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pcDNA3/Myc-DNMT1
Plasmid#36939PurposeMammalian expression of human DNMT1 with myc tagDepositorInsertDNMT1 (DNMT1 Human)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-DDB1
Plasmid#19918DepositorInsertDDB1 (Damage-specific DNA binding protein 1) (DDB1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
GSK3betaS9A (1016)
Plasmid#49492Purposeexpresses human Gsk3beta with S9A (constitutively active) mutationDepositorInsertGSK3beta S9A (GSK3B Human)
UseTagsExpressionMammalianMutationS9A (constitutively active)PromoterCMVAvailable sinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FLAG-GPD1
Plasmid#32487DepositorInsertGPD1 (Gpd1 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDN-D2irC6kwh
Plasmid#44725DepositorInsertspCMV-D2i promoter
mCherry
UseSynthetic Biology; Expression regulator/reporterTagsRabbit β-globin intron II and WPREExpressionMammalianMutationInitiator motif (Inr) displaced relative to pCMV-…PromoterpCMV-D2iAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
GFP-SP110C
Plasmid#65393PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorInsertSP110C
UseTagsEmGFP and V5ExpressionMammalianMutationnonePromoterCMVAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDN-D2irG4kwh
Plasmid#44723DepositorInsertspCMV-D2i promoter
EGFP
UseSynthetic Biology; Expression regulator/reporterTagsRabbit β-globin intron II and WPREExpressionMammalianMutationInitiator motif (Inr) displaced relative to pCMV-…PromoterpCMV-D2iAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
GFP-SP110A
Plasmid#65392PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorInsertSP110A
UseTagsEmGFP and V5ExpressionMammalianMutationnonePromoterCMVAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBT259_(pRosa26-G-tTA2)
Plasmid#36878DepositorInsertsGFP
tTA2
beta-globin intron
Neo
diphteria toxin A
UseTagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Hmga2 ATG wt
Plasmid#52034Purposeencodes wt HMGA2DepositorInsertHmga2 (Hmga2 Mouse)
UseTagsExpressionMammalianMutationstarting ATG deletedPromoterCMVAvailable sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Hmga2 ATG m7
Plasmid#52035Purposeencodes mutant HMGA2DepositorInsertHmga2 (Hmga2 Mouse)
UseTagsExpressionMammalianMutationall seven let-7 sites in the 3′ UTR mutated (‘m7’)PromoterCMVAvailable sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EngPDm
Plasmid#64040PurposeMammalian expression of mutant paired domain of Pax8 fused to the N-terminal repressor domain of the drosophila engrailed protein (Eng)DepositorInsertPax8 paired domain (Pax8 Mouse)
UseTagsEng (N-terminal repressor domain of the drosophi…ExpressionMammalianMutationpaired domain, aa 1-147, with aa 30 to 32 changed…PromoterCMVAvailable sinceApril 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EngHDm
Plasmid#64038PurposeMammalian expression of TTF-1 mutant homeodomain fused to the N-terminal repressor domain of the drosophila engrailed protein (Eng)DepositorInsertTTF-1 (TTF1 dog)
UseTagsEng (N-terminal repressor domain of the drosophi…ExpressionMammalianMutationhomeodomain, aa 154-226, with aa 48–50 replaced b…PromoterCMVAvailable sinceApril 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Hmga2 shMut wt
Plasmid#52036Purposeencodes shMut HMGA2DepositorInsertHmga2 (Hmga2 Mouse)
UseTagsExpressionMammalianMutationshRNA binding site mutatedPromoterCMVAvailable sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
p2xSTAT6-Luc2P
Plasmid#35486DepositorInsertSTAT6 promoter
UseLuciferaseTagsExpressionMammalianMutationPromoter2x Stat6-c/EBPAvailable sinceMarch 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDEST26-MSRA-C-HA
Plasmid#195177PurposeVector for transient expression of MSRA gene with C-terminal HA tag, gene flanked by att sites for gateway cloningDepositorInsertMSRA (MSRA Human)
UseTagsHAExpressionMammalianMutationPromoterCMV promoterAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CRISPR-Puro
Plasmid#208646PurposepcDNA3.1 based empty vector for transient transfection of sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 (Addgene#52961), lenti virus sequences not included.DepositorTypeEmpty backboneUseCRISPRTagsClawed frog nucleoplasmin NLS, Flag, Puromycin re…ExpressionMammalianMutationPromoterCMV and U6 in tandemAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST26-PPP2CB-C-HA
Plasmid#195179PurposeVector for transient expression of PPP2CB gene with C-terminal HA tag, gene flanked by att sites for gateway cloningDepositorInsertPPP2CB (PPP2CB Human)
UseTagsHAExpressionMammalianMutationPromoterCMV promoterAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
UVR8-mCherry
Plasmid#49804Purposeexpresses mCherry fused to UVR8 under the CMV promoterDepositorInsertUVR8-mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2013AvailabilityAcademic Institutions and Nonprofits only
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