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Plasmid#73131PurposeExpression in Mammalian Cells, Cas9 Nuclease to analize translocationsDepositorInsertspCas9
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
OCT4 GFP puro Donor 3
Plasmid#22211DepositorInsertSA-OCT-GFP-2APuro-PA (POU5F1 Human)
UseTagsExpressionMutationPromoterAvailable sinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBABE.puro/TO/Flag/tGFP-HuR.wt
Plasmid#110349PurposeDoxycycline inducible mammalian retroviral expression of HuR (wild type) with FLAG and turboGFP tagsDepositorInsertELAV like RNA binding protein 1 (ELAVL1 Human)
UseRetroviralTagsFLAG and tGFPExpressionMammalianMutationPromotergagAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-Smad2
Plasmid#89827PurposeKnockdown human Smad2 expression in human mammalian cellsDepositorInsertSMAD2 shRNA (SMAD2 Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF2α P531A-pBabe-puro
Plasmid#26091DepositorInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationcDNA with point mutation P531APromoterAvailable sinceSept. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL Y98H-pBabe-puro
Plasmid#19235DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationchanged Tyrosin 98 to HistidinPromoterAvailable sinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLenti-puro/RAF1-P261A
Plasmid#131728PurposeMammalian Expression of RAF1DepositorInsertRAF1 (RAF1 Human)
UseLentiviralTagsExpressionMammalianMutationP261APromoterCMVAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Puro-mGFP-WDR34
Plasmid#64251PurposeExpresses WDR34 as a fusion with monomeric GFPDepositorInsertWDR34 (WDR34 Human)
UseLentiviralTagsmGFPExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Puro-RalA-V23
Plasmid#15252DepositorInsertv-ral simian leukemia viral oncogene homolog A (RALA Human)
UseRetroviralTagsExpressionMammalianMutationchanged 23rd amino acid to valinePromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-shNup133-1
Plasmid#87334PurposeTo express shRNA against human Nup133DepositorInsertshRNA against human Nup133
UseRNAiTagsExpressionMammalianMutationPromoterH1Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTet-O-APOE-E4-T2A-Puro
Plasmid#190813PurposeExpress APOE E4 allele from tet-inducible promoter, co-expressing Puro resistance geneDepositorInsertAPOE (APOE Human)
UseLentiviralTagsExpressionMutationE4PromoterTet-OAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTet-O-APOE-E3-T2A-Puro
Plasmid#190812PurposeExpress APOE E3 allele from tet-inducible promoter, co-expressing Puro resistance geneDepositorInsertAPOE (APOE Human)
UseLentiviralTagsExpressionMutationE3PromoterTet-OAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTet-O-APOE-E2-T2A-Puro
Plasmid#190811PurposeExpress APOE E2 allele from tet-inducible promoter, co-expressing Puro resistance geneDepositorInsertAPOE (APOE Human)
UseLentiviralTagsExpressionMutationE2PromoterTet-OAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL 1-115-pBabe-puro
Plasmid#19243DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationdeleted amino acids 116 - 213 (end)PromoterAvailable sinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLV x EF1a_EKAREN4(TA)-ires-puro
Plasmid#167830PurposeControl sensor for EKAREN4DepositorInsertEKAREN4(TA)
UseLentiviralTagsnls localization motifExpressionMammalianMutationK424P, K426W, T420APromoterEF1aAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBabe JunD-Venus puro
Plasmid#58496Purposemammalian expression of Venus-tagged mouse JunDDepositorInsertJunD (Jund Mouse)
UseTagsVenusExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
attB‐mCh‐Poly(A)-PuroR
Plasmid#65529PurposePuroR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.DepositorInsertattB-mCherry
UseMouse Targeting; Bxb1TagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDH-puro/Flag PD-L1 N35Q
Plasmid#121449Purposeexpression of human PD-L1 mutant in mammalian cellsDepositorInsertCD274 (CD274 Human)
UseLentiviralTagsFlagExpressionMammalianMutationN35QPromoterCMVAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(hPLK1)-PGKpuro2ABFP-W
Plasmid#163138PurposeLentiviral gRNA plasmid targeting human PLK1 gene, co-expression of BFP tagDepositorInsertPLK1 (PLK1 Human)
UseCRISPR and LentiviralTagsBFPExpressionMammalianMutationPromoterU6Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
miniTol2 x EF1a_EKAREN4 + PGK-puro
Plasmid#167824PurposeOptimized EKAREV FRET biosensor sensor for ERKDepositorInsertEKAREN4
UseTagsnls localization motifExpressionMammalianMutationK424P, K426WPromoterEF1aAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only