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Plasmid#193920PurposeHomology arms and mEGFP-SGLRS for N-terminus tagging of human G3BP stress granule assembly factor 1DepositorInsertG3BP1 Homology Arms with mEGFP-SGLRS (G3BP1 Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceDec. 22, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMOD_C0000
Plasmid#91081PurposeModule C, Promoter: none, Gene: none (BaeI site for GT donor cloning), Terminator: noneDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-29: LMNB1-mTagRFP-T
Plasmid#114403PurposeHomology arms and mTagRFPT-linker sequence for N-terminus tagging of human LMNB1DepositorInsertLMNB1 Homology Arms with mTagRFPT-linker (LMNB1 Human)
UseCRISPR; Donor templateTagsmTagRFPT-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceSept. 4, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
AICSDP-43: GJA1-mEGFP
Plasmid#109120PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human GJA1DepositorInsertGJA1 Homology Arms with linker-mEGFP (GJA1 Human)
UseCRISPR; Donor templateTagslinker-mEGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceMay 4, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRE57I-GNAc
Plasmid#170637PurposeDNA donor for transpositionDepositorInsertMini-Tn6677, GNAc cassette, lacI
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYZ172
Plasmid#98409PurposeDonor DNA plasmid to introduce lys9 deletion in S. pombe. Linearization with Not1 digestion.DepositorInsertsupstream homologous region to delete lys9 in genome by recombination
downstream homologous region to delete lys9 in genome by recombination
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOC2 Tubb3-GFP KI
Plasmid#183432PurposeCreOFF knock-in for beta3-tubulin-GFP (amino acid position: stop codon)DepositorInsertgRNA and GFP donor
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-27:SLC25A17-mEGFP
Plasmid#101784PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human SLC25A17DepositorInsertSLC25A17 Homology Arms with linker-mEGFP (SLC25A17 Human)
UseCRISPR; Donor templateTagslinker-mEGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceOct. 31, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTF-lacZ-rfp
Plasmid#153037PurposeCRISPR-Cas12a genome editing in E. coli. Contains a constitutively CRISPR array targeting the lacZ gene in E. coli genome and donor DNA to insert an constitutively expressed rfp reporter gene.DepositorInsertCRISPR array
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOC1 Gria1-GFP-FRB KI
Plasmid#183428PurposeCreOFF knock-in for GluA1-GFP-FRB (amino acid position: stop codon)DepositorInsertgRNA and GFP-FRB donor
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOC2 Dlg4-Halo-FKBP KI
Plasmid#183435PurposeCreOFF knock-in for PSD95-Halo-FKBP (amino acid position: R721)DepositorInsertgRNA and Halo-FKBP donor
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-41: ATP2A2-mEGFP
Plasmid#114405PurposeHomology arms and mEGFP-linker sequence for N-terminus tagging of human ATP2A2DepositorInsertATP2A2 Homology Arms with mEGFP-linker (ATP2A2 Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceSept. 4, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
AAVS1-PuroR Xlone eGFP
Plasmid#179837PurposeDoxycycline-inducible expression of eGFPDepositorInserteGFP
UseCRISPR, Synthetic Biology, and TALEN ; Donor plas…TagsExpressionMammalianMutationPromoterTRE3GSAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_His2-GFP11
Plasmid#153511PurposeHis2-GFP11 C-terminal Tag Donor PlasmidDepositorInsertHis2-GFP11
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOC3 smFP-HA-Cacna1e KI
Plasmid#183436PurposeCreON FlpOFF knock-in for smFP-HA-CaV2.3 (amino acid position: G5)DepositorInsertgRNA and smFP HA donor
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_Kma-Inulinase SP
Plasmid#153492PurposeKluyveromyces marxianus Inulinase Signal Peptide Donor PlasmidDepositorInsertInulinase SP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-22:CETN2-mTagRFP-T
Plasmid#101781PurposeHomology arms and mTagRFPT-linker sequence for N-terminus tagging of human CETN2DepositorInsertCETN2 Homology Arms with mTagRFPT-linker (CETN2 Human)
UseCRISPR; Donor templateTagsmTagRFPT-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceOct. 31, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTF-ahpC-rfp
Plasmid#153035PurposeFor CRISPR-Cas12a genome editing in E. coli. Contains a constituitively expressed CRISPR array targeting the ahpC gene in E. coli and donor DNA to insert an rfp translational fusion in the ahpC gene.DepositorInsertCRISPR array
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-83:EZH2-mEGFP
Plasmid#164499PurposeHomology arms and mEGFP-linker sequence for N-terminus tagging of human EZH2DepositorInsertEZH2 Homology Arms with mEGFP-linker (EZH2 Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceFeb. 17, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCR1075
Plasmid#111093PurposeExpresses eGFP donor in pUC19DepositorInserteGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 18, 2018AvailabilityAcademic Institutions and Nonprofits only