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Plasmid#128412PurposeVanillic acid inducible production of lysostaphin. LsyT with the amyE signaling peptide for secretion.DepositorInsertLysostaphin (epr Staphylococcus simulans)
UseSynthetic BiologyTagsamyE SPExpressionBacterialMutationPromoterPspank(V)Available sinceSept. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCpfSA
Plasmid#187890PurposeCRISPR/Cpf1-based Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureusDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNR291
Plasmid#79555PurposeOutput plasmid: contains 5-state register that regulates expression of GFP, RFP, and BFP. Expresses a unique fluorescent signature in each state.DepositorInsertsGFPmut3b
mTagBFP
mRFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
BiP-mGFP P495L
Plasmid#62232PurposeMutant of hamster BiP-mGFP unable to bind clients.DepositorInserthamster BiP-mGFP-KDEL
UseTagsmGFPExpressionMammalianMutationPromotercmvAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUdOm1.0
Plasmid#210298PurposeEmpty backbone with ampicillin resistance for high expression in mammalian cells with an added 5'UTRDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1a-F0ATPMLS-mCherry-GFP1-10
Plasmid#211466PurposeLentivirus backbone expressing mCherry and GFP1-10 fusion protein with a mitochondrial localization signalDepositorInsertmito-mCherry-GFP1-10
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYP-HaloTag
Plasmid#188907PurposeExpresses synaptic HaloTagDepositorInsertSYP (Syp Mouse)
UseLentiviralTagsHaloTagExpressionMutationPromoterAvailable sinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBTSG
Plasmid#159420PurposeTransient expression of proteins with a C-terminal thermostable GFP (TGP) tag in mammalian cellsDepositorInsertthermostable GFP (TGP)
UseTagspolyhistidine (poly-His)ExpressionMammalianMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJK240
Plasmid#71127PurposeExpression of Acetobacter aceti 1023 citrate synthase (AaCS) in E. coliDepositorInsertcitrate synthase
UseTagsGyrA intein and His6ExpressionBacterialMutationPromoterT7Available sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEpic
Plasmid#84372Purpose3rd-generation lentiviral destination vector for gateway cloning with puromycin resistance.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
punc-119cR
Plasmid#21596DepositorInsertunc-119:mCherry (unc-119 Nematode)
UseAdds unc-119 marker to amp resistant plasmidsTagsmCherryExpressionWormMutationN/APromoterAvailable sinceAug. 26, 2009AvailabilityAcademic Institutions and Nonprofits only -
PTEN-mut (negative control)
Plasmid#86646Purposeexpression of control shRNADepositorInsertPTEN (control)
UseRNAiTagsExpressionMammalianMutationPromoterH1Available sinceJune 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-c-fos-iRFP-B3-repRNA-A
Plasmid#159926PurposeTimestamp driven by a c-Fos promoter, with RNA barcode CTG, AKA 187.DepositorInsertiRFP-Timestamp A
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEN_TmiRc3_shURB5
Plasmid#81066PurposeGateway entry vector encoding shRNA to UBR5DepositorInsertshRNA targeting UBR5
UseCloningTagsExpressionMutationPromoterN/AAvailable sinceJan. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYTSG
Plasmid#159419PurposeExpression of proteins with a C-terminal thermostable GFP (TGP) tag in Saccharomyces cerevisiaeDepositorInsertthermostable GFP (TGP)
UseTagspolyhistidine (poly-His)ExpressionYeastMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEN_TmiRc3_shNT
Plasmid#81067PurposeGateway entry vector encoding non-targeting shRNADepositorInsertnon-targeting shRNA
UseCloningTagsExpressionMutationPromoterN/AAvailable sinceJan. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a.MJ0285
Plasmid#11304DepositorInsertsmall heat shock protein (MJ_0285 M. jannaschii)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 10, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEF mito-cGFP (matrix)
Plasmid#188904PurposeExpresses mitochondrial GFPDepositorInsertmitochondrial GFP
UseTagsMitochondrial leader sequence: MSVLTPLLLRGLTGSARR…ExpressionMammalianMutationPromoterAvailable sinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFTSG
Plasmid#162389PurposeExpression of proteins with a C-terminal thermostable GFP (TGP) tag in insect cellDepositorInsertthermostable GFP (TGP)
UseTagsHisExpressionInsectMutationPromoterAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT7_circRAJ31_C873U
Plasmid#69932PurposeExpresses RAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. This mutant is deficient in circularisation. Under control of a T7 promoter.DepositorInsertpT7_circRAJ31_C873U
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 10, 2016AvailabilityAcademic Institutions and Nonprofits only