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Plasmid#172991Purposeconstitutive expression of FLAG-tagged TAOK1DepositorInsert3xFLAG-TAOK1 (TAOK1 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER21-puro_TAOK2-D151A
Plasmid#172980Purposedoxycycline-inducible expression of kinase-dead TAOK2DepositorInsertkinase-dead TAOK2 (TAOK2-D151A) (TAOK2 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationD151APromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#1
Plasmid#172983PurposeCRISPRi for TAOK2DepositorInsertsgTAOK2i (TAOK2 Human)
UseCRISPR and LentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-A11(2-196, G38R)-Strep
Plasmid#196209PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G38R)-Strep.DepositorInsertAnnexin A11 (ANXA11 Human)
UseTagsGB1-6xHis-TEV and Strep-II tagExpressionBacterialMutationG38RPromoterAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-A11(2-196, D40G)-Strep
Plasmid#196210PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, D40G)-Strep.DepositorInsertAnnexin A11 (ANXA11 Human)
UseTagsGB1-6xHis-TEV and Strep-II tagExpressionBacterialMutationD40GPromoterAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-A11(2-196, G175R)-Strep
Plasmid#196211PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G175R)-Strep.DepositorInsertAnnexin A11 (ANXA11 Human)
UseTagsGB1-6xHis-TEV and Strep-II tagExpressionBacterialMutationG175RPromoterAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-A11(2-196, G189E)-Strep
Plasmid#196212PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G189E)-Strep.DepositorInsertAnnexin A11 (ANXA11 Human)
UseTagsGB1-6xHis-TEV and Strep-II tagExpressionBacterialMutationG189EPromoterAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM30-C9ORF78
Plasmid#193367PurposeExpression of C9ORF78 in E.coli with N-terminal His6-GST-tag which is cleavable with TEV proteaseDepositorInsertC9ORF78 (C9orf78 Human)
UseTagsHis6-GST-TEVExpressionBacterialMutationPromoterT7Available sinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
ATX3-D1
Plasmid#184246PurposeRecombinant protein expression and purification. Encodes ataxin-3 Josephin domain and UIM1 fused with His-Tag and TEV cleavage sequence (N-terminal)DepositorInsertAtaxin-3 Josephin domain and UIM1 (ATXN3 Human)
UseTags6x His-Tag and TEV cleavage siteExpressionBacterialMutationPremature stop codon N263XPromoterT7 promoterAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
KS6A1_HUMAN_D1
Plasmid#79720PurposeThis plasmid encodes the kinase domain of KS6A1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertKS6A1 (RPS6KA1 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
KC1G3_HUMAN_D0
Plasmid#79685PurposeThis plasmid encodes the kinase domain of KC1G3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertKC1G3 (CSNK1G3 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET mCerulean LIC cloning vector (H6-mCerulean)
Plasmid#29726DepositorTypeEmpty backboneUseTagsBiotin - His6 - TEV and mCeruleanExpressionBacterialMutationPromoterAvailable sinceNov. 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pKG333
Plasmid#69751PurposeRetroviral expression of Histone H3DepositorInsertHistone H3
UseRetroviralTagseGFP-TEV-SExpressionMammalianMutatione.coli optimized sequencePromoterAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
RP1B-PP1gamma
Plasmid#51770PurposeExpresses PP1 gamma residues 7-323DepositorInsertpp1 gamma
UseTagsHis, TEV protease site, and Thio-6ExpressionBacterialMutationContains PP1 gamma residues 7-323PromoterT7-lacAvailable sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
DPYSL3A-c010
Plasmid#53641PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertDPYSL3A
UseBaculovirus transfer vectorTagsHis6-ZBasic-TEVExpressionMutationPromoterpolyhedrin promoterAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL
Plasmid#62304PurposeIt is a donor vector for generation of recombinant baculovirus by site-specific transposition in an E. coli host.DepositorTypeEmpty backboneUseBaculovirus expressionTags6xHis and TEV cleavage siteExpressionMutationPromoterpolyhedrinAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
RP1B-PP1beta
Plasmid#51769PurposeExpresses PP1 beta residues 6-327DepositorInsertpp1 beta
UseTagsHis, TEV protease site, and Thio-6ExpressionBacterialMutationContains PP1 beta residues 6-327PromoterT7-lacAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
ACVR1A-c076
Plasmid#53691PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertACVR1A
UseBaculovirus expressionTagsHis6-TEVExpressionMutationPromoterpolyhedrin promoterAvailable sinceJune 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
SNX27A-c006
Plasmid#53662Purposebacterial expression plasmidDepositorInsertSNX27A
UseTagsHis6-TEVExpressionBacterialMutationBacterial expression for structure determination;…PromoterAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
SNX3A-c018
Plasmid#53665Purposebacterial expression plasmidDepositorInsertSNX3A
UseTagsHis6-TEVExpressionBacterialMutationBacterial expression for structure determination;…PromoterAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only