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GFP-ATG8(416)/GFP-AUT7(416)
Plasmid#160389PurposeExpresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter , NAT selectionDepositorInsertautophagy-related 8 (ATG8 Budding Yeast)
UseCen/arsTagsExpressionYeastMutationPromoterEndogenous ATG8 promoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKrox24(MapERK)d1EGFP (KroxDs)
Plasmid#214912Purposefor PiggyBac mediated integration and stable expression of destabilised EGFP as a reporter to MAPK/ERK pathway activationDepositorInsertd1EGFP under the MapERK promoter
UseTagsExpressionMammalianMutationPromoterMapkERK promoterAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
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p5E-mitfa2.1
Plasmid#81234Purposezebrafish mitfa promoter entry vector for multisite Gateway cloningDepositorInsertmitfa promoter (mitfa Zebrafish)
UseMultisite gateway entry vectorTagsExpressionMutationPromotermitfa, 2.1kb including 5' UTRAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
plenti guide ds red
Plasmid#128055PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and DsREd from EF-1a promoter. 3rd generation lentiviral backbone.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
isoform PKC Gamma (JCE692)
Plasmid#89780Purposeyeast expression of mouse PKC Gamma with yeast PKC1 promoter and ADH1 terminatorDepositorInsertPKC Gamma (Prkcg Mouse)
UseTagsExpressionMammalianMutationPromoteryeast PKC1 promoter (-600 to -1)Available sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_TDH3
Plasmid#166084PurposePlasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of TDH3 for double stranded break formation in yeast.DepositorInsertPromoter of TDH3 (TDH3 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_PROX1_1+4
Plasmid#121177PurposeLentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.DepositorInsertCas9-P2A-puro and U6 promoters driven expression of gRNAs
UseCRISPR and LentiviralTagsP2A-puroExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-gltA2-udhA
Plasmid#65819PurposegltA-udhA targeting guide RNA array E. coli , Low Phosphate InductionDepositorInsertgltA2-udhA guide array
UseTagsExpressionBacterialMutationPromoterE . coli ugpB gene promoter, low phosphate induc…Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX552-GFP
Plasmid#107023PurposepX552-CMV-GFP. AAV plasmid for sgRNA cloning, with GFP fusion facilitating FACS sorting of cells. Replaced the hSyn promoter in pX552 from Zhang lab with a CMV promoter.DepositorInsertGFP
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPROBE'-gfp[tagless]
Plasmid#40165DepositorInsertPromotor probe
UseTagsExpressionMutationPromoterAvailable sinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-PEmax-P2A-GFP
Plasmid#202000PurposeBased on the pCMV-PEmax-P2A-GFP, we replaced the CMP promoter with the EF1a promoter for expression in iPSC.DepositorInsertPEmax-P2A-GFP
UseCRISPR and Synthetic BiologyTagsGFPExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-RLuc
Plasmid#83282Purposerecombinant AAV vector packaging single-stranded Renilla Luciferase under the CAG promoter (includes WPRE element)DepositorInsertRenilla Luciferase
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPROBE-gfp[LVA]
Plasmid#40170DepositorInsertPromotor probe
UseTagsExpressionMutationPromoterAvailable sinceDec. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPROBE-AT
Plasmid#37812DepositorInsertPromotorless gfp reporter gene
UseTagsExpressionMutationPromoterAvailable sinceJuly 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPROBE-gfp[tagless]
Plasmid#40164DepositorInsertPromotor probe
UseTagsExpressionMutationPromoterAvailable sinceDec. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAGM4723:Ble:Cas9
Plasmid#112208PurposeGolden Gate Level 2 construct encoding Cas9-YFP, Zeocin resistance.DepositorInsertsCas9
SheBle
UseCRISPR; Diatom, phaeodactylumTagsPhaeodactylum fcp promoter and terminator and YFPExpressionMutationPhaeodactylum Fcp promoterPromoterPhaeodactylum FcpAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPROBE'-gfp[LVA]
Plasmid#40171DepositorInsertPromotor probe
UseTagsExpressionMutationPromoterAvailable sinceDec. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPROBE-gfp[ASV]
Plasmid#40166DepositorInsertPromotor probe
UseTagsExpressionMutationPromoterAvailable sinceDec. 6, 2012AvailabilityAcademic Institutions and Nonprofits only
