-
Plasmid#200637PurposeRecombination plasmid for Matreyek Bxb1(GT) landing pad in dual landing pad cellsDepositorInsertVif
UseTagsExpressionMammalianMutationT27stopPromoterAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pH6-2xmNeonC
Plasmid#178463PurposeA tandem mNeonGreen fluorescent protein (2xmNeon) with an N-terminal 6xHis-tag and C-terminal cysteine in pET28a.DepositorInsertmNeonGreen dimer
UseTags6xHisExpressionBacterialMutationFree cysteine at the C-terminus of the dimerPromoterT7Available sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCZGY2727
Plasmid#193330PurposeSite specific insertion using CRISPR/Cas9 editing of C. elegans ChrIDepositorInsertHygromycin resistance
UseTagsExpressionWormMutationPromoterPrps-0Available sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCZGY2748
Plasmid#193331PurposeSite specific CRISPR/Cas9 editing of C. elegans Chr IDepositorInsertsgRNA for ttTi4348
UseTagsNoneExpressionWormMutationPromoterU6Available sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG CAG CCL20-pre-mGRASP
Plasmid#173114PurposeExpresses CCL20 sender construct in mammalian cellsDepositorInsertCCL20-pre-mGRASP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 CMV myc-Arrestin-TEVp
Plasmid#173115PurposeExpresses arrestin receiver construct in mammalian cellsDepositorInsertmyc-Arrestin-TEVp
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
p-preSufI(IAC)
Plasmid#168516PurposeModified form of pre-SufI in pET25b. With C-terminal HHHHHHC (6xHisC) extension and mutations C17I and C295A.DepositorInsertpre-SufI(IAC)
UseTags6xHisC tag and HSV tagExpressionBacterialMutationC17I and C295APromoterT7Available sinceSept. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
p8130 MSCV GFP Puro C-FlagHA
Plasmid#184521PurposeExpression of GFPDepositorInsertGFP
UseRetroviralTagsFlag, HAExpressionMutationPromoterMSCV LTRAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
NK85
Plasmid#176611PurposeTo minimize dimerization between FcRg transmembrane domains, as this cysteine forms a disulfide bridge between y chains. To manipulate ligand spacing.DepositorInsertSNAP-FCERG-mGFP
UseLentiviralTagsExpressionMammalianMutationC25APromoterAvailable sinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
NK83
Plasmid#176610PurposeTo measure Syk intensity in response to ligand clustering using DNA origamiDepositorInsertNeonGreen-tSH2 Syk (Syk Mouse, Synthetic)
UseLentiviralTagsExpressionMammalianMutationn/aPromoterAvailable sinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIB1884-ximD-PCPT
Plasmid#169109PurposeProduction of marmesin in Streptomyces xiamenensisDepositorInsertXimD and PcPT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVP2del50
Plasmid#125185PurposeExpresses the VP2del50 truncation mutant protein derived from AAV2 VP2DepositorInserthexahistidine tag
UseTagsExpressionMammalianMutationInserted start codon and hexahistidine tag at 2697PromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVP2del30
Plasmid#125184PurposeExpresses the VP2del30 truncation mutant protein derived from AAV2 VP2DepositorInserthexahistidine tag
UseTagsExpressionMammalianMutationInserted start codon and hexahistidine tag at 2637PromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pALPS puro miR30-TAK1
Plasmid#117162PurposeTarget site AGCGCCCTTCAATGGAGGAAATDepositorInsertshRNA targeting miR30
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pClodANL-FGF2NBAA
Plasmid#107873PurposeBacterial expression of FGF2 minimum export mutant-NanoLucDepositorInsertFGF2-minimal export
UseLuciferaseTagsNanoLucExpressionBacterialMutationC77A, C95A, K128Q, R129Q, K134QPromoterpro DAvailable sinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pALPS puro miR30-IRF5
Plasmid#117157PurposeTarget site TATTTCCCTGTCTCCTTGGCCDepositorInsertshRNA targeting miR30
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pALPS puro miR30-IRF7
Plasmid#117158PurposeTarget site ATAAGGAAGCACTCGATGTCGDepositorInsertshRNA targeting miR30
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pALPS puro miR30-STAT2
Plasmid#117161PurposeTarget site TTTAAGTTCCACAGACTTGGADepositorInsertshRNA targeting miR30
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCF738
Plasmid#122032PurposeEF1a-WNV-protease-GFPDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBP-BBa_J23100
Plasmid#74097PurposeBBa_J23100 promoter (iGEM collection)DepositorInsertBba_J23100 constitutive promoter (Sigma70)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterNoneAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only