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Plasmid#33242DepositorInsertATXN1L (ATXN1L Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-TWIN-STREP-FLAG-NPRL2
Plasmid#164985PurposeNPRL2 expressing vector for mammalian cellsDepositorInsertNPRL2 (NPRL2 Human)
UseTags2xSTREP FLAGExpressionMammalianMutationPromoterCAGAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA_DiCre_60.F2
Plasmid#60208PurposeExpresses a fusion protein of FRB-linker peptide 2- Cre(60-343)DepositorInsertCre recombinase (CD207 Human, Synthetic, Bacteriophage P1)
UseTagsFRAP and Nuclear localisation signalExpressionMammalianMutationamino acids 2021-2112 of FRAP with a T2098L mutat…PromoterCMVAvailable sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Cre stuffer v4
Plasmid#158032Purposelenti-viral construct with Cre recombinase and U6 driven sgRNA casette with modified tracrRNA (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMXs-hs-NRAS
Plasmid#52728Purposeretroviral expression of human NRASDepositorInsertNRAS (NRAS Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-Tet-Puro-shIAPEz_2
Plasmid#185017PurposeshRNA mediated knockdownDepositorInsertERV_IAPEz
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-Tet-Puro-shIAPEz_1
Plasmid#185016PurposeshRNA mediated knockdownDepositorInsertERV_IAPEz
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-Ef1a-mCh-Puro-GFPi
Plasmid#31848DepositorInsertGFP RNAi
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterU6 for shRNA; Ef1alpha for mCherry-puromycinAvailable sinceJan. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
DJ-1 TEV site pET15b
Plasmid#60687PurposeRecombinant protein expression of Park7DepositorInsertDJ-1 (PARK7 Human)
UseTags6xHisExpressionBacterialMutationTEV site was inserted (see comments below).PromoterT7Available sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEY61
Plasmid#191058Purposeacr-2(3.7k) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)DepositorInsertacr-2(3.7k) (acr-2 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
NET5L pEGFP-N2 (1155)
Plasmid#62035Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertNET5L (TMEM201 Human)
UseTagsEGFPExpressionMammalianMutationlong formPromoterCMVAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pNAS1b split mCherry NEDD4-2 WW2
Plasmid#111883PurposeMode #2 phosphosite expression (split mCherry, using NEDD4-2 WW2 binding domain)DepositorTypeEmpty backboneUseTagsSplit mCherry system: NEDD4-2 WW2 fused to C-term…ExpressionBacterialMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-Ef1a-mCh-GFPi
Plasmid#31849DepositorInsertGFP RNAi
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterU6 for shRNA; Ef1alpha for mCherryAvailable sinceJan. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-Ef1a-mCh-Puro-Luci
Plasmid#31850DepositorInsertLuciferase RNAi
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterU6 for shRNA; Ef1alpha for mCherry-puromycinAvailable sinceJan. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-Cre stuffer v4
Plasmid#158033Purposelenti-viral construct with Cas9, Cre recombinase and U6 driven sgRNA casette with modified tracr RNA (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
psiCheck-DNAJA4CDS
Plasmid#41849DepositorInsertDNAJA4 CDS (DNAJA4 Human)
UseLuciferaseTagsrenilla luciferaseExpressionMutationStart codon of DNAJA4 removedPromoterAvailable sinceFeb. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
psiCheck-ApoECDS
Plasmid#41848DepositorInsertApoE CDS (APOE Human)
UseLuciferaseTagsrenilla luciferaseExpressionMutationPromoterAvailable sinceFeb. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Cre sgAdam10 v3
Plasmid#158034Purposelenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse Adam10DepositorInsertAdam10 sgRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
psiCheck-ApoE3'UTR
Plasmid#41846DepositorInsertApoE 3'UTR (APOE Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJan. 25, 2013AvailabilityAcademic Institutions and Nonprofits only