Addgene

We narrowed to 5,544 results for: KRAS

Showing: 1701 - 1720 of 5544 results

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pENTR221-WNT3A

Plasmid

#79501

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human WNT3A CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

WNT3A (WNT3A Human)

Use

Multisite gateway entry clone

Tags

Expression

Mutation

Promoter

no Promoter

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pLenCMV-PMMars1CyGFP

Plasmid

#78315

Purpose

Lentiviral transfer vector encoding Mars1Cy-GFP membrane-spanning protein

Depositor

Alan Waggoner

Article

Zhang et al J Clin Invest. 2015 Oct 1;125(10):3915-2

Insert

Mars1Cy-TMD-GFP

Use

Lentiviral

Tags

HA epitope

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 1, 2016

Availability

Academic Institutions and Nonprofits only

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p7012 MSCV-IP N-HAonly 18E6nonsplice

Plasmid

#44148

Depositor

Peter Howley

Article

White et al J Virol. 2012 Dec;86(24):13174-86. doi:

Insert

HPV18E6

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Internal splice site mutated

Promoter

MSCV LTR

Available since

April 30, 2013

Availability

Academic Institutions and Nonprofits only
pAM-PAT-ProTTG1 GW

Plasmid

#79753

Purpose

binary DEST vector for plant expression

Depositor

Nico Dissmeyer, Martin Hulskamp

Article

Bouyer et al PLoS Biol. 2008 Jun 10;6(6):e141. doi: 1

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

ProTTG1

Available since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR221-CMV.Pro

Plasmid

#79486

Purpose

MultiSite Gateway entry clones, first fragment, (attL1, attR5), CMV promoter

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

CMV Promoter

Use

Multisite gateway entry clone

Tags

Expression

Mutation

Promoter

CMV Promoter

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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miR373 in pcDNA3.1

Plasmid

#78127

Purpose

Mammalian expression of miR373

Depositor

Heidi Schwarzenbach

Article

Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

Insert

miR373 (MIR373 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pGMCKq20-T38S38-0X

Plasmid

#49522

Purpose

Kanamycin resistant; contains qtag20 and constitutively expresses reverse TetR (tetR38); integrates into the mycobacteriophage att-L5 site

Depositor

Dirk Schnappinger

Article

Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

Insert

constitutive TetR38

Use

Tags

Expression

Bacterial

Mutation

Promoter

Ptb38

Available since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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p7013 MSCV-IP N-HAonly 45E6nonsplice

Plasmid

#44146

Depositor

Peter Howley

Article

White et al J Virol. 2012 Dec;86(24):13174-86. doi:

Insert

HPV45E6

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Internal splice site mutated.

Promoter

MSCV LTR

Available since

April 30, 2013

Availability

Academic Institutions and Nonprofits only
FLINC-AKAR1-CAAX

Plasmid

#87705

Purpose

Fluorescent biosensor for super-resolution imaging of PKA activity; targeted to plasma membrane.

Depositor

Jin Zhang

Article

Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

Insert

FLINC-AKAR1-CAAX

Use

Tags

C-terminal targeting sequence from Kras

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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PKAsub-TagRFPT-CAAX

Plasmid

#87712

Purpose

Encodes C-terminal (substrate) fragment of bimolecular super-resolution PKA activity reporter (bimFLINC-AKAR); targeted to plasma membrane; use in conjunction with Dronpa-FHA1 (Addgene #87711).

Depositor

Jin Zhang

Article

Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

Insert

PKAsub-TagRFPT-CAAX

Use

Tags

6xHis, C-terminal targeting sequence from Kras, T…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) K-Ras(WT)

Plasmid

#224283

Purpose

Cell transfection and expression of K-Ras

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

K-Ras (KRAS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) K-Ras(G12C)

Plasmid

#224284

Purpose

Cell transfection and expression of K-Ras (G12C)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

K-Ras (KRAS Human)

Use

Tags

Expression

Mammalian

Mutation

G12C

Promoter

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pLVET-HA-K-RasG12V-IRES-GFP

Plasmid

#107140

Purpose

A bicistronic lentiviral mammalian expression vector for HA-KRasG12V(4B) and GFP

Depositor

Aki Manninen

Article

Zhang et al Oncogene. 2017 Oct 12;36(41):5681-5694.

Insert

HA-tagged Kras4B(G12V) and GFP (KRAS Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hEF-1alpha

Available since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pBS1C

Plasmid

#55168

Purpose

Empty vector, integration at amyE, ampr, cmr

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Bacillus biobrick box

Tags

Expression

Mutation

Promoter

Available since

Aug. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Cdc42(Q61L)

Plasmid

#12600

Depositor

Klaus Hahn

Article

Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

Insert

EGFP-Cdc42 (Q61L) (CDC42 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Q61L

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgRNA

Plasmid

#124845

Purpose

Vector for FLP-dependent expression of SaCas9 with gRNA

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

SauCas9 gRNA scaffold

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-cGAS-HA

Plasmid

#130910

Purpose

Expresses human cGAS-HA; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

cGAS

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCPP6450

Plasmid

#128731

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Wei et al Cell Host Microbe. 2015 Jun 10;17(6):752

Insert

HopAD1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-SAMTOR

Plasmid

#100508

Purpose

Expresses FLAG tagged SAMTOR in mammalian cells

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

SAMTOR (BMT2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-CMV-Rheb(S16H)-IRES-eGFP-W

Plasmid

#32520

Depositor

Mustafa Sahin

Article

Nie et al Nat Neurosci. 2010 Feb;13(2):163-72. Epu

Insert

Constitutively active Rheb (S16H) (Rheb Rat)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

S16H to make constitutively active

Promoter

CMV

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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