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pDAI-SceI

Plasmid

#32061

Purpose

Construction of unmarked gene deletions. Broad host range replicative plasmid expressing the I-SceI homing endonuclease

Depositor

Miguel Valvano

Article

Flannagan et al Environ Microbiol. 2008 Jun;10(6):1652-6

Insert

I-SceI nuclease

Use

Tags

Expression

Bacterial

Mutation

Promoter

Pdhfr

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pSC201plus

Plasmid

#164227

Purpose

Integrative plasmid to introduce modified rhamnose-inducible promoter into bacterial genomes. Harbours enhanced rhaS binding site and encodes T7 gene10 stem loop in mRNA for enhanced expression

Depositor

Silvia Cardona

Article

Hogan et al Appl Environ Microbiol. 2021 Jun 30:AEM0

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Rhamnose-inducible

Available since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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eNOS S1179D

Plasmid

#22484

Depositor

William Sessa

Article

Fulton et al Nature. 1999 Jun 10. 399(6736):597-601.

Insert

eNOS S1179D

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2009

Availability

Academic Institutions and Nonprofits only
YIplac204-pOst1-APVNTT-DsRed-Express2-FKBP(LV)C22V-QRPL

Plasmid

#140153

Purpose

Expresses a regulatable vacuolar cargo for yeast at the TRP1 locus

Depositor

Benjamin Glick

Article

Casler et al Elife. 2020 Jun 25;9. pii: 56844. doi: 1

Insert

pOst1-APVNTT-DsRed-Express2-FKBP(LV)C22V-QRPL

Use

Tags

Expression

Yeast

Mutation

Promoter

TPI1

Available since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pJA327

Plasmid

#74486

Purpose

superfolder GFP (myo-3::sfGFP::let-858, using backbone as in L3785)

Depositor

Andrew Fire

Article

Arribere et al Nature. 2016 Jun 1;534(7609):719-723. do

Insert

superfolder GFP

Use

Tags

Expression

Worm

Mutation

Promoter

myo-3

Available since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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pAC1804_pmax-dCasRx-RBM38

Plasmid

#118638

Purpose

Transient transfection; Expresses dCasRx-RBM38; CAGGS promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

dCasRx-RBM38

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCJH019_SC101_pLAC_Cas12c(4)_Kan

Plasmid

#183071

Purpose

Wild-type Cas12c_4 in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Cas12c_4

Use

Tags

Expression

Bacterial

Mutation

Wild-type

Promoter

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pcNB2

Plasmid

#89242

Purpose

Resurfaced Cell-penetrating 5F7 nanobody

Depositor

Brian McNaughton

Article

Bruce et al Protein Sci. 2016 Jun;25(6):1129-37. doi

Insert

pcNB2 (5F7)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2T

Plasmid

#15808

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2T (UBE2T Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-wtVHL

Plasmid

#13390

Depositor

Lucy Anderson

Article

Shiao et al Cancer Res. 2000 Jun 1. 60(11):2816-9.

Insert

von Hippel-Lindau (Vhl Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2006

Availability

Academic Institutions and Nonprofits only

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EGFP-hSV1_WT

Plasmid

#129483

Purpose

Mammalian expression of full-length human supervillin, isoform 1.

Depositor

Elizabeth Luna

Article

Smith et al Cytoskeleton (Hoboken). 2010 Jun;67(6):3

Insert

Supervillin (SVIL Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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PCS2+ Cas9

Plasmid

#122948

Purpose

For in vitro transcription to produce Cas9 mRNA for microinjection into mouse embryos

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Cas9

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

SP6

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pUC18-mini-Tn7T

Plasmid

#64954

Purpose

mini-Tn7 base vector with transcriptional terminator

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pRS405 VAC8-GFP

Plasmid

#166836

Purpose

Expresses Vac8-GFP in yeasts.

Depositor

Daniel Klionsky

Article

Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

Insert

VAC8 (VAC8 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

VAC8 promoter

Available since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGCDNsam-Foxa1

Plasmid

#33003

Depositor

Atsushi Suzuki

Article

Sekiya et al Nature. 2011 Jun 29;475(7356):390-3. doi

Insert

forkhead box A1 (Foxa1 Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2012

Availability

Academic Institutions and Nonprofits only
CKAR-T/A

Plasmid

#14861

Depositor

Alexandra Newton

Article

Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Insert

C kinase activity reporter

Use

Tags

CFP and YFP

Expression

Mammalian

Mutation

T/A

Promoter

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsTNRC6Biso1

Plasmid

#25034

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

TNRC6B (TNRC6B Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2010

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2E3

Plasmid

#15789

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2E3 (UBE2E3 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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EasyFusion mAID-mCherry with Neo

Plasmid

#113831

Purpose

EasyFusion vector for targeting endogeneous genes with mAID-mCherry

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

mAID-mCherry

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pUCSh5vio-2300

Plasmid

#170987

Purpose

Encodes sgRNA target 2300 and Gentamicin resistance - violacein pathway transposon.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

Sh-CAST sgRNA 2300 violacein

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

placI

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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