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Plasmid#132623PurposeExpresses a TurboRFP transcript with test microRNA sponge for mmu-miR-292b-3p as 3'UTR; serves as template for other microRNA spongesDepositorInsertstop codon, mmu-miR-292b-3p sponge sequence and polyA signal
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-chGrb2/eDHFR(69K6)-iRFP713
Plasmid#178854PurposeMammalian expression of Grb2-eDHFR(69K6) chimera fused to iRFP713DepositorInsertGrb2(1-59)-eDHFR(69K6)-Grb2(152-216)-iRFP713 (GRB2 Human)
UseTagsExpressionMammalianMutationGrb2: Y160EPromoterCAGAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-chPKCdelta/eDHFR(69K6)-miRFP670
Plasmid#178858PurposeMammalian expression of PKCδ-eDHFR(69K6) chimera fused to miRFP670DepositorInsertPKCδ(1-229)-eDHFR(69K6)-PKCδ(280-675)-miRFP670 (PRKCD Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-miRFP670-eDHFR(69K6)-G-alpha-s
Plasmid#172105PurposeMammalian expression of Gαs fused to miRFP670-eDHFR(69K6)DepositorInsertmiRFP670-eDHFR(69K6)-Gαs (GNAS Human)
UseTagsExpressionMammalianMutationGαs: C3SPromoterCAGAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
PX552-rScn2a-3xV5-Syn-miRFP670
Plasmid#182566PurposeFor rat Nav1.2 knockin with 3xV5 at the extracellular loopDepositorInsertsrat Scn2a gRNA and 3xV5 donor DNA
miRFP670
UseAAV and CRISPRTagsExpressionMutationPromoterSyn and U6Available sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT2Aneo-mRFP-FKBP-mSos1-linkercat
Plasmid#173858PurposeEncoding a part of the rapamycin-induced Ras activation system.DepositorInsertmRFP-FKBP-mSos1-linkercat
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
C232-E34: attB2r-iRFP670-attB3
Plasmid#162908PurposeGateway attB2r/attB3 entry clone for construction of C-terminal iRFP670 fusion proteinsDepositorInsertfar-red iRFP670 fluorescent protein
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-JNKKTR -miRFP670-WPRE
Plasmid#159976Purposesingle wavelength translocating reporter of the JNK pathwayDepositorInsertJNKKTR -miRFP670
UseAdenoviralTagsExpressionMammalianMutationsyntheticPromoterhSyn1Available sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-105 GFP(1-9)-IRES-iRFP670
Plasmid#130124PurposePPI (BiFC/TriFC) assay vectorDepositorInsertGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-106 GFP(1-9)-IRES-iRFP702
Plasmid#130125PurposePPI (BiFC/TriFC) assay vectorDepositorInsertGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Prham-tagRFP-IMBB2.4-pUC57-mini
Plasmid#149343Purposeexpresses tagRFP fluorescent protein in E. coliDepositorInserttagRFP
UseTagsExpressionBacterialMutationPromoterrhamnoseAvailable sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti4 Blast RFP-PNRC1 W300A
Plasmid#123298PurposeMammalian expression of PNRC1-W300A mutant N-RFPDepositorInsertPNRC1 (PNRC1 Human)
UseLentiviralTagsturboRFPExpressionMammalianMutationW300APromoterAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBMN iRFP670-OPTN(F178A/D474N)
Plasmid#119694PurposeFor mammalian expression of iRFP-tagged LIR/UBD double mutant OPTNDepositorInsertOPTN (OPTN Human)
UseRetroviralTagsiRFP670ExpressionMammalianMutationF178A/D474NPromoterAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBMN iRFP670-NDP52(V136S/C443K)
Plasmid#119696PurposeFor mammalian expression of iRFP-tagged LIR/UBD double mutant NDP52DepositorInsertNDP52 (CALCOCO2 Human)
UseRetroviralTagsiRFP670ExpressionMammalianMutationV136S/C443KPromoterAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tol2-lyzC-RFP-miR-30 backbone
Plasmid#97147PurposeAs a control of miRNA expressionDepositorTypeEmpty backboneUseZebrafishTagsExpressionMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
YCe2764 HC_Kan_RFP-Connector entry vector
Plasmid#100636PurposeEMMA toolkit connector-entry vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJM103: 150bp Hps2 RFP on pCM66T
Plasmid#74744PurposepCM66T with shortened pHps2 promoter driving RFP expression (maxRFP = BBa_E1010)DepositorInsertRFP (BioBrick part number BBa_E1010)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpHps2, trimmed (extracted from Methylovorus gluco…Available sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV INS2x mRFP-3MREs (AAV-beta)
Plasmid#206928PurposeBeta cell-specific gene expression from AAV in human pancreatic isletsDepositorInsertmRFP
UseAAVTagsExpressionMammalianMutationPromoterINSx2Available sinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR myc-MBP-SspB-iRFP-NLS
Plasmid#223286PurposeAn SspB-tagged maltose binding protein (MBP) with an infrared fluorescent protein for visualization. Used to demonstrate condensate dissolution using the OptoMBP system.DepositorInsertMyc-MBP-SspB-irFP-NLS
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-NKX2-1-EF1a-TagRFP-2A-tet3G
Plasmid#167942PurposeInducible NKX2-1 overexpressionDepositorInsertTTF1 (NKX2-1 Human)
UseLentiviralTagsExpressionMutationPromotertetONAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only