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Plasmid#106321PurposeMammalian expression of mutant osteopontinDepositorInsertOPNc (SPP1 Human)
UseTagsExpressionMammalianMutationOPNc mutated splice site EEKQ-->AAAAPromoterAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR3.1-OPNc M5
Plasmid#106325PurposeMammalian expression of mutant osteopontinDepositorInsertOPNc (SPP1 Human)
UseTagsExpressionMammalianMutationOPNc splice site insertion mutant EEKQNA-->EEK…PromoterAvailable sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR3.1-OPNc M4
Plasmid#106324PurposeMammalian expression of mutant osteopontinDepositorInsertOPNc (SPP1 Human)
UseTagsExpressionMammalianMutationOPNc splice site deletion mutant EEKQNA-->EEKNAPromoterAvailable sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR3.1-OPNc M3
Plasmid#106323PurposeMammalian expression of mutant osteopontinDepositorInsertOPNc (SPP1 Human)
UseTagsExpressionMammalianMutationOPNc mutated splice site EEKQNAV-->AAAAAAAPromoterAvailable sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-HSE1-iGFPx6
Plasmid#105259PurposePlasmid for pop-in/pop-out replacement of Hse1 with Hse1-iGFPx6.DepositorInsertHse1 portion (HSE1 Budding Yeast)
UseTagsiGFPx6ExpressionYeastMutationPromoterAvailable sinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-MUP1-iYFP
Plasmid#105266PurposePlasmid for pop-in/pop-out replacement of Mup1 with Mup1-iYFP.DepositorInsertMup1 portion (MUP1 Budding Yeast)
UseTagsiYFPExpressionYeastMutationPromoterAvailable sinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
BS.YxiN.Twin1.a1-368.E152Q
Plasmid#17517DepositorInsertB. subtilis DEAD-box helicase YxiN first two domains
UseTagsintein-chitin binding domainExpressionBacterialMutationdeleted amino acid residues 369-479; E152Q mutat…PromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
MERTK_HUMAN_D0
Plasmid#79705PurposeThis plasmid encodes the kinase domain of MERTK. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertMERTK (MERTK Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
KCC2G_HUMAN_D0
Plasmid#79711PurposeThis plasmid encodes the kinase domain of KCC2G. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertKCC2G (CAMK2G Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGST-HsSyx-375-792
Plasmid#179292PurposeE. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 375-792) with N-terminal His6 + GST + TEV protease cleavage siteDepositorInsertSyx
UseTagsHis6 + GST + TEV protease cleavage siteExpressionBacterialMutationresidues 375-792 from accession number NP_0010361…PromoterT7Available sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
MARK3_HUMAN_D0
Plasmid#79740PurposeThis plasmid encodes the kinase domain of MARK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertMARK3 (MARK3 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
ABL1_HUMAN_D0
Plasmid#79727PurposeThis plasmid encodes the kinase domain of ABL1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertABL1 (ABL1 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
DYR1A_HUMAN_D0
Plasmid#79690PurposeThis plasmid encodes the kinase domain of DYR1A. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertDYRK1A (DYRK1A Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET His6 MBP TEV HDAC8_374
Plasmid#122174PurposeExpresses a truncated construct of histone deacetylase 8 for crystallography bearing an N-terminal tobacco etch virus protease-cleavable hexahistidine-maltose binding protein tagDepositorInsertHis6 MBP TEV HDAC8_374 (HDAC8 Human)
UseTagshexahistidine tag and tobacco etch virus protease…ExpressionBacterialMutationResidues Met1–Pro7 and H375-V377 have been delete…PromoterT7Available sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 6A NLRP1 UPA-CARD-FLAG E1461R
Plasmid#163990PurposeMammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tagDepositorInsertNLRP1 (NLRP1 Human)
UseTagsFLAGExpressionMammalianMutationE1461RPromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
EPHB1_HUMAN_D0
Plasmid#79694PurposeThis plasmid encodes the kinase domain of EPHB1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertEPHB1 (EPHB1 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
GAK_HUMAN_D0
Plasmid#79682PurposeThis plasmid encodes the kinase domain of GAK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertGAK (GAK Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
AURKA_HUMAN_D0
Plasmid#79739PurposeThis plasmid encodes the kinase domain of AURKA. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertAURKA (AURKA Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
KC1D_HUMAN_D0
Plasmid#79723PurposeThis plasmid encodes the kinase domain of KC1D. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertKC1D (CSNK1D Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
FGFR3_HUMAN_D0
Plasmid#79731PurposeThis plasmid encodes the kinase domain of FGFR3. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertFGFR3 (FGFR3 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only