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Plasmid#154833PurposeDonor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing one copy of etanerceptDepositorInsertETN
UseCre/LoxTagsExpressionMammalianMutationPromoter100RPU.2Available sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-TgKI-MCS-p2A-mScarlet-MCS-polyA
Plasmid#174029PurposeThis plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.DepositorInsertp2A-mScarlet
UseZebrafish knock-in taggingTagsExpressionMutationPromoterAvailable sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-166:CDH1-mEGFP
Plasmid#193921PurposeHomology arms and LE-mEGFP sequence for C-terminus tagging of human cadherin 1DepositorInsertcadherin 1 Homology Arms with LE-mEGFP (CDH1 Human)
UseAAV and CRISPR; Donor templateTagslinker-mEGFPExpressionMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceDec. 22, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC19-TgKI-MCS-mScarlet-MCS-polyA
Plasmid#174023PurposeThis plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.DepositorInsertmScarlet
UseZebrafish knock-in taggingTagsExpressionMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-079:DCP1A-mEGFP
Plasmid#193922PurposeHomology arms and mEGFP-SRA for N-terminus tagging of human DCP1ADepositorInsertDCP1A Homology Arms with mEGFP-SRA (DCP1A Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceDec. 22, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBA790
Plasmid#186245Purposednap-C HR Donor Vector (T4 phage editing)DepositorInsertBBR1 backbone + T4 phage homology
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC1812_pCR8-gCasRx-CUG (control gRNA)
Plasmid#118645PurposeTransient transfection; Expressed Control (CUG repeat) CasRx gRNA; Gateway DonorDepositorInsertgCasRx-CUG
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pR26-CAG-H2B-miRFP703
Plasmid#112853PurposeDonor vector for targeting H2B-miRFP703 into Rosa26 locus to generate reporter mice or cell linesDepositorInsertH2B-miRFP703
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
mUAVbla:tetR 5′
Plasmid#149379PurposemUAVbla containing a tetracycline resistance (tetA) cassette for building Gene Doctoring donor plasmids from pDOC-GG, using BsaI Golden Gate assembly with GGAG and CGCT overhangsDepositorInserttetA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHACK-GAL4>QF2(newHA1)
Plasmid#104873PurposeHACK Donor plasmids for converting GAL4 lines to QF2 lines using HACK method. Updated version of Addgene#80275 for easier cloning of insertsDepositorInsertT2A-QF2
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGT-Ah3
Plasmid#122570PurposeMAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)DepositorInsertbeta-lactamase/sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTopo pLox hPGK-Puro pA pLox
Plasmid#171048PurposeEmpty donor template for CRISPR/Cas9 targeting of TERT RE'sDepositorTypeEmpty backboneUseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBA789
Plasmid#186244Purposesoc-F HR Donor Vector (T4 phage editing)DepositorInsertBBR1 backbone + T4 phage homology
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR macroH2A1.2-C
Plasmid#30512DepositorInsertmacroH2A1.2-C (Macroh2a1 Mouse)
UseGateway - donor vectorTagsExpressionMutationNO STOPPromoterAvailable sinceJune 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDONR-SUMO1
Plasmid#114388PurposepDONR vector with SUMO1 geneDepositorInsertSUMO1 (SUMO1 Human)
UseTagsExpressionMammalianMutationQ29H compared with NCBI reference NP_003343.1PromoterAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
H52-pUCM-hNGN2-inv
Plasmid#110492PurposeDonor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron, TALEN cut site outside of homology armDepositorInsertsUseCRISPR, Cre/Lox, and TALENTagsExpressionMammalianMutationPromoterCAG, EF-1 alpha, and TRE3GAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
LT2_12_GlyRNA
Plasmid#177710PurposePlasmid for genome integration in X2 site expressing GlyRNA (glycolytic flux biosensor)DepositorInsertspTEFmut8-mTurquoise2-RNA_2.6--tCYC1
pTEFmut8-mCherry-tADH1
UseUsed as donor dna in genome integration after lin…TagsExpressionYeastMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
Syt1LeftArm-3XGS-GCaMP6s-SV40-3XP3-dsRed-SV40-Syt1RightArm
Plasmid#159636PurposeDonor plasmid for generating Syt1:GCaMP6s strain in Aedes aegypti with CRISPR/Cas9DepositorInsertGCaMP6s
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
LT2_7_OxPro
Plasmid#177709PurposePlasmid for genome integration in X2 site expressing OxPro (oxidative stress biosensor)DepositorInsertspTRX2_5xUAS-ymYPET-tCYC1
pTEFmut8-mCherry-tADH1
UseUsed as donor dna in genome integration after lin…TagsExpressionYeastMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGT-Ah2
Plasmid#122569PurposeMAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)DepositorInsertbeta-lactamase/sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only