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Showing: 1681 - 1700 of 3013 results

  9. pORANGE_NFL linker(A6TAG)-3xFLAG KI

    Plasmid
    #182680
    Purpose
    Expression of spCas9, gRNA targeting the end of mouse Nefl gene and donor linker(A6TAG)-3xFLAG. Can be used for amber codon suppression and click chemistry labeling of endogenous NFL.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    Nefl-targeting gRNA and linker(A6TAG)-3xFLAG donor sequence (Nefl Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and chicken beta-actin promoter
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182680-plasmid-map-sequence-id-363164

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  10. pExpreS2-1-CR-PA

    Plasmid
    #175445
    Purpose
    HiFi-compatible destination vector for secreted overexpression of thrombin-cleavable, C-terminal Protein A tagged proteins with ExpreS2 Platform
    Depositor
    Wyatt Yue
    Article
    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8
    Type
    Empty backbone
    Use
    Tags
    BiP Secretion Peptide and Thrombin-Protein A
    Expression
    Insect
    Mutation
    Promoter
    Fused Actin-HSP70
    Available since
    March 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    175445-plasmid-map-sequence-id-358137

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  13. pABD1031

    Plasmid
    #68004
    Purpose
    Protein co-localization of PIF1 (PIL5) via fluorescent fusion after transient expression in planta
    Depositor
    Bruce Downie
    Article
    Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;11
    Insert
    PHY-INTERACTING FACTOR 1 (PIF1) (PIL5 )
    Use
    Tags
    eYFP
    Expression
    Plant
    Mutation
    Promoter
    2X Cauliflower Mosaic Virus 35S promoter
    Available since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only

  14. pABD1275

    Plasmid
    #68017
    Purpose
    PIF1 protein overproduction for purification from bacterial cells
    Depositor
    Bruce Downie
    Article
    Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;11
    Insert
    PHY-INTERACTING FACTOR 1 (PIF1) (PIL5 Mustard Weed, AT2G20180)
    Use
    Tags
    6x-His
    Expression
    Bacterial
    Mutation
    Codon Optimized for bacterial expression (GenScri…
    Promoter
    T7
    Available since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only

  16. pcDNA3/mStayGold(c4)=UtrCH

    Plasmid
    #212020
    Purpose
    For filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ‘=’).
    Depositor
    Atsushi Miyawaki
    Article
    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s
    Insert
    UtrCH
    Use
    Tags
    mStayGold(c4)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    212020-plasmid-map-sequence-id-419202

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  17. pHLmMBP-8

    Plasmid
    #72347
    Purpose
    Mammalian expression of cytoplasmic mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag
    Depositor
    Luca Jovine
    Article
    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii
    Type
    Empty backbone
    Use
    Tags
    6His-tag, HA-tag, Strep-tag II, and mMBP with C-t…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV enhancer + chicken beta-actin promoter
    Available since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    72347-plasmid-map-sequence-id-133755

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  19. pHLmMBP-1

    Plasmid
    #72343
    Purpose
    Mammalian expression of secreted mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag
    Depositor
    Luca Jovine
    Article
    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii
    Type
    Empty backbone
    Use
    Tags
    6His-tag, HA-tag, Strep-tag II, and mMBP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV enhancer + chicken beta-actin promoter
    Available since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    72343-plasmid-map-sequence-id-132066

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  20. MultiMate 5 colors

    Plasmid
    #206266
    Purpose
    Expression of 5 fluorescently labelled proteins in mammalian cells. Can be used as a CRE-donor (MultiBac system)
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    H2B (H. Sapiens), Actin and Tubulin (B. taurus), mito mCherry (Synthetic), CyOFP1 (E. quadricolor)
    Use
    Recombinant baculovirus production, cre acceptor…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206266-plasmid-map-sequence-id-406652

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Showing: 1681 - 1700 of 3013 results