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Showing: 1681 - 1700 of 2594 results

  1. JPF0468

    Plasmid
    #124038
    Purpose
    Encodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of tagBFP- and mScarlet-targeting sgRNAs in a 2nd gen. Lenti Virus destination vector
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    pHR_pEF1a-iRFP713_mScarletsgRNA_tagBFP sgRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124038-plasmid-map-sequence-id-252546

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  2. MTK0_016

    Plasmid
    #123931
    Purpose
    Encodes the phiC31 attB GFP dropout destination vector constitutive NLS::tagBFP as a type 0 part to be used in the MTK system
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    Phic31attB tagBFP destination vector
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    123931-plasmid-map-sequence-id-250083

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  3. Tol2-CAG::Cytbow

    Plasmid
    #158991
    Purpose
    Multicolor cytoplasmic cell labeling with a genome-integrating Tol2 transposon vector (randomly expressing 3 tdTomato, mCerulean or mEYFP upon Cre recombination)
    Depositor
    Jean Livet
    Article
    Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10
    Insert
    Cytbow
    Use
    Tags
    The default expressed EBFP2 is fused to H2B
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    158991-plasmid-map-sequence-id-311790

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  4. Tol2-CAG::Palmbow

    Plasmid
    #158993
    Purpose
    Multicolor membrane-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing palmitoylated mCherry, mEYFP or mCerulean upon Cre recombination)
    Depositor
    Jean Livet
    Article
    Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10
    Insert
    Palmbow
    Use
    Tags
    The default expressed EBFP2 is fused to H2B; oth…
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    158993-plasmid-map-sequence-id-311799

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  5. Tol2-CAG::Mitbow

    Plasmid
    #158994
    Purpose
    Multicolor mitochondrial-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing mito-tethered mEYFP, mCherry or mCerulean upon Cre recombination)
    Depositor
    Jean Livet
    Article
    Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10
    Insert
    Mitbow
    Use
    Tags
    The default expressed EBFP2 is fused to H2B; oth…
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    158994-plasmid-map-sequence-id-311800

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  6. pGC(2.1)-U6.3

    Plasmid
    #170516
    Purpose
    PCR template vector for amplifying gRNAcore(2.1)-pU6.3 promoter fragment; used together with pAC-CR7T-gRNA2.1-nlsBFP
    Depositor
    Chun Han
    Article
    Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118
    Insert
    gRNAcore(2.1)-pU6.3
    Use
    Pcr template vector for amplifying grnacore(2.1)-…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    170516-plasmid-map-sequence-id-334635

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  7. pSR04

    Plasmid
    #69151
    Purpose
    read-outloxP mCherry to GFP switch, with eft-1::tagBFP::tbb-2UTR as gene of interest for integration on cxtTi10816, Mos Chr IV
    Depositor
    Sander van den Heuvel
    Article
    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10
    Inserts
    mCherry
    TagBFP
    eGFP
    Use
    Cre/Lox; Mossci
    Tags
    Expression
    Worm
    Mutation
    codon-optimzed index 1.0
    Promoter
    eef-1A.1 (eft-3) and rps-27
    Available since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    69151-plasmid-map-sequence-id-125046

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  8. pTR(EF)-tRNA(Q)

    Plasmid
    #170517
    Purpose
    PCR template vector for amplifying gRNAcore(EF)-tRNA(Q) fragment; used together with pAC-U63-tgRNA-nlsBFP or pAC-U63-tgRNA-Gal80
    Depositor
    Chun Han
    Article
    Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118
    Insert
    gRNAcore(EF)-tRNA(Q)
    Use
    Pcr template vector for amplifying grnacore(ef)-t…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    170517-plasmid-map-sequence-id-334636

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  9. pUASTLOTattB_vhhGFP4::T48-Baz::mCherry

    Plasmid
    #163927
    Purpose
    Expression of GrabFP-A-extracellular under control of UAS or LOP enhancer
    Depositor
    Markus Affolter
    Article
    Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife
    Insert
    Fusion of vhhGPF4 (extracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor
    Use
    Cre/Lox
    Tags
    mCherry
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    163927-plasmid-map-sequence-id-323456

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  10. pUASTLOTattB_mCherry::T48-Baz::vhhGFP4

    Plasmid
    #163928
    Purpose
    Expression of GrabFP-A-intracellular under control of UAS or LOP enhancer
    Depositor
    Markus Affolter
    Article
    Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife
    Insert
    Fusion of vhhGPF4 (intracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor
    Use
    Cre/Lox
    Tags
    mCherry
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    163928-plasmid-map-sequence-id-323457

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  13. attB53_SNCB+_attB53

    Plasmid
    #183614
    Purpose
    RMCE vector to shuttle puromycin resistance, anti-GFP SynNotch receptor, tagBFP and TRE-mCherry cassettes into attP50-flanked landing pad (Addgene 183609). All cassettes on sense (+) strand.
    Depositor
    Sally Lowell
    Article
    Malaguti et al Development. 2022 May 26. pii: 275525. d
    Inserts
    LaG17 GFP nanobody SynNotch tTA
    TRE-mCherry-SV40pA
    tagBFP
    Use
    Rmce
    Tags
    Myc and human CD8a signal sequence
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    183614-plasmid-map-sequence-id-361603

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  14. attB53_SNCB-_attB53

    Plasmid
    #183615
    Purpose
    RMCE vector to shuttle puromycin resistance (+), anti-GFP SynNotch receptor (+), tagBFP (-) and TRE-mCherry (-) cassettes into attP50-flanked landing pad (Addgene 183609). (+) = sense (-) = antisense.
    Depositor
    Sally Lowell
    Article
    Malaguti et al Development. 2022 May 26. pii: 275525. d
    Inserts
    LaG17 GFP nanobody SynNotch tTA
    TRE-mCherry-SV40pA
    tagBFP
    Use
    Rmce
    Tags
    Myc and human CD8a signal sequence
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    183615-plasmid-map-sequence-id-366916

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  17. ACE2 g1 + Cas9 (Lentiviral Prep)

    Viral Prep
    #153011-LV
    Purpose
    Ready-to-use Lentiviral Prep particles produced from ACE2 g1 + Cas9 (#153011). In addition to the viral particles, you will also receive purified ACE2 g1 + Cas9 plasmid DNA. Lentiviral particles carrying a guide RNA targeting ACE2 and co-expressing Cas9 and TagBFP2.
    Depositor
    Jason Sheltzer
    Promoter
    Tags
    None
    Available since
    Sept. 14, 2020
    Availability
    Academic Institutions and Nonprofits only

  18. TMPRSS2 g1 + Cas9 (Lentiviral Prep)

    Viral Prep
    #153015-LV
    Purpose
    Ready-to-use Lentiviral Prep particles produced from TMPRSS2 g1 + Cas9 (#153015). In addition to the viral particles, you will also receive purified TMPRSS2 g1 + Cas9 plasmid DNA. Lentiviral particles carrying a guide RNA targeting TMPRSS2 and co-expressing Cas9 and TagBFP2.
    Depositor
    Jason Sheltzer
    Promoter
    Tags
    None
    Available since
    Sept. 14, 2020
    Availability
    Academic Institutions and Nonprofits only

  19. PB-TO-ASCL1-DLX2

    Plasmid
    #182307
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into GABAergic neuron via ASCL1 and DLX2 expression
    Depositor
    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)
    Inserts
    Ascl1 (Ascl1 Mouse)
    Dlx2 (Dlx2 Mouse)
    puroR-T2A-mycNLS-mTagBFP2
    Use
    Tags
    None and T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and pTRE3G-bidirectional
    Available since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    182307-plasmid-map-sequence-id-359517

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Showing: 1681 - 1700 of 2594 results