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Showing: 1681 - 1700 of 4392 results

  7. pPW3783 : CMVd3-ERmembrane-GST-2xHaloTag-KKMP

    Plasmid
    #185687
    Purpose
    Low-level transient expression of two tandem HaloTag proteins targeted to the ER membrane, fused with a GST for constitutive dimerization (making a HaloTag tetramer).
    Depositor
    Peter Walter
    Article
    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75
    Insert
    ERmembrane-GST-2xHaloTag-KKMP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    185687-plasmid-map-sequence-id-366415

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  14. pET28a(+)::MBP-NL3F10H

    Plasmid
    #141291
    Purpose
    MBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)
    Depositor
    Andrew Millar
    Article
    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10
    Insert
    MBP-NLUC3F10H
    Use
    Luciferase
    Tags
    Flag (x3), His (x10), and MBP
    Expression
    Bacterial
    Mutation
    Engineered for high stability (t1/2 = 11.5 days a…
    Promoter
    promoter for bacteriophage T7 RNA polymerase
    Available since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    141291-plasmid-map-sequence-id-324616

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  16. pET28a Gx-NL

    Plasmid
    #169105
    Purpose
    Expresses protein G fused to NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.
    Depositor
    Maarten Merkx
    Article
    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66
    Insert
    protein G - NanoLuc
    Use
    Tags
    his-tag and strep-tag
    Expression
    Bacterial
    Mutation
    Ala 24 stop
    Promoter
    T7
    Available since
    June 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    169105-plasmid-map-sequence-id-335658

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  20. pDNA3-Cerulean-FHA1-NES

    Plasmid
    #138202
    Purpose
    Encodes N-terminal (PAABD) fragment of FRET-based bimolecular kinase activity reporters (BimKARs); cytosol targeted.
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    Cerulean-FHA1-NES
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    138202-plasmid-map-sequence-id-307221

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Showing: 1681 - 1700 of 4392 results