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pAAV-CAG-FLuc

Plasmid

#83281

Purpose

recombinant AAV vector packaging single-stranded Firefly Luciferase under the CAG promoter (includes WPRE element)

Depositor

Mark Kay

Article

Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

Insert

Firefly Luciferase

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLuc

Plasmid

#83281

Purpose

recombinant AAV vector packaging single-stranded Firefly Luciferase under the CAG promoter (includes WPRE element)

Depositor

Mark Kay

Article

Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

Insert

Firefly Luciferase

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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CAG-BFP-sesRNA-GFP-WPRE-pA

Plasmid

#192063

Purpose

Expression of BFP, sesRNA in mammalian cells, with GFP as efRNA.

Depositor

Josh Huang

Article

Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

Insert

sesRNA for tdTom

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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CAG-BFP-sesRNA-GFP-WPRE-pA

Plasmid

#192063

Purpose

Expression of BFP, sesRNA in mammalian cells, with GFP as efRNA.

Depositor

Josh Huang

Article

Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

Insert

sesRNA for tdTom

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAAVS1-TRE-Cas9- puro-polyA-CAG-rtTA

Plasmid

#107270

Purpose

Expresses Cas9 upon induction with doxicycline.

Depositor

Pablo Menéndez

Article

Castano et al Stem Cell Res. 2017 May;21:137-140. doi:

Inserts

hSpCas9
rtTA
bGH polyA-SV40polyA

Tags

3xFlag, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Promoter

CAG and TRE

Available Since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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pAAVS1-TRE-Cas9- puro-polyA-CAG-rtTA

Plasmid

#107270

Purpose

Expresses Cas9 upon induction with doxicycline.

Depositor

Pablo Menéndez

Article

Castano et al Stem Cell Res. 2017 May;21:137-140. doi:

Inserts

hSpCas9
rtTA
bGH polyA-SV40polyA

Tags

3xFlag, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Promoter

CAG and TRE

Available Since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG dCas9-KRAB-2A-EGFP

Plasmid

#92396

Purpose

CAG-driven ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. Contains 2A-EGFP reporter. For targeted enhancer inactivation in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

dCas9_KRAB_2A_EGFP

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG dCas9-KRAB-2A-EGFP

Plasmid

#92396

Purpose

CAG-driven ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. Contains 2A-EGFP reporter. For targeted enhancer inactivation in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

dCas9_KRAB_2A_EGFP

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pSLQ2818 pPB: CAG-PYL1-KRAB-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9

Plasmid

#84241

Purpose

Expresses ABA-inducible KRAB-Sp dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

ABI-tagBFP-Sp dCas9
PYL1-KRAB

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), ABI, HA tag, IRES-Puro, PYL1, and t…

Expression

Mammalian

Promoter

CAG and PGK

Available Since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pSLQ2818 pPB: CAG-PYL1-KRAB-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9

Plasmid

#84241

Purpose

Expresses ABA-inducible KRAB-Sp dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

ABI-tagBFP-Sp dCas9
PYL1-KRAB

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), ABI, HA tag, IRES-Puro, PYL1, and t…

Expression

Mammalian

Promoter

CAG and PGK

Available Since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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AAV pCAG-FSF-FLEX-EGFP-WPRE-bGHpA

Plasmid

#65455

Purpose

Can be used to generate AAV virus that will express EGFP from the CAG promoter under intersectional control by Flp and Cre recombinases

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

EGFP

Use

AAV

Promoter

CAG

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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AAV pCAG-FSF-FLEX-EGFP-WPRE-bGHpA

Plasmid

#65455

Purpose

Can be used to generate AAV virus that will express EGFP from the CAG promoter under intersectional control by Flp and Cre recombinases

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

EGFP

Use

AAV

Promoter

CAG

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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paavCAG-JxON-pre-mGRASP-mCerulean

Plasmid

#51903

Purpose

JxOn-pre

Depositor

Jinhyun Kim

Article

Druckmann et al Neuron. 2014 Feb 5;81(3):629-40. doi: 10

Insert

presynaptic mGRASP

Use

AAV and Cre/Lox

Promoter

CAG

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only

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paavCAG-JxON-pre-mGRASP-mCerulean

Plasmid

#51903

Purpose

JxOn-pre

Depositor

Jinhyun Kim

Article

Druckmann et al Neuron. 2014 Feb 5;81(3):629-40. doi: 10

Insert

presynaptic mGRASP

Use

AAV and Cre/Lox

Promoter

CAG

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pCXLE-hGLIS1

Plasmid

#37625

Purpose

Non-integrating (episomal) expression of human GLIS1

Depositor

Shinya Yamanaka

Article

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Insert

human GLIS1 (GLIS1 Human)

Expression

Mammalian

Mutation

T110A, A187G, I221T

Promoter

CAG

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pCXLE-hGLIS1

Plasmid

#37625

Purpose

Non-integrating (episomal) expression of human GLIS1

Depositor

Shinya Yamanaka

Article

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Insert

human GLIS1 (GLIS1 Human)

Expression

Mammalian

Mutation

T110A, A187G, I221T

Promoter

CAG

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pMXs-IP-EGFP-hFIP200

Plasmid

#38192

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

FIP200 (RB1CC1 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 27, 2012

Availability

Academic Institutions and Nonprofits only
pBRPB CAG-mCherry-IP

Plasmid

#106333

Purpose

PiggyBac transposon, mCherry-IRES-puro labeling under the control of CAG promoter. Used in chimera experiment of ES cells.

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Insert

mCherry-IRES-Puro

Use

Piggybac transposon

Expression

Mammalian

Promoter

CAG

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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pBRPB CAG-mCherry-IP

Plasmid

#106333

Purpose

PiggyBac transposon, mCherry-IRES-puro labeling under the control of CAG promoter. Used in chimera experiment of ES cells.

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Insert

mCherry-IRES-Puro

Use

Piggybac transposon

Expression

Mammalian

Promoter

CAG

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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pAAVS1-P-CAG-GFPluc2

Plasmid

#80493

Purpose

donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP-luciferase fusion protein expression

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Insert

GFPluc2

Use

Donor vector (human)

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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