We narrowed to 3,264 results for: actin

1. pORANGE_NFL linker-3xFLAG KI

   Plasmid

   #182679

   Purpose

   Expression of spCas9, gRNA targeting the end of mouse Nefl gene and donor linker-3xFLAG. Can be used for C-terminal tagging of endogenous NFL with a linker-3xFLAG tag.

   Depositor

   Ivana Nikić-Spiegel

   Article

   Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

   Insert

   Nefl-targeting gRNA and linker-3xFLAG donor sequence (Nefl Mouse)
   

   Expression

   Mammalian

   Promoter

   U6 and chicken beta-actin promoter

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. miniSOG-VASP-5

   Plasmid

   #57778

   Purpose

   Localization: Actin/Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

   Depositor

   Michael Davidson

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Insert

   VASP
   

   Tags

   miniSOG

   Expression

   Mammalian

   Mutation

   A209T in VASP

   Promoter

   CMV

   Available Since

   Jan. 29, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pCAG-TetON-3G

   Plasmid

   #96963

   Purpose

   Mammalian expression of Clontech rtTA driven by human beta-actin promoter

   Depositor

   Elena Cattaneo

   Article

   Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Aug. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGs-NLS-TIR1_P2A_NES-TIR1

   Plasmid

   #117699

   Purpose

   Expression of NLS-(Os)TIR1-HA and Myc-NES-(Os)TIR1from one mRNA

   Depositor

   Joerg Mansfeld

   Article

   Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

   Inserts

   TRANSPORT INHIBITOR RESPONSE 1
   TRANSPORT INHIBITOR RESPONSE 1
   

   Tags

   HA tag, Myc-NES, and NLS

   Expression

   Mammalian

   Mutation

   codon optimized for murine expression

   Promoter

   None, 2A peptide from porcine teschovirus-1 polyp…

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-EB3mCherry_cmv-LifeActGFP

   Plasmid

   #133927

   Purpose

   Live dual labeling of the actin network and polymerizing microtubules for dynamic analysis of the cytoskeleton

   Depositor

   Sebastián Dupraz, Frank Bradke

   Article

   Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

   Insert

   EB3-mCherry and Lifeact-GFP (MAPRE3 Human)
   

   Expression

   Mammalian

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV5 BRGI-Flag

   Plasmid

   #19143

   Depositor

   Joan Massague

   Article

   Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

   Insert

   SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Sept. 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. FLAG-mBAF45c IRES GFP

   Plasmid

   #17882

   Depositor

   Jerry Crabtree

   Article

   Lessard et al Neuron. 2007 Jul 19. 55(2):201-15.

   Insert

   BAF45c (Dpf3 Mouse)
   

   Tags

   FLAG and IRES EGFP

   Expression

   Mammalian

   Available Since

   May 13, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pCIG-V5-Snail2-IRES-nls-GFP

   Plasmid

   #44282

   Depositor

   Martin Cheung

   Article

   Liu et al Proc Natl Acad Sci U S A. 2013 Feb 19;11

   Insert

   V5-Snail2 (SNAI2 Chicken)
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV-IE-enhancer and chicken beta-actin promoter

   Available Since

   Aug. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCardinal-Lifeact-7

   Plasmid

   #54663

   Purpose

   Localization: Actin, Excitation: 604, Emission: 659

   Depositor

   Michael Davidson

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Type

   Empty backbone

   Tags

   mCardinal-Lifeact

   Expression

   Mammalian

   Available Since

   June 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. LifeAct-mGreenLantern

    Plasmid

    #164459

    Purpose

    Localization of mGreenLantern fluorescent protein to actin

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Tags

    LifeAct

    Expression

    Mammalian

    Mutation

    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

    Promoter

    CMV

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pExpreS2-1-GFP

    Plasmid

    #175363

    Purpose

    ZeoR overexpression of cytosolic GFP in Drosophila ExpreS2ion cells

    Depositor

    ExpreS2ion Biotechnologies

    Article

    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

    Insert

    sgGFP
    

    Expression

    Insect

    Mutation

    F64L, S65C, and I167T

    Promoter

    Fused Actin-HSP70

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAX-EGFP-FBP17

    Plasmid

    #178365

    Purpose

    Expresses FBP17 c-terminal labeled with EGFP in mammalian cells

    Depositor

    Erik Dent

    Article

    Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

    Insert

    Formin Binding Protein 17 (FNBP1 Human)
    

    Expression

    Mammalian

    Promoter

    Chicken Beta Actin

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pORANGE GFP-Actb KI #2

    Plasmid

    #139666

    Purpose

    Endogenous tagging of β-actin: N-terminal (amino acid position: before startcodon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. 2xFKBP-mCh-KDEL

    Plasmid

    #173010

    Purpose

    FKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of mCherry. Contains IRES following ORF for cloning cargo.

    Depositor

    Matthew Kennedy

    Article

    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    2xFKBP-mCh-KDEL
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    Chicken beta actin (CAG)

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. ITPKA-mTurquoise2

    Plasmid

    #137811

    Purpose

    In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    ITPKA (ITPKA Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CAG-EGFP-P2A-FusionRed

    Plasmid

    #191108

    Purpose

    Mammalian cell line expression plasmids for production of EGFP and FusionRed proteins

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    pAAV-CAG-EGFP-P2A-FusionRed
    

    Use

    AAV

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    chicken β-actin promoter

    Available Since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBa.GFP-KIF1Bbeta tail 387-1770

    Plasmid

    #134620

    Purpose

    Mammalian expression of mouse KIF1Bbeta 387-1170

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF1Bbeta (Kif1b Mouse)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 386 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available Since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTone-gata2aECE-Kaede

    Plasmid

    #132976

    Purpose

    gata2a endothelial enhancer (x6) and basal promoter driving kaede in pTol1 backbone

    Depositor

    Nathan Lawson

    Article

    Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

    Inserts

    Kaede
    gata2a endothelial enhancer (x6) and a carp b-actin basal promoter
    

    Use

    Unspecified; Transposon-mediated

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBT268_(pCA-ATG-intron-tTA2-iiTRE-tdT3Mycii)

    Plasmid

    #36880

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    tTA2
    tdT-3Myc
    insulator
    

    Tags

    3 Myc tags

    Expression

    Mammalian

    Mutation

    insertion of a beta-globin intron with a loxP sit…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available Since

    Aug. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. DHFR-mNeon-GluA1

    Plasmid

    #173009

    Purpose

    For light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Intracellular trafficking can be better visualized with mNeon.

    Depositor

    Matthew Kennedy

    Article

    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    DHFR-mNeon-GluA1
    

    Tags

    DHFR and mNeon

    Expression

    Mammalian

    Promoter

    Chicken beta actin (CAG)

    Available Since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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