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pSR21
Plasmid#69156Purposeread-outlox2272 mCherry to tagBFP switch for integration on ttTi14024, Chr XDepositorInsertsmCherry
TagBFP
UseCre/Lox; MossciTagsExpressionWormMutationcodon-optimzed index 1.0Promoterrps-27Available sinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUB_SM-RLuc0---MS2
Plasmid#210572PurposeExpresses SM(FLAG)-nonoptimal Renilla with 3'UTR MS2 hairpinsDepositorInsertSpaghetti monster (FLAG)-nonoptimal Renilla luciferase
UseLentiviral and LuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterUBC humanAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_SM-FLuc0-malat1
Plasmid#210580PurposeExpresses SM(FLAG)-nonoptimal Firefly - 3'UTR malat1 triple helixDepositorInsertSpaghetti monster (FLAG)-nonoptimal FLuc - 3'UTR malat1 triple helix
UseLuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
LC8-like
Plasmid#133115PurposeHuman LC8-like protein, codon optimised for Sf9 expression.DepositorInsertLC8-like (LOC392067 Human)
UseTagsExpressionInsectMutationCodon optimised for Sf9 expressionPromoterAvailable sinceOct. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB123 - pL0_rLUC-I (CDS1)
Plasmid#123181PurposeGolden Gate (MoClo; CDS1) compatible luciferase gene from Renilla reniformis with an intron for transient and stable expression in plantsDepositorInsertluciferase gene from Renilla reniformis with an plant intron and codon optimized for plants
UseLuciferase; Part for plant expressionTagsExpressionPlantMutationNo BpiI and BsaI sites; codon-optimised for plantsPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCH1/RAD51o
Plasmid#102562PurposeExpress Human RAD51 protein codon optimzed for E.coli and the E.coli GorE OperonDepositorInsertsHomo sapiens RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) (RAD51), transcript variant 1 (RAD51 Synthetic, Human)
E. coli groE operon
UseTagsExpressionBacterialMutationCodon optimized for E.coli ExpressionPromoterT7Available sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOPINB-hRNF216
Plasmid#167354PurposeExpression of human RNF216 (Triad3A) codon optimized for E. coli and insect cellsDepositorInsertE3 ubiquitin-protein ligase RNF216 (RNF216 Human)
UseTags3C protease cleavage site and 6x-His tagExpressionBacterialMutationcodon optimised for expression in E. coliPromoterT7Available sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
RV-Cag-Dio-EYFP-P2A-cMaf
Plasmid#98174PurposeRetroviral vector encoding Cre-dependent expression of EYFP-P2A-MafDepositorInsertcMaf (codon optimzed) (Maf Mouse)
UseRetroviralTagsExpressionMutationCodon optimizedPromoterCAGAvailable sinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOPINB-hRNF216(457-784)
Plasmid#171922PurposeExpression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells. Catalytic helix-RBR-helix construct suitable for activity assays.DepositorInsertE3 ubiquitin-protein ligase RNF216 (RNF216 Human)
UseTags3C protease cleavage site and 6x-His tagExpressionBacterialMutationcodon optimised for expression in E. coliPromoterT7Available sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pdCas9 (GB1079)
Plasmid#75399PurposeProvides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part for C-terminal fusionsDepositorInsertCas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOPINB-hRNF216(511-784)
Plasmid#171923PurposeExpression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells. Catalytic RBR-helix construct suitable for activity assays.DepositorInsertE3 ubiquitin-protein ligase RNF216 (RNF216 Human)
UseTags3C protease cleavage site and 6x-His tagExpressionBacterialMutationcodon optimised for expression in E. coliPromoterT7Available sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDN-D2irTN6kwh
Plasmid#44724DepositorInsertspCMV-D2i promoter
htetR::NLS
UseSynthetic Biology; Expression regulator/reporter;…TagsRabbit β-globin intron II and WPREExpressionMammalianMutationInitiator motif (Inr) displaced relative to pCMV-…PromoterpCMV-D2iAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pESC-LEU-HsAIDSc
Plasmid#60810Purposeto express human AID recoded for yeast codon usageDepositorInsertAID (AICDA Human)
UseTagsMycExpressionYeastMutationcodon optimized for yeastPromoterGAL1Available sinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGB 35S:dCas9:Tnos (GB1191)
Plasmid#68223PurposeTranscriptional unit for human codon optimized with mutated (D10A, H840A) and inactivated catalytic domains Cas9 protein plant expression driven by the 35S promoterDepositorInsertdCas9
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removed; human codon optimis…Promoter35SAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::UMSBP::sNluc2_ORF1
Plasmid#186765PurposeAn mRNA production vector encoding planarian codon-optimized sNluc2 with UMSBP 5' and 3' UTRs beginning at the endogenous gene's start codon.DepositorInsertNanoluciferase
UseTagsExpressionMutationPromoterT7Available sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-CH111-QES.c13.L544Y-754*
Plasmid#123264PurposeMammalian expression plasmid for Env from the CH111 HIV-1 isolate; C-terminal truncation; QES mutant for enhanced presentation of quaternary epitopesDepositorInsertHIV-1 (AD8) Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic gene; Premature stop co…PromoterCMVAvailable sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-AD8-QES.i08.c04-754*
Plasmid#123258PurposeMammalian expression plasmid for Env from the AD8 HIV-1 isolate; C-terminal truncation; QES mutant for enhanced presentation of quaternary epitopesDepositorInsertHIV-1 (AD8) Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic gene; Premature stop co…PromoterCMVAvailable sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-Q769-QES.i03.c04-754*
Plasmid#123228PurposeMammalian expression plasmid for Env from the Q769.d22 HIV-1 isolate; C-terminal truncation; QES mutant with enhanced binding to PG16DepositorInsertHIV-1 (Q769.d22) Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic gene; Premature stop co…PromoterCMVAvailable sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-BaL-QES.i01.c08-754*
Plasmid#123213PurposeMammalian expression plasmid for Env from the BaL HIV-1 isolate; C-terminal truncation; QES mutant with enhanced binding to PG16DepositorInsertHIV-1 (BaL) Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic gene; Premature stop co…PromoterCMVAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only
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