aTc-inducible expression of NarL with N-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuS
    Depositor
    Insert
    mNG-NarL; mCherry; tetR (narL, AX04_RS23485 Synthetic, narL - E. coli)
    Use
    Tags
    mNeonGreen
    Expression
    Bacterial
    Mutation
    See Depositor Comments Below
    Promoter
    mNG-NarL-PLtetO-1; mCherry-PdcuS; tetR-J23106
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
  4. pSR284

    Plasmid
    #125093
    Purpose
    Expression of ompR(1-122aa)-manR(120-234aa) chimera under PLtetO-1, output Promoter PmntCAB143
    Depositor
    Inserts
    ompR(REC)-manR(DBD)122
    sfgfp
    tetR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 26, 2019
    Availability
    Academic Institutions and Nonprofits only
  5. pSR241

    Plasmid
    #125091
    Purpose
    Expression of ompR(1-137aa)-yxdJ(132-229aa) chimera under PLtetO-1, output Promoter PyxdL181
    Depositor
    Inserts
    ompR(REC)-yxdJ(DBD)137
    sfgfp
    tetR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 26, 2019
    Availability
    Academic Institutions and Nonprofits only
  6. pKD279

    Plasmid
    #125099
    Purpose
    Expression of SO_4388(1-130aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110
    Depositor
    Inserts
    SO_4388(REC)-psdR(DBD)137
    sfgfp
    tetR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 24, 2019
    Availability
    Academic Institutions and Nonprofits only
  7. pRB113.2

    Plasmid
    #181948
    Purpose
    aTc-inducible expression of NarL with C-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuS
    Depositor
    Insert
    NarL-mNG; mCherry; tetR (AX04_RS23485, narL Synthetic, narL - E. coli)
    Use
    Tags
    mNeonGreen
    Expression
    Bacterial
    Mutation
    Promoter
    NarL-mNG-PLtetO-1; mCherry-PdcuS; tetR-J23106
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
  8. pEG03

    Plasmid
    #73841
    Purpose
    Encodes PhyB917 with a C-terminal histidine tag, for protein expression in Dicty.
    Insert
    PhyB(1-917) with C-term his tag (PIL2 Mustard Weed)
    Use
    Dictyostelium discoideum expression
    Tags
    6x his tag
    Expression
    Mutation
    Promoter
    Available since
    June 28, 2016
    Availability
    Academic Institutions and Nonprofits only
  9. pKD265

    Plasmid
    #125095
    Purpose
    Expression of torR(1-134aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110
    Depositor
    Inserts
    torR(REC)-psdR(DBD)137
    sfgfp
    tetR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 26, 2019
    Availability
    Academic Institutions and Nonprofits only
  10. pSR322

    Plasmid
    #125116
    Purpose
    Expression of uhpA(1-123aa)-ydfI(126-213aa), narL131 chimera under PLtetO-1, output Promoter PydfJ115
    Depositor
    Inserts
    uhpA(REC)-ydfI(DBD)131
    sfgfp
    tetR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 24, 2019
    Availability
    Academic Institutions and Nonprofits only
  11. pRB116.2

    Plasmid
    #181946
    Purpose
    aTc-inducible expression of TorR with C-terminal mNeonGreen fusion. Also contains mCherry under TorR-controlled promoter PtorCAD129
    Depositor
    Insert
    TorR-mNG; mCherry; tetR (AX04_RS23485, torR Synthetic, torR - E. coli)
    Use
    Tags
    mNeonGreen
    Expression
    Bacterial
    Mutation
    Promoter
    TorR-mNG-PLtetO-1; mCherry-PtorCAD129; tetR-J23106
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
  12. pRB120.2

    Plasmid
    #181947
    Purpose
    aTc-inducible expression of TorR with N-terminal mNeonGreen fusion. Also contains mCherry under TorR-controlled promoter PtorCAD129
    Depositor
    Insert
    mNG-TorR; mCherry; tetR (torR, AX04_RS23485 Synthetic, torR - E. coli)
    Use
    Tags
    mNeonGreen
    Expression
    Bacterial
    Mutation
    Promoter
    mNG-TorR-PLtetO-1; mCherry-PtorCAD129; tetR-J23106
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
  13. pRB133.2

    Plasmid
    #181950
    Purpose
    aTc-inducible expression of PhoP with C-terminal mNeonGreen fusion. Also contains mCherry under PhoP-controlled promoter PvirK
    Depositor
    Insert
    PhoP-Salmonella enterica subsp. enterica serovar Typhimurium (AX04_RS23485 Synthetic, PhoP-Salmonella enterica subsp. enterica serovar Typhimurium)
    Use
    Tags
    mNeonGreen
    Expression
    Bacterial
    Mutation
    Promoter
    PhoP-mNG-PLtetO-1; mCherry-PvirK; tetR-J23106
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
  14. pcDNA3_HLA-cmyc-GRM3 C127A TMD Y616M

    Plasmid
    #140454
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and Y616M mutant
    Depositor
    Insert
    GRM3 (GRM3 Human)
    Use
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine, tyrosine 616 to methioni…
    Promoter
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  15. pcDNA3_HLA-cmyc-GRM3 C127A TMD L624N

    Plasmid
    #140455
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and L624N mutant
    Depositor
    Insert
    GRM3 (GRM3 Human)
    Use
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine, leucine 624 to asparagine
    Promoter
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  16. pcDNA3_HLA-cmyc-GRM3 C127A TMD A634N

    Plasmid
    #140456
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and A634N mutant
    Depositor
    Insert
    GRM3 (GRM3 Human)
    Use
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine, alanine 634 to asparagine
    Promoter
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  17. pCEP4_HLA-flag-GRM3 C127A-VN

    Plasmid
    #140449
    Purpose
    Mammalian expression plasmid for FLAG-tagged mGluR3 fused to the N-terminal half of split fluorescent Venus (VN)
    Depositor
    Insert
    GRM3 (GRM3 Human)
    Use
    Tags
    FLAG tag, Signal/leader sequence from HLA class I…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine
    Promoter
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  18. pCEP4_HLA-myc-GRM3 C127A-VC

    Plasmid
    #140450
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 fused to the C-terminal half of split fluorescent Venus (VC)
    Depositor
    Insert
    GRM3 (GRM3 Human)
    Use
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine
    Promoter
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  19. pcDNA3_HLA-cmyc-GRM3 C127A TMD I578A

    Plasmid
    #140452
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and I578A mutant
    Depositor
    Insert
    GRM3 (GRM3 Human)
    Use
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine, isoluecine 578 to alanine
    Promoter
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  20. pcDNA3_HLA-cmyc-GRM3 C127A TMD F588V

    Plasmid
    #140453
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and F588V mutant
    Depositor
    Insert
    GRM3 (GRM3 Human)
    Use
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine, phenylalanine 588 to val…
    Promoter
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  21. pKD277

    Plasmid
    #125098
    Purpose
    Expression of SO_2193(1-134aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110
    Depositor
    Inserts
    SO_2193(REC)-psdR(DBD)137
    sfgfp
    tetR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
  22. pSR322.2

    Plasmid
    #125117
    Purpose
    Expression of uhpA(1-118aa)-ydfI(121-213aa), narL126 chimera under PLtetO-1, output Promoter PydfJ115
    Depositor
    Inserts
    uhpA(REC)-ydfI(DBD)126
    sfgfp
    tetR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
  23. pKD287

    Plasmid
    #125103
    Purpose
    Expression of SO_4637(1-127aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110
    Depositor
    Inserts
    SO_4637(REC)-psdR(DBD)137
    sfgfp
    tetR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 28, 2019
    Availability
    Academic Institutions and Nonprofits only
  24. pKD263

    Plasmid
    #125094
    Purpose
    Expression of torR(1-134aa)-ccaR(127-234aa), ompR137 chimera under PLtetO-1, output Promoter PcpcG2-172
    Depositor
    Inserts
    torR(REC)-ccaR(DBD)137
    sfgfp
    tetR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 26, 2019
    Availability
    Academic Institutions and Nonprofits only
  25. pKD266

    Plasmid
    #125096
    Purpose
    Expression of torR(1-134aa)-yxdJ(132-229aa), ompR137 chimera under PLtetO-1, output Promoter PyxdJ181
    Depositor
    Inserts
    torR(REC)-yxdJ(DBD)137
    sfgfp
    tetR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 26, 2019
    Availability
    Academic Institutions and Nonprofits only
  26. pKD281

    Plasmid
    #125100
    Purpose
    Expression of SO_4428(1-127aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110
    Depositor
    Inserts
    SO_4428(REC)-psdR(DBD)137
    sfgfp
    tetR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 26, 2019
    Availability
    Academic Institutions and Nonprofits only
  27. pKD285

    Plasmid
    #125102
    Purpose
    Expression of SO_4623(1-124aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110
    Depositor
    Inserts
    SO_4623(REC)-psdR(DBD)137
    sfgfp
    tetR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 26, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 321 - 360 of 4109 results