1. p416 103Q GAL

   Plasmid

   #1186

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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These results may also be relevant.

1. pIMVAd39T

   Plasmid

   #98303

   Purpose

   Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or GAL2 promoter; integrated into PDC5 locus; disrupting PDC5.

   Depositor

   Claudia Vickers

   Article

   Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

   Insert

   pdc5(-277,-32)-PGAL2>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDECKO_mCherry_non-targeting

   Plasmid

   #140109

   Purpose

   CRISPR-Cas9 library validation (negative control)

   Depositor

   Roderic Guigo

   Article

   Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   gRNA1 under U6 and gRNA2 under H1

   Available since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ELF3_pet28a

   Plasmid

   #131658

   Purpose

   Bacterial expression of ETS gene ELF3

   Depositor

   Peter Hollenhorst

   Article

   Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

   Insert

   ELF3 (ELF3 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUDP012

   Plasmid

   #101167

   Purpose

   E. coli/S. cerevisiae shuttle vector carrying amdS andSpcas9D147Y P411T and expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 and in S. pastorianus (HH-gRNASeILV6-HDV)

   Depositor

   Jean-Marc Daran

   Article

   de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

   Insert

   HH-gRNA-HDV targetting SeILV6 in S. pastorianus
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ScTDH3

   Available since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. DNMT3L-dCas9

   Plasmid

   #126587

   Purpose

   DNMT3L fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

   Depositor

   David Segal

   Article

   O'Geen et al Epigenetics Chromatin. 2019 May 3;12(1):

   Insert

   DNMT3L (DNMT3L Human)
   

   Use

   CRISPR

   Tags

   3XFLag-NLS-DNMT3L-dCas9-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pICSL11055

   Plasmid

   #68252

   Purpose

   Level 1 Golden Gate Cassette: Plant kanamycin resistance cassette

   Depositor

   Nicola Patron

   Article

   Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

   Insert

   Promoter:2x35s+5'UTR:Omega+CDS:nptII+3'UTR/terminator:Nos
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDGB3 alpha2 P35S:dCas9:SRDX:Tnos (GB1188)

   Plasmid

   #160608

   Purpose

   TU for the expression of the dCas9 with the repressor domain SRDX as a CT fusion (CRISPR tools)

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

   Insert

   P35s:dCas9:SRDX:tNOS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   P35S

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEM-HE-2xLyn-ERex-Venus-bPAC(F198Y)

   Plasmid

   #165489

   Purpose

   Xenopus oocyte expression of PACmn, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity

   Depositor

   Thomas Oertner

   Article

   Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

   Insert

   2xLyn-ERex-Venus-bPAC(F198Y)
   

   Use

   Cdna expression, xenopus oocyte

   Tags

   Venus

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-Flag-dSpCas9-HF1

   Plasmid

   #92115

   Purpose

   Expression plasmid for human codon-optimized dead/inactive high-fidelity SpCas9-HF1 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive SpCas9-HF1 with FLAG tag
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   D10A, N497A, R661A, Q695A, H840A, Q926A

   Promoter

   Cbh

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Direct-seq_lentiGuide-Puro-tRNA-Tail-8A8G

    Plasmid

    #157984

    Purpose

    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

    Depositor

    Lijia Ma

    Article

    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

    Insert

    S. pyogenes sgRNA cassette (tRNA-Tail-8A8G)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Direct-seq_lentiGuide-Puro-tRNA-Tetra Loop-8A8G

    Plasmid

    #157985

    Purpose

    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

    Depositor

    Lijia Ma

    Article

    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

    Insert

    S. pyogenes sgRNA cassette (tRNA-Tetra-8A8G)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET-deSpCas9-VP64-6xHis

    Plasmid

    #92117

    Purpose

    Expression of dead/inactive increased fidelity eSpCas9 (1.1)-VP64-6xHis in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive eSpCas9-NLS-3xFLAG-VP64
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A, H840A, K848A, K1003A, R1060A

    Promoter

    T7

    Available since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTE4254

    Plasmid

    #89050

    Purpose

    Expresses St chimeric gRNA and human codon-optimized StCas9

    Depositor

    Ervin Welker

    Article

    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

    Inserts

    St chimeric gRNA
    hStCas9
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and human U6

    Available since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCXLE-hK + E2-Crimson

    Plasmid

    #74946

    Purpose

    Dosage control and tracing of reprogramming episomes

    Depositor

    Ann Zovein

    Article

    Schmitt et al Stem Cell Res Ther. 2017 Jun 5;8(1):132.

    Insert

    Klf4 (KLF4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

15. pLPCX-Cx43-FS-IRES-GFP

    Plasmid

    #65438

    Purpose

    Mammalian expression of frameshift mutated Cx43 and EGFP separately driven by the ECMV IRES. For transfection or retroviral production

    Depositor

    Trond Aasen

    Article

    Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

    Insert

    Cx43 (GJA1 Human)
    

    Use

    Retroviral

    Tags

    IRES-GFP

    Expression

    Mammalian

    Mutation

    frameshift (FS) mutation 35delA

    Promoter

    CMV

    Available since

    June 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. ERG_pet28a

    Plasmid

    #131642

    Purpose

    Bacterial expression of ETS gene ERG

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    ERG (ERG Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EC71167

    Plasmid

    #154066

    Purpose

    Level 1 Position 3 Golden Gate vector, pL1M-R3-UbqP-Loxp-ER-Targ-mCherry-HDEL-Loxp-eGFP

    Depositor

    Cristobal Uauy

    Article

    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

    Insert

    ZmUBI::lox-mCHERRY-lox-T35S-eGFP-TAct
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    All BsaI, Esp3I and BPiI restriction sites were r…

    Promoter

    Zea mays Ubiquitin Promoter

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ELK3_pet28a

    Plasmid

    #131651

    Purpose

    Bacterial expression of ETS gene ELK3

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    ELK3 (ELK3 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDECKO_mCherry_CEACAM1

    Plasmid

    #140102

    Purpose

    CRISPR-Cas9 library validation

    Depositor

    Roderic Guigo

    Article

    Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    gRNA1 under U6 and gRNA2 under H1

    Available since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKS7107

    Plasmid

    #89051

    Purpose

    Expresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9

    Depositor

    Ervin Welker

    Article

    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

    Inserts

    Nm crRNA
    Nm tracrRNA
    hNmCas9
    

    Use

    CRISPR

    Tags

    HA tag, NLS, and SV40 NLS

    Expression

    Mutation

    Promoter

    EF1a and human U6

    Available since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge