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Plasmid#105271PurposePlasmid for pop-in/pop-out replacement of Vps8 with Vps8-iGFPx6.DepositorInsertVps8 portion (VPS8 Budding Yeast)
UseTagsiGFPx6ExpressionYeastMutationPromoterAvailable sinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIMP1-Padc
Plasmid#90486PurposePlasmid with adc(CA_P0165) promoter for gene expression in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-APL5-iGFPx6
Plasmid#105252PurposePlasmid for pop-in/pop-out replacement of Apl5 with Apl5-iGFPx6.DepositorInsertApl5 (APL5 Budding Yeast)
UseTagsiGFPx6ExpressionYeastMutationPromoterAvailable sinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-msGFP-VPS21
Plasmid#105265PurposePlasmid for pop-in/pop-out replacement of Vps21 with msGFP-Vps21.DepositorInsertVps21 (VPS21 Budding Yeast)
UseTagsmsGFPExpressionYeastMutationPromoterAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-SNX4-iGFPx3
Plasmid#105269PurposePlasmid for pop-in/pop-out replacement of Snx4 with Snx4-iGFPx3.DepositorInsertSnx4 portion (SNX4 Budding Yeast)
UseTagsiGFPx3ExpressionYeastMutationPromoterAvailable sinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pREPK219H
Plasmid#51492PurposepREP with a mutation in the ATPase domain (K219H). This is predicted to result in loss of geminivirus replication.DepositorInsertRep/RepA
UsePlant expression t-dnaTagsExpressionMutationchanged lysine 219 to histidinePromoter2x35SAvailable sinceMay 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGALT-EGFP-IRES-puro2b
Plasmid#26463DepositorInsertpGalT
UseTagsEGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-T|C-iGFP-RCY1
Plasmid#105281PurposeOverexpresses iGFP-RCY1 from the TPI1 promoter. Integrating at TRP1.DepositorInsertRcy1 (RCY1 Budding Yeast)
UseTagsiGFPExpressionYeastMutationPromoterTPI1Available sinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJBM1
Plasmid#225288PurposeE. coli- C. coli shuttle vector; KanRDepositorInsertReplication elements from pWestmill 11
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-EF1a_NKX2-5
Plasmid#221491PurposeNKX2-5 lentiviral overexpressionDepositorInsertNKX2-5 (NKX2-5 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_NKX2-1
Plasmid#221472PurposeGateway cloning of NKX2-1 short isoform overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRegev_D3
Plasmid#204182PurposeSingle reporter with long guanine depleted (G-poor) region from splicing library.DepositorInsertExon D3
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTruncated CAGAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_D1.61C>G
Plasmid#204179PurposepRegev_D1 with single C>G mutation.DepositorInsertExon D1 with 61C>G mutation in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation61C>G mutationPromoterTruncated CAGAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_D2.30C>G
Plasmid#204181PurposepRegev_D2 with single C>G mutation.DepositorInsertExon D2 with 30C>G mutation in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation30C>G mutationPromoterTruncated CAGAvailable sinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_D4.34C>G
Plasmid#204185PurposepRegev_D4 with single C>G mutation.DepositorInsertExon D4 with 34C>G mutation in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation34C>G mutationPromoterTruncated CAGAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRegev_D3.48C>G
Plasmid#204183PurposepRegev_D3 with single C>G mutation.DepositorInsertExon D3 with 48C>G mutation in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation48C>G mutationPromoterTruncated CAGAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL1647
Plasmid#155148PurposeExpresses MlaF 1-246, taglessDepositorInsertmlaF(1-246)
UseTagsExpressionMutationPromoteraraBADAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL1840
Plasmid#155150PurposeExpresses MlaF-6xHisDepositorInsertMlaF-6xHis
UseTagsExpressionMutationPromoterT7 promoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL1266
Plasmid#155142PurposeExpresses MlaF, tagless, araBAD promoterDepositorInsertMlaF
UseTagsExpressionMutationPromoteraraBADAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
puC19 preACT1(del6) short BP-3'SS
Plasmid#111333PurposeT7 transcriptionDepositorInsertACT1
UseTagsExpressionBacterialMutationshorter branch to 3' splice site distancePromoterAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only