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Plasmid#109421PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCherry. VHH-mCherry also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, mCherry, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, and T7ExpressionBacterialMutationPromoterT7Available sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28_mouse-pro-IL1B
Plasmid#183386PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorInsertpro-IL1B (Il1b Mouse)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET30a-SARS-CoV2-NP
Plasmid#165090Purposeprotein expression in E. coliDepositorInsertSevere acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal (N SARS-CoV2)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 10, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-IMX-DogTag
Plasmid#113765PurposeExpresses IMX-DogTag in bacterial cells for modular antigen multimerization using SnoopLigaseDepositorInsertIMX-DogTag
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET51b-NAD-cpLuc2
Plasmid#135629PurposeBacterial expression of a biosensor for measuring NAD+ levels at the point of care (V2)DepositorInsertNAD-cpLuc2 sensor
UseTagsHisx10 and StrepExpressionBacterialMutationPromoterT7Available sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET24b-SUMO-BG2.4
Plasmid#199438Purposeexpresses BAmGreen2.4 in bacterial cells with a SUMO tagDepositorInsertBAMGreen2.4
UseTagshis tag and sumoExpressionBacterialMutationPromoterT7Available sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETCON-PTX-S1-220
Plasmid#67747PurposePertussis Toxin Subunit 1 Yeast Display PlasmidDepositorInsertPTxS1-220
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
HM3C NaVAe1_p pET24b
Plasmid#127854PurposeBacterial expression vector for NaVAe1 bacterial sodium channel residues 143-288. It expresses HIStag-MBP-3C-channel protein.DepositorInsertNaVAe1p
UseTags3C Cleavage site, His, and MBPExpressionBacterialMutationPromoterAvailable sinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_01
Plasmid#194662PurposeProtein production of the split HaloTag based calcium recorder Caprola_01 in bacteriaDepositorInsertCaprola_01
UseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-RBP-LOV2
Plasmid#185792PurposeEncodes Light-oxygen-voltage-sensing domain 2 linked to RNA binding peptide (on N terminal end) via GS-linkerDepositorInsertRBP-LOV2
UseTags10xHis, TEV protease cleavage site after His TagExpressionBacterialMutationPromoterT7Available sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET22b-His6-UBCH7
Plasmid#212711PurposeBacterial expression of His6-UBCH7DepositorInsertUBCH7
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-Cre-6xHis
Plasmid#62939PurposeExpression of Cre-6xHis in bacterial of cellsDepositorInsertCre recombinase
UseTags6xHis tagExpressionBacterialMutationPromoterT7Available sinceJune 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-DUET-1-mRhubarb713-HO
Plasmid#141199PurposeExpresses mRhubarb713 fluorescent protein and heme oxygenaseDepositorInsertsmRhubarb713
heme oxygenase
UseTags6x-HisExpressionBacterialMutationR134H, W309R, Q310A compared to iRFPPromoterT7Available sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET 2C-T10-dCasX
Plasmid#126418PurposeE. coli expression vector for inactive casX (triple mutations)--Used for CasX protein purificationDepositorInsertDpbCasX
UseCRISPRTagsHis-MBPExpressionBacterialMutationPromoterAvailable sinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-His-MinD_MinE
Plasmid#133621PurposeExpression vector for the Escherichia coli MinD with an N-terminal His-tag for purification. MinE is encoded in the ORF to avoid toxicity when overexpressing MinD, but MinE is not tagged.DepositorUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET42a-lambdaN+-L+-GSH
Plasmid#98894PurposeExpresses lambdaN+-L+-GST fusion protein for affinity purification with ARiBo methodDepositorInsertlambdaN+-L+
UseTagsGST tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceAug. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-mCherry
Plasmid#145279PurposeBacterial Expression of 6xHis mCherry Fusion ProteinDepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MH6-EsCas13d
Plasmid#108303PurposeExpresses E. coli codon optimized EsCas13d in the pET28a backbone.DepositorInsertEsCas13d
UseCRISPRTagsmH6 tagExpressionBacterialMutationPromoterlacAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-NLS-M-MLV-RT
Plasmid#181804PurposepET28a-NLS-M-MLV RTDepositorInsertNLS-M-MLV-RT
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SUMO-DogTag
Plasmid#105629PurposePlasmid for recombinant expression in bacteria of SUMO domain fused to DogTag, permitting covalent linkage to SnoopTagJrDepositorInsertSUMO-DogTag
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only