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Plasmid#1186DepositorInserthtt 103Q (HTT Human)
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only
These results may also be relevant.
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pRA1SEGFPTcIfm
Plasmid#193777PurposeCassette 1: Expresses EGFP under the control of PR promoter, Cassette 2: Expresses frame-shifted CI under the control of PLTetO-1 promoter, pUC origin of replication, Ampicillin selectionDepositorInsertEGFP and frame-shifted CI in opposite orientation, controlled by separate promoters and terminators
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPR promoter and PLTetO-1Available sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTA1cI-T3
Plasmid#193789PurposeExpresses truncated version of lambda CI named T3 under the control of PLTetO-1 promoter, pUC origin of replication, Ampicillin selectionDepositorInsertTruncated version of Lambda CI, named T3, where the first two codons are deleted
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLTetO-1Available sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
UG76-pLYS2-GFP-PKCbeta
Plasmid#184762PurposeMarker for DAG. Yeast expression of human PKC beta under LSY2 promoter. Uses auxotrophic marker TRP1(Kluyveromyces lactis).DepositorInsertpLYS2-GFP-PKCbeta (PRKCB Budding Yeast, Human)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pShuttle.Cre
Plasmid#192930PurposeGateway compatible vector with BsaI cloning site and Cre expressionDepositorTypeEmpty backboneUseGateway-compatible cloning vectorTagsExpressionMutationPromoterCMVAvailable sinceDec. 9, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKLV2-EF1a-GFP ArgSen delta Degradation Domain
Plasmid#184692PurposeLentiviral transfer plasmid for expression of GFP ArgSen delta Degradation DomainDepositorInsertGFP ArgSen delta Degradation Domain
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-GFP ArgSen delta Disordered Region
Plasmid#184693PurposeLentiviral transfer plasmid for expression of GFP ArgSen delta Disordered RegionDepositorInsertGFP ArgSen delta Disordered Region
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-GFP ArgSen delta Rad23b UbL
Plasmid#184694PurposeLentiviral transfer plasmid for expression of GFP ArgSen delta Rad23b UbLDepositorInsertGFP ArgSen delta Rad23b UbL
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-GFP ArgSen delta Degradation Domain
Plasmid#184701PurposeLentiviral transfer plasmid for expression of GFP ArgSen delta Degradation DomainDepositorInsertGFP ArgSen delta Degradation Domain
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-GFP ArgSen delta Disordered Region
Plasmid#184702PurposeLentiviral transfer plasmid for expression of GFP ArgSen delta Disordered RegionDepositorInsertGFP ArgSen delta Disordered Region
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMM4_14L
Plasmid#183063PurposePlasmid expressing a biosensor for acetic acid and pHluorin: BM3R1-Haa1-mTurquoise2 under RET2p, mCherry under HREsYGP1p and sfpHluorin under TDH3p with flanks for genome integration into HO locusDepositorInsertsmCherry
sfpHluorin
BM3R1-HAA1-mTurquoise2
UseSynthetic BiologyTagsmCherry and mTurquoise2ExpressionYeastMutationPromoterRET2, Synthetic promoter, and TDH3Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pk_MSP10-Cd4-bio-His
Plasmid#126940PurposeExpression of recombinant P. knowlesi protein in mammalian cellsDepositorInsertMSP10 (PKH_112880 Plasmodium knowlesi)
UseTagsratCd4(d3+4)-bio-6HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pk_P41-Cd4-bio-His
Plasmid#126847PurposeExpression of recombinant P. knowlesi protein in mammalian cellsDepositorInsertP41 (PKH_030970 Plasmodium knowlesi)
UseTagsratCd4(d3+4)-bio-6HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pk_P92-Cd4-bio-His
Plasmid#126848PurposeExpression of recombinant P. knowlesi protein in mammalian cellsDepositorInsertP92 (PKH_110660 Plasmodium knowlesi)
UseTagsratCd4(d3+4)-bio-6HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pk_GAMA-Cd4-bio-His
Plasmid#126842PurposeExpression of recombinant P. knowlesi protein in mammalian cellsDepositorInsertGAMA (PKH_050210 Plasmodium knowlesi)
UseTagsratCd4(d3+4)-bio-6HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pk_MSP4-Cd4-bio-His
Plasmid#126843PurposeExpression of recombinant P. knowlesi protein in mammalian cellsDepositorInsertMSP4 (PKH_041300 Plasmodium knowlesi)
UseTagsratCd4(d3+4)-bio-6HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pk_MSP5-Cd4-bio-His
Plasmid#126844PurposeExpression of recombinant P. knowlesi protein in mammalian cellsDepositorInsertMSP5 (PKH_041310 Plasmodium knowlesi)
UseTagsratCd4(d3+4)-bio-6HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pk_P12-Cd4-bio-His
Plasmid#126845PurposeExpression of recombinant P. knowlesi protein in mammalian cellsDepositorInsertP12 (PKH_113620 Plasmodium knowlesi)
UseTagsratCd4(d3+4)-bio-6HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pk_P38-Cd4-bio-His
Plasmid#126846PurposeExpression of recombinant P. knowlesi protein in mammalian cellsDepositorInsertP38 (PKH_102490 Plasmodium knowlesi)
UseTagsratCd4(d3+4)-bio-6HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pm_P12-Cd4-bio-His
Plasmid#126822PurposeExpression of recombinant P. malariae protein in mammalian cellsDepositorInsertP12 (P12p Plasmodium malariae)
UseTagsratCd4(d3+4)-bio-6HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
psiCheck2-SV40p-mtIF3(SR)-3xFLAG-HSVTKp-mCherry
Plasmid#182381PurposeDual expression construct encoding shRNA-resistant mtIF3 and mCherry from separate promotersDepositorInsertsUseTags3xFlagExpressionMammalianMutationChanged CDS sequence from 'ccacgttcaagtcacga…PromoterHSV TK promoter and SV40 promoterAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only